FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692526

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692526
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1472298
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC47970.32581719190000935No Hit
GCCTTGGGCTGACCCAGGACGGTCAGCGTGGTCCCGCCGCCGAACACAGTGCTGCTGCTTGCAGATGAGCCGCCA32960.2238677224311926No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT31270.2123890679740107No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG30980.21041935803757122No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30170.20491775442199883No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG29600.20104625558141084No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC29030.19717475674082283No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG25310.17190813272856448No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG24400.1657273187900819No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT24360.16545563466091784No Hit
CTCCTGGACAGTCGGTCACCATCTCCTGCACTGGAACCAGCGGTGACATT23980.1628746354338592No Hit
GTGCTGCTGCTTGCAGATGAGCCGCCACCCGCATATGAGCTGCAATAATAATCAGCCTCGTCCTCAGCCTGGAGG22330.15166766510584134No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGGTCACCATCTCCTG22140.150377165492312No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT21510.14609814045797792No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC19900.1351628542591242No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT18690.1269444093519111No Hit
CTCCTGCACTGGAACCAGCGGTGACATTGGTGGTTATAACTTTGTCTCGTGGTTTCAACAACATCCGGGCAGAGC18640.126604804190456No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT18250.12395588393110633No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG18170.1234125156727782No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC18070.12273330534986802No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCGTGGTCCCGC17820.12103527954259259No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA17500.11886180650928005No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT17050.11580536005618428No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC16980.11532991283014717No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA16920.11492238663640104No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG16870.11458278147494597No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC16720.11356396599058072No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC16620.11288475566767055No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACAAT400.006702214552.9859570
GGTATCA21550.041.386551
GTATCAA54450.036.8545571
CGCGGTT4800.036.795870
TATCAAC63050.031.5907232
ATCAACG63300.031.5171493
TCAACGC63700.031.2153744
CAACGCA65050.030.6734315
AACGCAG65500.030.5657846
TCGTTGT500.00576106129.3168564
CGCAGAG69300.028.7923038
ACGCAGA69900.028.593467
CAGAGTA70950.027.93237510
AGAGTAC71400.027.89818611
GTGGTAT6800.027.8215731
GCAGAGT76650.026.0313999
GATATAC1601.0186341E-1025.3375051
TACGTAT1400.001161959125.23140370
CGTCATA4000.024.48376718
GAGTACG52850.023.5053112
Produced by FastQC (version 0.11.5)