FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692514

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692514
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1379115
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC39430.28590799171932724No Hit
CCTATGTACTGACTCAGCCACCCTCAGTGTCAGTGGCCCCAGGAGAGACG37910.27488643079076075No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT29980.21738578726212102No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG28700.20810447279595973No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC28360.20563912364088563No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCACAAGATCACTATAATCATCCCACAG27900.20230365125460895No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27440.19896817886833223No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG24510.17772266997313496No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCACAAGATCACTA22230.1611903285802852No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT21840.15836242807887665No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG21500.15589707892380258No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20330.14741337741957705No Hit
GGATTACCTGTGGGGATGACAATTTTGGAACTAAAAGTGTGCACTGGTACCAGCAGAAGACAGGCCAGGCCC19690.14277272018649642No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT18990.13769700133781446No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG18600.13486910083640596No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC17830.1292858101028558No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC17790.12899576902578827No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG17700.1283431766023863No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC16780.1216722318298329No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC16460.11935190321329257No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT16360.11862680052062374No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG15990.11594392055774899No Hit
CTATTACTGTCAGCTGTGGGATGATTATAGTGATCTTGTGGTCTTCGGCG15840.11485626651874571No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA15540.11268095844073917No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC15170.10999807847786444No Hit
GGCCAGGCCCCTGTGTTGGTCATCTATTATGATAGAGACCGGCCCTCAGG14520.10528491097551691No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAACG601.2187229E-1050.6131252
TACGTAT901.6698505E-646.72671570
CTAACGC652.6739144E-1046.7079473
GGTATCA19450.046.683361
GTATCAA53100.038.0769161
TATCAAC61450.032.6164322
GTGGTAT5200.032.4560851
ATCAACG61700.032.366683
TCAACGC62150.031.9683364
CAACGCA63250.031.4135065
AACGCAG63750.031.2752236
TGGTATC5600.030.7293972
CGCAGAG67800.029.4567728
ACGCAGA67700.029.450457
AGAGTAC69550.028.90961311
ATGGCAA8550.028.69184370
CAGAGTA70000.028.5791810
GCAGAGT71100.028.2319249
GTACGTA1304.866615E-727.69619669
ACGCCAT1104.5542038E-827.6021546
Produced by FastQC (version 0.11.5)