FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692536

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692536
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1175078
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTGTACTGGTCATCTATCAAGATACCAAGCGGCCCTCAGGGATCCCT45650.3884848495163725No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC34560.2941081358003469No Hit
GTACAGGGGACTGGCCTGGCTTCTGCTGATACCAGCAAGCATATTTATCC34190.2909594086520214No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG25480.216836669565765No Hit
GCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCAC25380.21598566222837973No Hit
GTGTATTACTGTGCGAGAGATCGGTGTAGTGGTGGTAGTTGCTACTCTTTGGACTGGTACTTCGATTTCTGGGGC24100.20509276830984838No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT23780.20236954483021552No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTCCAGATAAA23750.20211424262899993No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18290.1556492420077646No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC17430.14833057890625134No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16100.13701218131902734No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16080.13684197985155028No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG16050.1365866776503347No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT15570.13250184243088545No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC14640.1245874741932025No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT14290.12160894851235408No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTC14220.1210132433761844No Hit
GTGTAGTGGTGGTAGTTGCTACTCTTTGGACTGGTACTTCGATTTCTGGG14010.11922612796767533No Hit
CCTATGAGTTGACTCAGCCACCCTCAGTGTCCGTGTCCCCAGGACAGACA13960.1188006242989827No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC13650.1161625015530884No Hit
CCCTCAGTGTCCGTGTCCCCAGGACAGACAGCCAGCATCACCTGCTCTGG12990.11054585312634566No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG12510.1064610179068964No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA12330.10492920469960292No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT12250.10424839882969472No Hit
GACTATTACTGTCAGACGTGGGACAGCAGCACTGTGGTCTTCGGCGGAGGGACCAAGCTGACCGTCCTGGGTCAG11880.10109967168136923No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGC507.635026E-847.722663
AACCGTA306.692568E-445.4559567
GTGGTAT4300.037.299831
GTATCAA32250.036.7178461
GCTAGTT450.00257112934.56836768
CCTAACG601.4556776E-534.094872
ATCAACG36600.031.5729543
ACTCTGG23000.031.50969170
TCAACGC36850.031.3600924
TATCAAC37200.031.3452842
CAACGCA37600.030.7371735
AACGCAG38300.030.3534226
CGACAAT450.004895635430.30913216
AGGTGAT11900.029.86495670
GCGTGTT7850.029.80816751
CGCAGAG40050.029.1973678
ACGCAGA40400.028.691267
AGAGTAC41350.028.2794311
CAGAGTA41950.027.95622410
AAGCGTG8600.027.9428349
Produced by FastQC (version 0.11.5)