FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692532

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692532
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1355715
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC40800.30094820814109163No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG31440.23190714862637057No Hit
GTCCTGGGCCCAGTCTGTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCATTATCTCCTG30910.22799777239316524No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT30430.22445720523856416No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27560.20328756412667853No Hit
GATTATTACTGCGCGGCATGGGATAGCAGCCTGAGTGCCGTGCTATTCGG25360.18705996466809027No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG25260.18632234651088173No Hit
GTAATAATCGGCCTCGTCCCCAGTCTGGAGTCCGGTGATGCCCAGGGTGG25180.18573225198511487No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG25160.18558472835367315No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC24680.18204416119907207No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAGCAC24530.18093773396325924No Hit
GCATGGGATAGCAGCCTGAGTGCCGTGCTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG23980.17688083409861216No Hit
GGATAGCAGCCTGAGTGCCGTGCTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC23460.17304521968112765No Hit
GTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCAT23100.17038979431517687No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT21400.15785028564263137No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20800.15342457669938003No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG20780.15327705306793832No Hit
GTAATACACGGCCGTGTCCTCGACTCCCAGGCTGTTCATTTGCAGATATA20670.15246567309500889No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAGCACGGCACTCAGG20500.15121172222775434No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC20140.14855629686180355No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT20050.14789244052031586No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC19750.14567958604869016No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG18210.1343202664276784No Hit
CCCAGGAACAGCCCCCAAACTCCTCATTTATGACGATAATAAGCGACCCTCAGGGGTTCCTGACCGATTCTCCGG18010.13284503011326126No Hit
GTATGGACGTCTGGGGCCACGGGACCACGGTCACCGTCTTCTCAGCATCCCCGACCAGCCCCAAGGTCTTCCCG17740.13085346108879817No Hit
GTGTATTACTGTGCGAAAGATCTTCACTGGCACGGTATGGACGTCTGGGGCCACGGGACCACGGTCACCGTCTT17450.12871436843289336No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC16810.12399361222675857No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT16320.12037928325643664No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG16060.11846147604769439No Hit
ATCCCATGCCGCGCAGTAATAATCGGCCTCGTCCCCAGTCTGGAGTCCGG16030.11824019060053183No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT15960.11772385789048583No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC15830.1167649542861147No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC15780.11639614520751043No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA15520.11447833799876819No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC15060.1110852944756088No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG14810.10924124908258742No Hit
CTGCTATCCCATGCCGCGCAGTAATAATCGGCCTCGTCCCCAGTCTGGAG14090.10393039835068581No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC13790.10171754387906012No Hit
GCACAGGGTCCTGGGCCCAGTCTGTGTTGACGCAGCCGCCCTCAGTGTCT13730.10127497298473499No Hit
CCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCATTATCTCCTGCTCTGG13560.10002102211748044No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)