FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692539

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692539
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1382247
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAAGAACTGGCCCATGTGCACTTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT106910.7734507653118436No Hit
GTTCTTATACTGCTGACAGTAATAGACTCCAAAATCTCCAGACTGCAGGCTGCTGATGGTGAGAGTGAACT81710.5911389209019806No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT78190.5656731394606029No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC60030.43429285793349526No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACTT54310.39291096309125645No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC51190.3703390204500353No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG44830.3243269835275461No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC44660.32309710203747954No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGGAGATT43690.31607954294709995No Hit
CTATTACTGTCAGCAGTATAAGAACTGGCCCATGTGCACTTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAA42290.305951107146552No Hit
CTCCTGCAGGGCCAGTGAAAGAATTATCAGCCACGTGGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT41430.29972935372621534No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGACACCAGTGGCCCTGGT40580.2935799462758827No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT40450.2926394486658318No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGGAGATTTTGGAGTCTATTACTGTCAGCAGTATAAGAACTGG39180.28345151047533473No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGTCACCCTCTCCTGCAGGGCCAGTGAAAGAATTATCAG38950.2817875531652447No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG38330.277302103025002No Hit
GTCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACTCTCAC35080.25378966277373No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC35010.25328324098370264No Hit
CAGTAATAGACTCCAAAATCTCCAGACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTCCCAGACCCACTGCCA34980.2530662030736909No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT34480.24944890457349517No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG33840.2448187624932447No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT33380.24149084787306466No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA32510.23519674848272412No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC32300.23367748311264197No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG31890.23071129834248147No Hit
GTCTGTGTCTCCAGGGGAAAGAGTCACCCTCTCCTGCAGGGCCAGTGAAAGAATTATCAGCCACGTGGCCTGGTA31370.22694930790227796No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG29740.21515691479163998No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT28140.20358155959101376No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCCTGGCCAAAAGTGCACATGGGCCAGTTCTTATACTG27560.19938549333078673No Hit
GCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCATCATCTATCGTGTATCCACCAGGGCCACTGGTGTC27530.19916845542077502No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC26950.194972389160548No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGACACCA26220.1896911333502623No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT25810.18672494858010183No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC25780.18650791067009007No Hit
GTCTATTACTGTCAGCAGTATAAGAACTGGCCCATGTGCACTTTTGGCCA25560.18491629933000397No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC25490.1844098775399766No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG24850.1797797354597261No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT24550.17760935635960867No Hit
GTCTGGGACACCAGTGGCCCTGGTGGATACACGATAGATGATGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTA24510.17731997247959302No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA24080.17420909576942473No Hit
GGGGAAAGAGTCACCCTCTCCTGCAGGGCCAGTGAAAGAATTATCAGCCACGTGGCCTGGTACCAGCAGAAAC23600.17073648920923684No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG23130.16733622861905287No Hit
GAGTCACCCTCTCCTGCAGGGCCAGTGAAAGAATTATCAGCCACGTGGCCTGGTACCAGCAGAAACCTGGCCAGG22790.16487646563891983No Hit
GTAATAGACTCCAAAATCTCCAGACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTCCCAGACCCACTGCCA22670.16400831399887286No Hit
CTTCTGGAGAAATGGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGTCACCCTCTC22580.16335720026883763No Hit
ATCGTGTATCCACCAGGGCCACTGGTGTCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACTCTCA22400.16205497280876718No Hit
GAACTGGCCCATGTGCACTTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT22250.16096978325870845No Hit
GGCCAAAAGTGCACATGGGCCAGTTCTTATACTGCTGACAGTAATAGACTCCAAAATCTCCAGACTGCAGGCTG22190.16053570743868498No Hit
CTTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCA22080.15973990176864195No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21550.1559055653584345No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT21170.15315641849828576No Hit
GGCCAGTTCTTATACTGCTGACAGTAATAGACTCCAAAATCTCCAGACTGCAGGCTGCTGATGGTGAGAGTGAA21150.15301172655827794No Hit
CCAGTGGCCCTGGTGGATACACGATAGATGATGAGCCTGGGAGCCTGGCC20880.15105838536817226No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG20860.15091369342816444No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA20190.1460665134379022No Hit
CTGCTACTCTGGCTCCCAGATACTTCTGGAGAAATGGTGATGACGCAGTC20050.1450536698578474No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA19810.14331736657775346No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA19740.14281094478772607No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT19460.14078525762761648No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT19180.1387595704675069No Hit
GGCCACTGGTGTCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGAGT19130.1383978406174873No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC18610.1346358501772838No Hit
CTCCCAGGCTCATCATCTATCGTGTATCCACCAGGGCCACTGGTGTCCCA18520.13398473644724856No Hit
GCAGAAACCTGGCCAGGCTCCCAGGCTCATCATCTATCGTGTATCCACCAGGGCCACTGGTGTCCCAGACAGGTT17610.1274012531768924No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA17410.12595433377681411No Hit
CCCTGTCTGTGTCTCCAGGGGAAAGAGTCACCCTCTCCTGCAGGGCCAGTGAAAGAATTATCAGCCACGTGGC17320.1253032200467789No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT17290.12508618213676717No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT17290.12508618213676717No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC17220.12457976034673977No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT17140.12400099258670846No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA17080.12356691676668498No Hit
AGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCT16740.1211071537865519No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC16680.12067307796652842No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG16560.11980492632648146No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC16500.11937085050645797No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA16490.11929850453645403No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT15820.11445132454619182No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACTTCTGGAGAAATGGTGATGACGCAGTCTC15650.11322144305612528No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA15420.11155748574603526No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG15410.11148513977603135No Hit
GCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCCACGTGGCTGATA15390.11134044783602352No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA15260.11039995022597265No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG15010.10859130097587479No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC14970.10830191709585914No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT14970.10830191709585914No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT14880.1076508033658239No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC14770.10685499769578086No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC14760.10678265172577694No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC14700.10634857590575346No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC14640.10591450008572997No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC14380.10403350486562822No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC14140.10229720158553429No Hit
GTACCAGGCCACGTGGCTGATAATTCTTTCACTGGCCCTGCAGGAGAGGGTGACTCTTTCCCCTGGAGACACAGA14110.10208016367552254No Hit
GGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC14100.10200781770551863No Hit
CAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATA14090.10193547173551472No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAGAT200.006792844752.80890754
CGTCTAG200.007787202551.01553325
GTACGAA306.227798E-446.12532843
ACGCGTA2300.045.8666127
CGCGTAG2400.045.39644228
AGACGCG2450.044.42168825
GACGCGT2400.043.94117726
GCGTAGT2550.042.7493129
CATACGC405.463718E-542.485263
ATCTCGT1950.040.4678737
CGTATGC2000.039.65022341
TAACCGG350.001447821338.85912722
GGTATCA14550.038.5489041
CGAGACG2750.038.3306223
GTATCAA35250.037.995151
CCCACGA2800.037.6311619
ACGAGAC2900.037.51915722
CGTAGTA2750.037.18297630
GAGACGC3000.036.2711524
TCGTATG2200.035.99149740
Produced by FastQC (version 0.11.5)