FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692523

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692523
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1108228
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT37710.3402729402252966No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30180.2723266331476916No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATTCCA28230.2547309759363596No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC26730.24119585500456583No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26170.23614274319002948No Hit
CTCCTGTAGGGCCAGTCAGAGTGTAAGTTACTACTTAGCCTGGTACCAACAGACACCTGGCCAGGCTCCCAGGCT24600.2219759832814186No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAGGATT24060.21710333974597285No Hit
GCCTAGAGCCTGAGGATTTTGCAGTTTATTACTGTCAGCAGCGTAGCGAC23840.21511818867597643No Hit
GTCTCCAGACACACTGTCTTTGTCTCCGGGGGACACAGCCACCCTCTCCTGTAGGGCCAGTCAGAGTGTAAGTTA22550.20347798467463377No Hit
GTCTTTGTCTCCGGGGGACACAGCCACCCTCTCCTGTAGGGCCAGTCAGAGTGTAAGTTACTACTTAGCCTGGTA21920.19779323388328032No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21880.19743229732509918No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20980.18931122476602286No Hit
GTCCCAGACCCACTGCCACGGAACCTGTCTGGGGTGCCAATGGCCCTGTT19960.18010734253240307No Hit
GAGTGTAAGTTACTACTTAGCCTGGTACCAACAGACACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATC19300.1741518893224138No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18320.16530894364697518No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18180.1640456656933411No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18140.1636847291351599No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17880.16133864150698232No Hit
GTAATATGTAGCCGTGTCCTGAGTTGTCAGGTTGTTCATTTGGAGAAACA17710.15980466113471234No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17370.15673670039017243No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17010.15348827136654192No Hit
GTTTATTACTGTCAGCAGCGTAGCGACTGGCCTCGGATCACCTTCGGCCA16700.15069101304063784No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16140.14563790122610148No Hit
GTAATAAACTGCAAAATCCTCAGGCTCTAGGCTGCTGATGGTGAGAGTGA15940.14383321843519564No Hit
GTCGTGTCCCTTGGCCGAAGGTGATCCGAGGCCAGTCGCTACGCTGCTGA15630.1410359601092916No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAGGATTTTGCAGTTTATTACTGTCAGCAGCGTAGCGACTGG15620.1409457259697463No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15160.13679495555066284No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14670.13237348271294355No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACGGAACCTGTCTGGGGTGCCA14350.1294859902474942No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT14240.128493414712496No Hit
GGACACGACTGGACATTAGACGAACTGTGGCTGCACCATCTGTCTTCATC13970.1260570929447731No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC13960.12596685880522782No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13170.11883836178114973No Hit
ACATATTACTGTGTGAAAGATCCGGGCCAGCGCCTATCCGGCCTATACAG12940.116762976571608No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12890.11631180587388155No Hit
TCTATGATGCATCCAACAGGGCCATTGGCACCCCAGACAGGTTCCGTGGCAGTGGGTCTGGGACAGACTTCACT12820.1156801668970645No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC12720.11477782550161157No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12710.1146875913620663No Hit
CCCCAGACAGGTTCCGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACC12700.114597357222521No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT12500.11279267443161514No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12210.11017588438480168No Hit
CGACTGGACATTAGACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT12180.10990518196616579No Hit
CTGCTACTCTGGCTCCCAGATTCCACCGGAGAAATTGTATTGACACAGTC12150.10963447954752993No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12120.10936377712889404No Hit
CTCCAGACACACTGTCTTTGTCTCCGGGGGACACAGCCACCCTCTCCTGT12090.10909307471025818No Hit
GTATTGACACAGTCTCCAGACACACTGTCTTTGTCTCCGGGGGACACAGC11980.10810049917525998No Hit
CTACAGGAGAGGGTGGCTGTGTCCCCCGGAGACAAAGACAGTGTGTCTGG11900.10737862605889764No Hit
CAGCCACCCTCTCCTGTAGGGCCAGTCAGAGTGTAAGTTACTACTTAGCCTGGTACCAACAGACACCTGGCCAGG11820.1066567529425353No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11700.1055739432679918No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11600.10467160187253886No Hit
CCTTCGGCCAAGGGACACGACTGGACATTAGACGAACTGTGGCTGCACCA11480.10358879219799537No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11360.10250598252345186No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11210.10115247043027246No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)