FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692535

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692535
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1175078
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTGTACTGGTCATCTATCAAGATACCAAGCGGCCCTCAGGGATCCCT48350.4114620476257746No Hit
GTACAGGGGACTGGCCTGGCTTCTGCTGATACCAGCAAGCATATTTATCC36830.3134260023589923No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC35220.2997247842270896No Hit
GCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCAC28140.239473464740213No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT25920.22058110185026017No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTCCAGATAAA24550.20892230132808204No Hit
GTGTATTACTGTGCGAGAGATCGGTGTAGTGGTGGTAGTTGCTACTCTTTGGACTGGTACTTCGATTTCTGGGGC23020.19590188906608752No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG22310.18985973697065217No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT17240.14671366496521934No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC17150.1459477583615726No Hit
CCTATGAGTTGACTCAGCCACCCTCAGTGTCCGTGTCCCCAGGACAGACA16370.13930990112996755No Hit
GTGTAGTGGTGGTAGTTGCTACTCTTTGGACTGGTACTTCGATTTCTGGG15230.12960841748377555No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTC15170.1290978130813444No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC14970.12739579840657386No Hit
CCCTCAGTGTCCGTGTCCCCAGGACAGACAGCCAGCATCACCTGCTCTGG14690.1250129778618951No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14560.12390666832329429No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG14380.1223748551160008No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13970.11888572503272123No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG13350.11360947954093262No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT13240.1126733714698088No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC12740.1084183347828825No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT12710.10816303258166692No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC12580.10705672304306608No Hit
CCCCAGGACAGACAGCCAGCATCACCTGCTCTGGAGATAAATTGGGGGAT12540.10671632010811197No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT12160.10348249222604797No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG12150.10339739149230945No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12120.10314208929109386No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA11920.10144007461632333No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG11910.10135497388258483No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT11770.10016356361024545No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT11770.10016356361024545No Hit
GTACTGGTCATCTATCAAGATACCAAGCGGCCCTCAGGGATCCCTGAGCG11760.10007846287650693No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTATCG250.00122918381.2852270
ATCGCGG451.6702017E-460.21127370
AGGTGAT13800.040.24992870
GACGGAC2200.038.793826
GTATCAA34400.037.2935831
TCGTATG1950.037.1362240
CGAGACG2400.036.96453523
TCTCCGA2550.034.78127311
TATGGCG400.00255065734.62512243
CGTATGC1800.034.526741
CCCACGA2800.032.898319
TATCAAC39200.032.3761022
ACTCTGG23250.032.33928370
ATCAACG39150.032.330313
TCAACGC39500.031.9574664
CAACGCA39850.031.8480115
AACGCAG40150.031.6950176
TCCGAGC3050.031.31630113
ATCTCCG3100.030.81120110
GTAGTTT1552.4872515E-630.59121370
Produced by FastQC (version 0.11.5)