FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692534

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692534
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences837825
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGGTAGTGCACCTTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT26270.3135499656849581No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT25910.3092531256527318No Hit
CTACCATACTGGTGACAGTAATAGACTGCAAAATCTTCCGGCTCCAGTCGGTTGATGGTGAGAGTGAAGTCTGTC18830.2247486050189479No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18020.21508071494643868No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16940.20219019484975978No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16880.20147405484438877No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16110.19228359144212692No Hit
CTATTACTGTCACCAGTATGGTAGTGCACCTTACACTTTTGGCCAGGGGA15370.18345119804255064No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC15220.18166084802912302No Hit
GTATATCACTGTGTGAGAGAGACATTCAGCAGGGGGAGGAATTACGATTT15210.18154149136156117No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14160.1690090412675678No Hit
GTGATATACAGCCGTGTCCTCGACCCTGAGACTATTCAGCTGTAGATACA13510.16125085787604812No Hit
GTCTATTACTGTCACCAGTATGGTAGTGCACCTTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA12490.14907647778474026No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11680.13940858771223108No Hit
GTTAGGACCCAGAGGGAACCATGGAAAACCCAGCGCAGCTTCTCCTCCTCCTGCTACTCTGGCTCCCAGATACCA11520.1374988810312416No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT11520.1374988810312416No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11480.13702145436099425No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11430.13642467102318503No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11180.133440754334139No Hit
GTGCACTACCATACTGGTGACAGTAATAGACTGCAAAATCTTCCGGCTCCAGTCGGTTGATGGTGAGAGTGAAGT10560.1260406409453048No Hit
GTAGTGCACCTTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA10520.12556321427505743No Hit
CACCTGGCCAGGCGCCCAGGCTCGTCATCTATGGTGCATCCAGCAGGGCC10490.12520514427237192No Hit
CAGTAATAGACTGCAAAATCTTCCGGCTCCAGTCGGTTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA10390.12401157759675349No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10340.12341479425894428No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10280.12269865425357325No Hit
GTAATAGACTGCAAAATCTTCCGGCTCCAGTCGGTTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA10140.12102766090770745No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10100.12055023423746009No Hit
GTCTGGGACAGACTTCACTCTCACCATCAACCGACTGGAGCCGGAAGATT10080.1203115209023364No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9810.11708889087816668No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG9610.11470175752692985No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG9500.11338883418374958No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT9360.1117178408378838No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTACCGGCAAAAGCAACTACTTAGCCT9200.10980813415689433No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9190.1096887774893325No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT9110.10873392414883776No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG8870.10586936412735357No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8850.10563065079222987No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTACCGG8590.10252737743562199No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8510.10157252409512726No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT8450.10085638408975622No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAA305.468881E-447.35523259
GGTATCA9900.042.2867661
GTATCAA26400.038.0323071
GGGGGAT8050.035.92752570
CTAATGG1001.8815142E-434.43054670
TATCAAC29200.034.1379742
ATCAACG30300.032.780493
TCAACGC30850.032.30444
CAACGCA31250.032.003575
AACGCAG31800.031.6639966
GTGTATA652.5459565E-531.4175591
CTCGTGA450.004540809430.7778842
AGAGTAC32950.030.55888211
GGCGAAA450.004951657730.2376910
GTGGTGA6100.029.35062870
GTCGTAA854.047475E-628.74008248
CAGAGTA35750.028.16545710
TTTCACG6200.027.60826149
CGCAGAG36850.027.2323848
ACGCAGA37000.027.213927
Produced by FastQC (version 0.11.5)