FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692519

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692519
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1237814
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGCTGTACACTGGTACCAGCAGCTTCCAGGAACAGC61880.4999135572872823No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCTACCACTCAGGCTGCTGTCATAGGA49400.3990906549772421No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC42920.3467403018547213No Hit
CCCTACCACTCAGGCTGCTGTCATAGGACTGGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGTGATGG40730.32904782140127675No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG36670.296248063117722No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC36270.2930165598385541No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT35050.28316047483709184No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCTACCACTCAGG32900.2657911447115641No Hit
GGTTATGCTGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTCCTCATCTATGGTGACAACAATCGG31720.25625821003801863No Hit
GTCATAGGACTGGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAG31550.2548848211443723No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA29960.24203959560967964No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG25740.20794723601445775No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG23970.19364783400413957No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG23810.19235523269247237No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC23780.1921128699465348No Hit
GTGGTAGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC23390.18896215424934604No Hit
GTAGTATAGGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA22860.1846804124044485No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT22720.1835493862567397No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG22470.1815296967072597No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC22360.18064103330548853No Hit
GTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC22320.18031788297757173No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC22190.17926764441184218No Hit
GGCCATCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGT21630.17474353982100702No Hit
GTCCTATGACAGCAGCCTGAGTGGTAGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG21530.17393566400121505No Hit
GGGTCACCCTCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGCTGTACACTGGTACCAGCAGCTTC21360.17256227510756866No Hit
GGGCAGAGGGTCACCCTCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGCTGTACACTGGTACCAG20980.1694923469923591No Hit
GATTATTACTGCCAGTCCTATGACAGCAGCCTGAGTGGTAGGGTGTTCGG20950.16924998424642151No Hit
GCTGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTCCTCAT20860.16852289600860873No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20760.16771502018881673No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA20190.16311012801600241No Hit
GTGCAGGAGAGGGTGACCCTCTGCCCTGGGGCCCCAGACACTGAGGGCGG20050.16197910186829362No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT19850.16036335022870965No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA19790.15987862473683445No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG19680.15898996133506327No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGT19590.15826287309725048No Hit
GTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTCCTCATCTA19060.15398113125235294No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT18960.15317325543256097No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCTACCACTCAGGC18630.1505072652272474No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18410.14872993842370502No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA18290.14776048743995462No Hit
CTATACTACTGTGTGAAATTGGGGGGATTACGATATTTTGACTGGAGAAT18190.14695261162016263No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGTGATGGCCAGGGAGG17870.1443674089968283No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC17820.14396347108693228No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC17470.14113590571766033No Hit
AGGTTATGCTGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAAC16740.1352384122331788No Hit
TCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGTCCTAT16440.13281478477380285No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA16430.13273399719182366No Hit
GGATGAGGCTGATTATTACTGCCAGTCCTATGACAGCAGCCTGAGTGGTAGGGTGTTCGGCGGAGGGACCAAG16410.13257242202786523No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT16340.13200690895401085No Hit
ATGCTGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTCCTCATCTATGGTGACAACAATCGGCCCT16240.13119903313421888No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA15950.12885619325682213No Hit
GTGTCTGGGGCCCCAGGGCAGAGGGTCACCCTCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATG15780.12748280436317572No Hit
GCCTCATCCTCAGCCTGGAGCCCAGTGATGGCCAGGGAGGCTGAGGTGCC15770.12740201678119653No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC15760.12732122919921735No Hit
ATACTACTGTGTGAAATTGGGGGGATTACGATATTTTGACTGGAGAATGGACGTCTGGGGCCAAGGGACCACGGT15740.12715965403525895No Hit
CAGTAGTATAGGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATA15680.12667492854338375No Hit
GCCTGGAGCCCAGTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCC15380.12425130108400778No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC15240.12312027493629898No Hit
GCATAACCTGCCCCGATGTTGGAGCTGCTCCCAGTGCAGGAGAGGGTGACCCTCTGCCCTGGGGCCCCAGACA15020.12134294813275662No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCTAC14860.12005034682108945No Hit
CATCTATGGTGACAACAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTC14720.11891932067338065No Hit
AGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGT14240.11504151673837912No Hit
CTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTC14150.11431442850056632No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT14120.11407206575462872No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCACTGGGCTCCAGGCTGAGGATG13880.11213316378712795No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC13660.11035583698358559No Hit
GTATAGGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACACCG13480.10890166050796No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG13330.10768984677827204No Hit
GCCCTATACTACTGTGTGAAATTGGGGGGATTACGATATTTTGACTGGAG13290.10736669645035521No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA13190.10655882063056324No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC13060.10550858206483366No Hit
ACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGC12850.10381204284327047No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC12830.10365046767931209No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC12820.10356968009733288No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGCTGCTGTGCCATGAGCTGGGTCCGCCAGGCTCCAGGAAAGGGG12710.10268101669556168No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC12650.1021962912036865No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC12630.1020347160397281No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT12530.10122684021993611No Hit
TCCAGGAACAGCCCCCAAACTCCTCATCTATGGTGACAACAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGG12390.10009581407222734No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTT150.00238939468.7524638
ATTCCGT252.5106053E-455.44985244
TATTCCG252.5233836E-455.39290643
TTCCGTC200.007190178652.0548945
CTCCGTA200.00776132251.058216
CGTGAAA505.6765202E-849.55392557
ACTAGCG4850.049.2036927
CTAGCGC5000.049.1011628
TCTCGTA1850.048.3125438
GGTATCA10650.048.271331
TAGCGCT4950.048.25066829
TACGTAT605.292675E-447.6687670
CTTATAC5700.047.186111
TATACAC5900.046.7406733
CGCTCAT4350.046.38114532
GACTAGC5350.045.8610926
CACGAGA5500.045.8087621
ACGAGAC5550.045.4015722
TCGTATG2400.044.51886440
CCCACGA5850.044.22670419
Produced by FastQC (version 0.11.5)