FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692520

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692520
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1237814
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGCTGTACACTGGTACCAGCAGCTTCCAGGAACAGC62280.5031450605664501No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG49010.3959399392800534No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC41610.3361571286154463No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCTACCACTCAGGCTGCTGTCATAGGA40570.32775522008960956No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG37510.30303422000397473No Hit
GTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCCTCTCCTG34770.28089842254167424No Hit
CCCTACCACTCAGGCTGCTGTCATAGGACTGGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGTGATGG32590.26328672967020894No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT31810.2569852982758314No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC30190.24389770999520122No Hit
GGTTATGCTGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTCCTCATCTATGGTGACAACAATCGG29330.23694997794499012No Hit
GTGGTAGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC28260.22830570667321587No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA25790.20835117392435376No Hit
GTCATAGGACTGGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAG25220.20374628175153942No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG25090.20269604318580983No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG24770.20011084056247544No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC24450.1975256379391411No Hit
GGGTCACCCTCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGCTGTACACTGGTACCAGCAGCTTC23720.1916281444546596No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCTACCACTCAGG23590.19057790588893No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG22780.1840341117486149No Hit
GTAGTATAGGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA22610.1826607228549685No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGT22570.1823375725270517No Hit
GTCCTATGACAGCAGCCTGAGTGGTAGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG22030.17797504310017498No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC21790.1760361411326742No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT21730.175551415640799No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG21260.17175439928777667No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC20900.1688460463365255No Hit
GGCCATCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGT20790.16795738293475432No Hit
GGGCAGAGGGTCACCCTCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGCTGTACACTGGTACCAG20700.16723029469694156No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA19600.15834366067922967No Hit
GCTGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTCCTCAT19540.1578589351873545No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19450.1571318469495417No Hit
GATTATTACTGCCAGTCCTATGACAGCAGCCTGAGTGGTAGGGTGTTCGG19270.15567767047391612No Hit
CTGCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCAC19180.15495058223610333No Hit
CTATACTACTGTGTGAAATTGGGGGGATTACGATATTTTGACTGGAGAAT18790.15179986653891456No Hit
GTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCAC18740.15139592862901857No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG18640.1505880528092266No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT18600.1502649024813098No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG18530.1496993894074554No Hit
GTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTCCTCATCTA18370.14840678809578822No Hit
GCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACC18180.14687182403818344No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC17760.1434787455950571No Hit
GTGCAGGAGAGGGTGACCCTCTGCCCTGGGGCCCCAGACACTGAGGGCGG17740.1433171704310987No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT17510.14145905604557713No Hit
GTGTCTGGGGCCCCAGGGCAGAGGGTCACCCTCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATG17340.14008566715193074No Hit
GTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC17330.14000487956995156No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA16950.136934951454742No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA16860.1362078632169292No Hit
ATACTACTGTGTGAAATTGGGGGGATTACGATATTTTGACTGGAGAATGGACGTCTGGGGCCAAGGGACCACGGT16150.13047194489640607No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT15880.12829068018296771No Hit
GGATGAGGCTGATTATTACTGCCAGTCCTATGACAGCAGCCTGAGTGGTAGGGTGTTCGGCGGAGGGACCAAG15840.12796752985505092No Hit
AGGTTATGCTGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAAC15810.12772516710911333No Hit
AGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGT15700.12683650370734215No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC15480.12505917690379978No Hit
ATGCTGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTCCTCATCTATGGTGACAACAATCGGCCCT15070.1217468860426526No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA15060.1216660984606734No Hit
TCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGTCCTAT14960.12085822264088142No Hit
GTATAGGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACACCG14770.11932325858327664No Hit
CTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTC14630.11819223243556785No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC14580.11778829452567187No Hit
GGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGC14540.11746514419775508No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC14410.11641490563202549No Hit
CAGTAGTATAGGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATA14390.1162533304680671No Hit
CATCTATGGTGACAACAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTC14390.1162533304680671No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCACTGGGCTCCAGGCTGAGGATG14340.11584939255817109No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC13720.11084056247546077No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCTACCACTCAGGC13690.11059819972952317No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGTGATGGCCAGGGAGG13600.10987111149171039No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCTAC13380.10809378468816802No Hit
ACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGC13340.10777063436025122No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG13270.10720512128639682No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC12990.10494306899097926No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC12880.10405440558920807No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT12810.10348889251535368No Hit
TCCAGGAACAGCCCCCAAACTCCTCATCTATGGTGACAACAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGG12800.10340810493337449No Hit
GCCCTATACTACTGTGTGAAATTGGGGGGATTACGATATTTTGACTGGAG12800.10340810493337449No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT12730.10284259185952009No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGCTGCTGTGCCATGAGCTGGGTCCGCCAGGCTCCAGGAAAGGGG12550.10138841538389451No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA12500.10098447747399852No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)