FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692527

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692527
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1181341
Sequences flagged as poor quality0
Sequence length20-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC31520.2668154241662653No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT24870.21052346443575565No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT20110.17023027220760137No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG19540.1654052470878434No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT18580.1572788889914089No Hit
CTGTTATACTTTTGACAGTAATAAGTTGCAACATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC18420.1559244959753365No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18360.15541659859430937No Hit
GTATAACAGTGGCCTCGGGGTTTTTTTCGGCCCTGGGACCAAAGTGGACATCAAACGAACTGTGGCTGCACCAT17600.14898323176796538No Hit
CCATATACGAGTGGGACCACAGCTGTGTAGCACTTGTTTCTGTCATAAGT16750.1417880188700807No Hit
ATGTAAGTGTGCCCGGATTACTTCCAGGATCATCCGTTCTTCCGAAGATCCTAATGAGGACATTGTGGAGAGAAA15130.12807478958234753No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14830.12553530267721175No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC13370.11317646640555097No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT13270.11232997077050572No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13110.1109755777544333No Hit
GTGTAGCACTTGTTTCTGTCATAAGTGTAGCAGGTCTCTGTAGCACTGTC13100.11089092819092877No Hit
GTTCATGTGAAAGCCCAAGAAGATGAAAGGATTGTTCTTGTTGACAACAA12540.10615055263467535No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12480.10564265525364819No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12480.10564265525364819No Hit
GATCTATGCTGCATCCACTTTGCAATCAGGGGTCCCATCTCGGTTCAGTGGCAGGGGATCTGGGACAGATTTCA12380.10479615961860292No Hit
ATATACGAGTGGGACCACAGCTGTGTAGCACTTGTTTCTGTCATAAGTGT12240.10361106572953957No Hit
CTGCTACCCAGAGCAATATCTGTGATGAAGACAGTGCTACAGAGACCTGC11850.10030973275286306No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG3550.044.6732740
GACGCGT4150.043.87884526
GTCGTAT950.043.43765639
ACGCGTA4250.042.8864127
CGCGTAG4300.042.42374428
CGTATGC3600.042.24920341
AGACGCG4550.041.48604625
ATGCCGT3650.040.0461744
CGAGACG4800.040.0038123
GAGACGC4850.039.60146324
ATCTCGT3100.037.560137
CCCACGA5300.037.48203319
ACGAGAC5200.036.92033822
CCGTCTT3800.036.895347
CGTAGTA4900.036.6423230
CACGAGA5550.035.19443521
TAGTAAT5050.034.3131432
AATCTCG3300.034.18120236
GCGTAGT5450.034.14401629
CTAACGC808.810093E-833.6603243
Produced by FastQC (version 0.11.5)