FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692531

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692531
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1355715
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC41010.30249720627122956No Hit
GTAATAATCGGCCTCGTCCCCAGTCTGGAGTCCGGTGATGCCCAGGGTGG32430.23920956838273533No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT32240.23780809388403906No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAGCAC30640.2260062033687021No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG29500.21759735637652458No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG27390.202033613259424No Hit
GATTATTACTGCGCGGCATGGGATAGCAGCCTGAGTGCCGTGCTATTCGG27110.19996828241924No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAGCACGGCACTCAGG25430.18757629737813628No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC24990.1843307774864186No Hit
GCATGGGATAGCAGCCTGAGTGCCGTGCTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG24630.1816753521204678No Hit
GGATAGCAGCCTGAGTGCCGTGCTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC24060.17747092862437902No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT22960.16935712889508486No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC22100.16301361274309129No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC22090.16293985092737043No Hit
ATCCCATGCCGCGCAGTAATAATCGGCCTCGTCCCCAGTCTGGAGTCCGG21470.15836661835267737No Hit
GTAATACACGGCCGTGTCCTCGACTCCCAGGCTGTTCATTTGCAGATATA21010.15497357482951798No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20870.153940909409426No Hit
CCCAGGAACAGCCCCCAAACTCCTCATTTATGACGATAATAAGCGACCCTCAGGGGTTCCTGACCGATTCTCCGG19890.14671225146878217No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG19600.14457315881287733No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT18880.13926230808097573No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG18820.13881973718665058No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC18740.13822964266088372No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18610.1372707390565126No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG17670.13033712837875217No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC17630.13004208111586876No Hit
GTATGGACGTCTGGGGCCACGGGACCACGGTCACCGTCTTCTCAGCATCCCCGACCAGCCCCAAGGTCTTCCCG17280.1274604175656388No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT17200.12687032303987195No Hit
GTGTATTACTGTGCGAAAGATCTTCACTGGCACGGTATGGACGTCTGGGGCCACGGGACCACGGTCACCGTCTT17000.12539508672545482No Hit
CTGCTATCCCATGCCGCGCAGTAATAATCGGCCTCGTCCCCAGTCTGGAG16580.12229709046517889No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC16570.12222332864945804No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT16080.1186089996791361No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA16060.11846147604769439No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC15850.11691247791755642No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC15710.11587981249746443No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG15620.11521595615597673No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC14610.10776601276817029No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA14430.1064383000851949No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA14390.10614325282231148No Hit
GTCCTGGGCCCAGTCTGTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCATTATCTCCTG14310.10555315829654463No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14210.10481554013933607No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAGCACGGCACTCAGGC14040.10356158927208152No Hit
GTTCCTGGGAGCTGCTGATACCAGGATACATAATAATTTCCAATGTTGGA13680.1009061639061307No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)