FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692533

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692533
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences837825
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGGTAGTGCACCTTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT26960.32178557574672517No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT23630.2820398054486319No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC21840.2606749619550622No Hit
CTACCATACTGGTGACAGTAATAGACTGCAAAATCTTCCGGCTCCAGTCGGTTGATGGTGAGAGTGAAGTCTGTC19760.23584877510219915No Hit
CTATTACTGTCACCAGTATGGTAGTGCACCTTACACTTTTGGCCAGGGGA18020.21508071494643868No Hit
GTATATCACTGTGTGAGAGAGACATTCAGCAGGGGGAGGAATTACGATTT16840.20099662817414138No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16690.19920627816071376No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC15160.18094470802375198No Hit
GTGATATACAGCCGTGTCCTCGACCCTGAGACTATTCAGCTGTAGATACA14560.17378330797004146No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14050.16769611792438754No Hit
GTCTATTACTGTCACCAGTATGGTAGTGCACCTTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA13840.1651896279055889No Hit
CACCTGGCCAGGCGCCCAGGCTCGTCATCTATGGTGCATCCAGCAGGGCC13700.1635186345597231No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC13130.1567153045086981No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12950.15456688449258496No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12470.14883776444961655No Hit
GTTAGGACCCAGAGGGAACCATGGAAAACCCAGCGCAGCTTCTCCTCCTCCTGCTACTCTGGCTCCCAGATACCA12450.1485990511144929No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12390.14788291110912183No Hit
CAGTAATAGACTGCAAAATCTTCCGGCTCCAGTCGGTTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA12120.1446602810849521No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11860.14155700772834423No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11840.14131829439322055No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11740.14012472771760212No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT11510.13737952436367978No Hit
GTAGTGCACCTTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA11230.1340375376719482No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10590.12639871094799032No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT10530.12568257094261928No Hit
GTGCACTACCATACTGGTGACAGTAATAGACTGCAAAATCTTCCGGCTCCAGTCGGTTGATGGTGAGAGTGAAGT10230.12210187091576404No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT9870.11780503088353773No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9780.11673082087548116No Hit
GTAATAGACTGCAAAATCTTCCGGCTCCAGTCGGTTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA9760.11649210754035746No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTACCGGCAAAAGCAACTACTTAGCCT9740.1162533942052338No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG9610.11470175752692985No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG9570.11422433085668249No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC9550.1139856175215588No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG9380.1119565541730075No Hit
GTCTGGGACAGACTTCACTCTCACCATCAACCGACTGGAGCCGGAAGATT9270.11064363082982723No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT9150.10921135081908515No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT9070.1082564974785904No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA8920.10646614746516277No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT8750.10443708411661146No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTACCGG8740.10431772744904963No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACGA150.002474655968.145279
CGAATAG200.006899118452.60015552
CGTCGAC200.007463570751.5644337
CGCTGAA200.00773185551.10590414
GGTATCA8400.043.815521
ACGAGAC2100.042.2002822
CGAGACG2000.040.90181423
GGGGGAT7650.040.07921270
CGTATGC1350.038.40400341
TATCTCG1350.038.16636
CACGAGA2300.037.0465321
TCGTATG1500.036.80767440
CGGACTC2250.036.40280528
GTATCAA22800.034.8260771
AATGGTA5750.034.77572670
GAGTTTA3500.034.0787351
ATCTCGT1550.033.26737637
ACTCCTA2100.032.56870331
CTTATAC2750.032.21991
CCTATCT1950.031.64620434
Produced by FastQC (version 0.11.5)