FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692515

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692515
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1093828
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGCAGCATGGGATGACAGCCTGTATGTCTTCGGAACTGGGACCAAAGTCACCGTCCTAGGTCAG81500.7450897216015681No Hit
GTAATAATCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGG77280.706509615771401No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT53420.48837660034301555No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA50860.4649725551000706No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG41110.3758360546630732No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC40170.36724238180042934No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC38840.35508324892030557No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC38790.35462613866165427No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC34400.3144918579520729No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC32200.2943790065714171No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC31890.29154492296777923No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT29720.2717063377423141No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC29620.2707921172250116No Hit
GACCTAGGACGGTGACTTTGGTCCCAGTTCCGAAGACATACAGGCTGTCATCCCATGCTGCACAGTAATAATCAG29460.2693293643973276No Hit
GCCTTGGGCTGACCTAGGACGGTGACTTTGGTCCCAGTTCCGAAGACATA28680.2621984443623678No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT25660.2345889847398311No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT25560.23367476422252856No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACTTTGGTCCCAGTTCCGAAGACATACAGG25530.2334004980673378No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT24480.22380118263566118No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG24170.22096709903202333No Hit
ACCTAGGACGGTGACTTTGGTCCCAGTTCCGAAGACATACAGGCTGTCATCCCATGCTGCACAGTAATAATCAGC23180.21191631591072818No Hit
CCCCTGAGGGCCGCTGATTATTCCTATAGATGAGGACTTTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAGTATA22440.20515108408268942No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC22220.20313979894462383No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG22170.20268268868597256No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG22010.20121993585828848No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC21510.1966488332717758No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG21270.19445470403024973No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG21010.19207773068526313No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC20960.19162062042661188No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG20670.1889693809264345No Hit
GTAATACACAGCCGTGTCCTCGTCTGTCAGGCTGTTCATTTGCAGATACA20280.1854039209089546No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA18930.17306194392537036No Hit
GATTATTCCTATAGATGAGGACTTTGGGGGCCGTTCCTGGGAGCTGCTGG17730.16209129771773992No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA17680.16163418745908864No Hit
GGATGACAGCCTGTATGTCTTCGGAACTGGGACCAAAGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCCA17640.16126849925216763No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA17170.15697166282084568No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC15590.1425269786474656No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC15480.1415213360784328No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG15380.14060711556113029No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGT15320.14005858325074874No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC15160.13859583042306467No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC15060.13768160990576217No Hit
GCCTGTATGTCTTCGGAACTGGGACCAAAGTCACCGTCCTAGGTCAGCCC14840.13567032476769655No Hit
GATGAGGACTTTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAGTATACAT14350.13119064423291413No Hit
GGCCATCAGTGGGCTCCGGTCCGAGGATGAGGCTGATTATTACTGTGCAGCATGGGATGACAGCCTGTATGTCTT14340.1310992221811839No Hit
CTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGTAATTATGTATACTGGTACCAGCAGCTCCCAGGAACGGCCCC14220.13000215756042083No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG14130.12917935909484854No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT14120.1290879370431183No Hit
ATCCCATGCTGCACAGTAATAATCAGCCTCATCCTCGGACCGGAGCCCAC14100.1289050929396578No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT13910.12716807395678295No Hit
GCATGGGATGACAGCCTGTATGTCTTCGGAACTGGGACCAAAGTCACCGT13590.12424256830141484No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGTCCGAGGATG13420.12268839342200054No Hit
GAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCT13400.12250554931854003No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC13390.12241412726680977No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC13380.12232270521507951No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA13360.12213986111161901No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT13330.12186559495642826No Hit
GTGTATTACTGTGCGAGAGTGGCCACGGCAGTAACTCCTGATTACTGGGGCCAGGGAACCCTGGTCACCGTCTC13270.12131706264604672No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT13170.1204028421287442No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG12850.11747733647337606No Hit
ATGCTGCACAGTAATAATCAGCCTCATCCTCGGACCGGAGCCCACTGATG12700.11610600569742227No Hit
GTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTC12660.11574031749050126No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC12640.11555747338704075No Hit
GACATACAGGCTGTCATCCCATGCTGCACAGTAATAATCAGCCTCATCCT12530.11455183081800795No Hit
GTATACATAATTACTTCCGATGTTGGAGCTGCTTCCAGAACAAGAGATGGTGACCCTCTGCCCGGGGGTCCCAGA12400.11336334414551465No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG12400.11336334414551465No Hit
GTCCGAGGATGAGGCTGATTATTACTGTGCAGCATGGGATGACAGCCTGTATGTCTTCGGAACTGGGACCAAAGT12350.11290623388686338No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG12170.11126063695571882No Hit
CTGTCATCCCATGCTGCACAGTAATAATCAGCCTCATCCTCGGACCGGAG12010.10979788412803476No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG11970.10943219592111375No Hit
TTACTGTGCAGCATGGGATGACAGCCTGTATGTCTTCGGAACTGGGACCA11910.1088836636107322No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG11850.1083351313003507No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC11530.10540962564498257No Hit
TTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCA11380.10403829486902877No Hit
GTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGCTG11250.10284980819653547No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA11230.10266696409307496No Hit
GTGCAGCATGGGATGACAGCCTGTATGTCTTCGGAACTGGGACCAAAGTC11180.1022098538344237No Hit
CCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAG11020.10074710100673964No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG10970.10028999074808836No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGAC1650.051.57953622
ATGCCGT1350.051.31067344
TCGTATG1500.050.50626440
TATCTCG1450.049.6570836
CGTATGC1550.048.947241
CACGAGA1750.048.6299121
GACGGAC1700.048.09517326
CGAGACG1800.047.2877323
TCTCCGA1800.047.27475711
TTACGCC306.7404157E-445.3899963
ATCTCGT1600.045.04321337
CCCACGA1950.043.6422319
GGTATCA11250.043.576391
GCCAGAG27100.043.31752870
GTGGTAT3150.043.230541
ATCTCCG2000.042.54728310
TCTGGAG7800.041.95775670
GCCGTCT1700.040.84359746
CGGACTC2150.039.6441128
CCTATCT1900.039.6296134
Produced by FastQC (version 0.11.5)