FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692524

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692524
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1108228
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT39710.358319768134355No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATTCCA27360.24688060579591925No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27180.24525639128410398No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26470.23884976737638827No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25100.22648769025868323No Hit
CTCCTGTAGGGCCAGTCAGAGTGTAAGTTACTACTTAGCCTGGTACCAACAGACACCTGGCCAGGCTCCCAGGCT24970.22531464644459445No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAGGATT23510.21214046207098178No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23010.20762875509371717No Hit
GTCTTTGTCTCCGGGGGACACAGCCACCCTCTCCTGTAGGGCCAGTCAGAGTGTAAGTTACTACTTAGCCTGGTA22900.20663617955871896No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22430.20239517500009024No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22380.20194400430236376No Hit
GCCTAGAGCCTGAGGATTTTGCAGTTTATTACTGTCAGCAGCGTAGCGAC21750.19625925351101037No Hit
GTCTCCAGACACACTGTCTTTGTCTCCGGGGGACACAGCCACCCTCTCCTGTAGGGCCAGTCAGAGTGTAAGTTA20200.1822729618814901No Hit
GAGTGTAAGTTACTACTTAGCCTGGTACCAACAGACACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATC18410.1661210509028828No Hit
GTCCCAGACCCACTGCCACGGAACCTGTCTGGGGTGCCAATGGCCCTGTT18160.1638651974142505No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17580.15863161732062356No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17070.15402967620381366No Hit
GTAATATGTAGCCGTGTCCTGAGTTGTCAGGTTGTTCATTTGGAGAAACA17020.15357850550608718No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16500.14888633024973202No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAGGATTTTGCAGTTTATTACTGTCAGCAGCGTAGCGACTGG16410.14807422299382436No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16320.14726211573791675No Hit
GTCGTGTCCCTTGGCCGAAGGTGATCCGAGGCCAGTCGCTACGCTGCTGA15950.14392345257474093No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15950.14392345257474093No Hit
GTTTATTACTGTCAGCAGCGTAGCGACTGGCCTCGGATCACCTTCGGCCA15940.14383321843519564No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15820.14275040876065215No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15210.13724612624838933No Hit
GTAATAAACTGCAAAATCCTCAGGCTCTAGGCTGCTGATGGTGAGAGTGA14600.1317418437361265No Hit
GGACACGACTGGACATTAGACGAACTGTGGCTGCACCATCTGTCTTCATC14210.12822271229386012No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACGGAACCTGTCTGGGGTGCCA14070.12695943434022602No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14040.12668873192159014No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14010.12641802950295428No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13980.1261473270843184No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13760.12416217601432197No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC13250.11956023489751205No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT13070.11793602038569681No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT12710.1146875913620663No Hit
CTGCTACTCTGGCTCCCAGATTCCACCGGAGAAATTGTATTGACACAGTC12550.11324384512934162No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12310.11107822578025461No Hit
GTATTGACACAGTCTCCAGACACACTGTCTTTGTCTCCGGGGGACACAGC11720.10575441154708237No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11590.10458136773299356No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11270.10169387526754421No Hit
CCCCAGACAGGTTCCGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACC11230.10133293870936305No Hit
TCTATGATGCATCCAACAGGGCCATTGGCACCCCAGACAGGTTCCGTGGCAGTGGGTCTGGGACAGACTTCACT11170.10079153387209129No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11130.10043059731391013No Hit
ACATATTACTGTGTGAAAGATCCGGGCCAGCGCCTATCCGGCCTATACAG11110.10025012903481954No Hit
GGCCGAAGGTGATCCGAGGCCAGTCGCTACGCTGCTGACAGTAATAAACTGCAAAATCCTCAGGCTCTAGGCTG11110.10025012903481954No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)