FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692538

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692538
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences599531
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAAAGACAGTGAGAGGCCCTCAGGGGTCCCTGAGCGATTCTCTGGCT33340.5561013525572489No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAC27110.4521867926762753No Hit
GTCTTTATACATCACCACCACAGGGGCCTGGCCTGGCTTCTGCTGGTACC26730.445848504914675No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC22160.36962225472911325No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG20470.3414335538946276No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACATAAGTACGA19460.3245870522124794No Hit
GGAATATACTTATTGGTACCAGCAGAAGCCAGGCCAGGCCCCTGTGGTGGTGATGTATAAAGACAGTGAGAGGCC17410.2903936577091093No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC16710.27871786446405605No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT16710.27871786446405605No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG15580.259869798225613No Hit
CCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATGTATTGTCAAAG14150.23601782059643286No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC14050.23434985013285384No Hit
CTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGG13510.22534280962952707No Hit
GGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCAC12960.2161689720798424No Hit
CCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACG12540.2091634961328105No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT12460.20782911976194723No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC12270.20465997588114707No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12050.20099044086127324No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG11590.19331777672880968No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG11530.19231699445066225No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG11180.18647909782813565No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT11090.18497792441091454No Hit
GTATATTCCTTTGACAATACATCTCCAGAGCAGGTGATCCTGGCCGTCTG10910.18197557757647229No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC10850.18097479529832486No Hit
CTATTATTGTCAATCAGCAGACAGCAGTCGTACTTATGTGGTATTCGGCG10570.17630447800030358No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG10270.1713005666095665No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG9660.1611259467817344No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACATAAGTACGAC9480.15812359994729214No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA9380.1564556294837131No Hit
GTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATGT9310.15528805015920777No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC9210.15362007969562877No Hit
GTCCAGGAGGCAGAACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTAC9100.15178531218569183No Hit
GGCCAGGCCCCTGTGGTGGTGATGTATAAAGACAGTGAGAGGCCCTCAGG8830.14728179193402843No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC8420.1404431130333544No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8360.13944233075520698No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA8220.13710717210619633No Hit
CTCTACTTCTCCCCCTCCTCACTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGT7950.13260365185453296No Hit
CCCCTGTGGTGGTGATGTATAAAGACAGTGAGAGGCCCTCAGGGGTCCCT7850.13093568139095393No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT7640.12743294341743797No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG7490.12493098772206943No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA7300.12176184384126926No Hit
GCTCTGGAGATGTATTGTCAAAGGAATATACTTATTGGTACCAGCAGAAG7300.12176184384126926No Hit
GTCCAGGCAGAAGACGAGGCTGACTATTATTGTCAATCAGCAGACAGCAG7280.12142824974855344No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT7250.12092785860947976No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC7160.11942668519225862No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC7110.1185926999604691No Hit
TCCTCACTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCA7090.11825910586775329No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA7020.11709152654324796No Hit
CCTCAGGGGTCCCTGAGCGATTCTCTGGCTCCAGCTCAGGGACAACAGTCACGTTGACCATCAGTGGAGTCCAGG6920.11542355607966895No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT6900.11508996198695313No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA6900.11508996198695313No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC6610.11025284764257395No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC6600.11008605059621604No Hit
CTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGT6560.10941886241078443No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT6560.10941886241078443No Hit
GGCCTGGCCTGGCTTCTGCTGGTACCAATAAGTATATTCCTTTGACAATACATCTCCAGAGCAGGTGATCCTGGC6440.10741729785448959No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT6400.10675010966905799No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG6380.10641651557634216No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC6320.10541573329819476No Hit
TCAGTGGAGTCCAGGCAGAAGACGAGGCTGACTATTATTGTCAATCAGCA6300.10508213920547894No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG6240.10408135692733153No Hit
CCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAATAAGTATATTCCTTT6240.10408135692733153No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG6150.10258018351011039No Hit
GTGGAGTCCAGGCAGAAGACGAGGCTGACTATTATTGTCAATCAGCAGAC6120.1020797923710367No Hit
GACTATTATTGTCAATCAGCAGACAGCAGTCGTACTTATGTGGTATTCGG6090.10157940123196299No Hit
GCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCT6090.10157940123196299No Hit
GCTTGGTCCCTCCGCCGAATACCACATAAGTACGACTGCTGTCTGCTGATTGACAATAATAGTCAGCCTCGTCTT6080.10141260418560509No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC6050.10091221304653138No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT6030.10057861895381556No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC6030.10057861895381556No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA6020.10041182190745766No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)