FastQCFastQC Report
Wed 8 May 2024
EGAF00004692521

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692521
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1169762
Total Bases86.6 Mbp
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAGTTACCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCA105810.9045429754086729No Hit
CTATTATACTGTTGGCAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCT100310.8575248640321707No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA53880.4606065165392618No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT50690.4333360119408905No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC47900.40948500635171936No Hit
GATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAAGTTCAGCG36190.30937917285738464No Hit
GTGTATTACTGTGCGACTAAGTCCAAATACTGGGGCCAGGGAACCCTGGT36150.30903722295646463No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC34240.2927091151875339No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC31890.27261955850848296No Hit
AGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTC31450.26885810959836276No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29570.252786464255122No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG29300.25047830242391184No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG28810.2462894161376417No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGCATTAGCAA27710.23688579386234124No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT27130.231927520299001No Hit
GCATAGATCAGGGACTTAGGGGCTTTCCCTGGTTTCTGCTGAAACCAGGC26700.22825155886411083No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA25760.22021573619249044No Hit
CAGTATAATAGTTACCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGAT23430.2002971544638995No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22900.1957663182767093No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22790.19482595604917924No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA22730.19431303119779922No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22520.1925177942179691No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTC22040.18841439540692892No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG21860.18687562085278886No Hit
GTCTCCATCCTCACTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT21730.1857642836747988No Hit
GTCCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAAGT21460.1834561218435887No Hit
GATCAGGGACTTAGGGGCTTTCCCTGGTTTCTGCTGAAACCAGGCTAAAT20590.17601871149857834No Hit
GCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGT20290.1734540872416782No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA19980.1708039755095481No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG19850.16969263833155807No Hit
ATTATACTGTTGGCAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGC19220.1643069273920678No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGG19000.16242620293700769No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC18850.16114389080855762No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18620.15917767887826756No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG18490.1580663417002775No Hit
CAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCT18230.1558436673442974No Hit
GGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTTCC18160.15524525501768735No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA18060.15439038026538732No Hit
CAGTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATA17560.1501160065038871No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGA17540.14994503155342712No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGTAGCTATTGGATGATCTGGG17210.14712394487083696No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG17180.14686748244514697No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT17010.1454141953662369No Hit
AGTCAGGGCATTAGCAATTATTTAGCCTGGTTTCAGCAGAAACCAGGGAA16960.14498675799008687No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC16960.14498675799008687No Hit
GTATTTGGACTTAGTCGCACAGTAATACACAGCCGTGTCCTCGGCTCTCA16820.14378993333686682No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC16580.1417382339313467No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC16490.14096884665427667No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG16070.1373783726946165No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTA16070.1373783726946165No Hit
GGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCT16060.1372928852193865No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15670.13395887368541634No Hit
GACCCAGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGG15540.13284753650742628No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCT15410.13173619932943625No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGC15180.12976998739914614No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAATTGGGGCTGAGCTGGG15160.12959901244868616No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15140.12942803749822612No Hit
GCTCCTGGGGCTCCTGCTGCTCTGTTTCCCAGGTGCCAGATGTGACATCC15030.1284876752706961No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG14890.12729085061747605No Hit
GTCCAAATACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCC14740.126008538489026No Hit
GTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCC14730.12592305101379597No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14460.12361488918258587No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT14420.12327293928166584No Hit
ATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAAGTTCAGCGGCAGT14250.12181965220275578No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGATCATCCAATAGCTAC14050.12010990269815569No Hit
TCCTCACTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGTCGGGC14000.11968246532200567No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13980.11951149037154565No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA13780.11780174086694559No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT13740.11745979096602556No Hit
GGGCTGGAGTGGGTGGCCATCATAAACCAAGATGGAAGTGAGAAATACTA13730.11737430349079556No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG13640.11660491621372551No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG13500.11540809156050547No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13480.11523711661004547No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC13410.11463870428343544No Hit
GTATTACTGTGCGACTAAGTCCAAATACTGGGGCCAGGGAACCCTGGTCA13250.11327090467975537No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCA13130.11224505497699531No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC12870.11002238062101523No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12840.10976591819532522No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA12810.1095094557696352No Hit
GTTTCCCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCA12790.10933848081917517No Hit
GCCTTAGCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGC12550.10728678141365508No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12450.10643190666135503No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT12370.10574800685951502No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT12050.10301240765215487No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC12030.10284143270169488No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC11930.10198655794939483No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA11870.1014736330980148No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG11780.10070424582094477No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)