FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692537

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692537
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences599531
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAAAGACAGTGAGAGGCCCTCAGGGGTCCCTGAGCGATTCTCTGGCT36130.6026377284911039No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAC28490.47520478507366587No Hit
GTCTTTATACATCACCACCACAGGGGCCTGGCCTGGCTTCTGCTGGTACC24620.41065432813315744No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC24050.40114689649075697No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACATAAGTACGA22740.37929648341787164No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG18290.3050717977886047No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC18150.30273663913959414No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT17900.2985667129806466No Hit
GGAATATACTTATTGGTACCAGCAGAAGCCAGGCCAGGCCCCTGTGGTGGTGATGTATAAAGACAGTGAGAGGCC17490.2917280340799725No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG16350.2727131707951716No Hit
CCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATGTATTGTCAAAG15680.26153776868919204No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC14210.2370186028745803No Hit
CCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACG13470.22467562144409545No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC13460.22450882439773756No Hit
CTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGG12770.21299982819904226No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACATAAGTACGAC12490.20832951090102095No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT12470.20799591680830515No Hit
GGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCAC12130.20232481723213647No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG11750.19598652947053616No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG11270.18798027124535677No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT11230.18731308305992517No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG11100.18514472145727243No Hit
CTATTATTGTCAATCAGCAGACAGCAGTCGTACTTATGTGGTATTCGGCG11080.18481112736455663No Hit
GTATATTCCTTTGACAATACATCTCCAGAGCAGGTGATCCTGGCCGTCTG11050.18431073622548294No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC10580.17647127504666146No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA10040.1674642345433347No Hit
GTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATGT9860.16446188770889245No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA9480.15812359994729214No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG9470.15795680290093425No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG9410.15695602062278682No Hit
GTCCAGGAGGCAGAACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTAC9380.1564556294837131No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC9310.15528805015920777No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC9280.15478765902013408No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT8890.14828257421217586No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG8800.14678140079495472No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC8670.14461303919230198No Hit
GGCCAGGCCCCTGTGGTGGTGATGTATAAAGACAGTGAGAGGCCCTCAGG8280.13810795438434376No Hit
TCCTCACTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCA8250.13760756324527004No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG8090.13493881050354362No Hit
CCCCTGTGGTGGTGATGTATAAAGACAGTGAGAGGCCCTCAGGGGTCCCT7890.13160286957638556No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT7790.1299348991128065No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA7690.12826692864922748No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC7690.12826692864922748No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA7510.12526458181478523No Hit
CCTCAGGGGTCCCTGAGCGATTCTCTGGCTCCAGCTCAGGGACAACAGTCACGTTGACCATCAGTGGAGTCCAGG7450.12426379953663781No Hit
CAATAATAGTCAGCCTCGTCTTCTGCCTGGACTCCACTGATGGTCAACGT7310.12192864088762716No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG7260.12109465565583764No Hit
CTCTACTTCTCCCCCTCCTCACTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGT7210.12026067042404814No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC7060.11775871472867959No Hit
CCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAATAAGTATATTCCTTT7020.11709152654324796No Hit
TCAGTGGAGTCCAGGCAGAAGACGAGGCTGACTATTATTGTCAATCAGCA6950.11592394721874266No Hit
GTCCAGGCAGAAGACGAGGCTGACTATTATTGTCAATCAGCAGACAGCAG6840.11408917970880572No Hit
CTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGT6810.11358878856973201No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA6640.11075323878164764No Hit
GCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCT6630.11058644173528975No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG6560.10941886241078443No Hit
GCTCTGGAGATGTATTGTCAAAGGAATATACTTATTGGTACCAGCAGAAG6460.10775089194720541No Hit
GGCCTGGCCTGGCTTCTGCTGGTACCAATAAGTATATTCCTTTGACAATACATCTCCAGAGCAGGTGATCCTGGC6340.10574932739091056No Hit
GCTTGGTCCCTCCGCCGAATACCACATAAGTACGACTGCTGTCTGCTGATTGACAATAATAGTCAGCCTCGTCTT6210.10358096578825782No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT6190.103247371695542No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT6100.10174619827832089No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG6060.10107901009288928No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT6040.10074541600017346No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT6040.10074541600017346No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC6030.10057861895381556No Hit
CCACATAAGTACGACTGCTGTCTGCTGATTGACAATAATAGTCAGCCTCG6020.10041182190745766No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGT554.3655746E-1156.1534937
CGCTAAT554.5474735E-1155.87038832
GTCTTTA4250.054.2549931
TCGCTAA653.6379788E-1252.45718831
TTTATAC4650.048.1254124
TATACAT4800.048.0342676
ATGGCAA1850.047.26591570
CTTTATA4900.046.3620073
ACTCGCT752.0008883E-1145.38292329
TCTCGTA704.6020432E-1044.1877738
ACGTACC1200.042.3619713
TTATACA5400.042.069235
TGCCGTC758.058123E-1041.72541445
GTATGCC856.548362E-1140.67753242
CGTACCA1250.040.6674914
ATACGTA1250.040.6674911
CGTATGC856.548362E-1140.61532241
TCGTATG856.730261E-1140.53267340
CACTCGC857.6397555E-1140.0269928
TACGTAC1300.039.1033612
Produced by FastQC (version 0.11.5)