FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692540

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692540
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1382247
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAAGAACTGGCCCATGTGCACTTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT102980.7450187991003056No Hit
GTTCTTATACTGCTGACAGTAATAGACTCCAAAATCTCCAGACTGCAGGCTGCTGATGGTGAGAGTGAACT88550.6406235643846577No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT80610.58318086420155No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACTT52360.37880349894049326No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC51600.37330520522019583No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC50460.3650577646397496No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC44210.3198415333873034No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT43670.31593485100709207No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGGAGATT42720.30906198385672023No Hit
CTCCTGCAGGGCCAGTGAAAGAATTATCAGCCACGTGGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT42230.30551703132652847No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG41420.29965700775621146No Hit
CTATTACTGTCAGCAGTATAAGAACTGGCCCATGTGCACTTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAA40010.28945622598565957No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGGAGATTTTGGAGTCTATTACTGTCAGCAGTATAAGAACTGG39370.2848260839054091No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGACACCAGTGGCCCTGGT36770.2660161317043915No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGTCACCCTCTCCTGCAGGGCCAGTGAAAGAATTATCAG35590.25747930724392964No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC35070.2537173168037261No Hit
CAGTAATAGACTCCAAAATCTCCAGACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTCCCAGACCCACTGCCA34420.24901482875347172No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT32660.23628193803278286No Hit
GTCTGTGTCTCCAGGGGAAAGAGTCACCCTCTCCTGCAGGGCCAGTGAAAGAATTATCAGCCACGTGGCCTGGTA32380.23425625087267324No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT32030.23172414192253626No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA31930.23100068222249714No Hit
GTCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACTCTCAC31410.2272386917822936No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT30590.22130632224197266No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC29540.2137099953915617No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG29340.21226307599148345No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT28680.20748824197122512No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC28120.20343686765100594No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG27910.20191760228092373No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG27520.1990961094507711No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCCTGGCCAAAAGTGCACATGGGCCAGTTCTTATACTG27400.19822795781072414No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG27260.19721511423066934No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC26670.19294670200043842No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA26610.19251262618041495No Hit
GCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCATCATCTATCGTGTATCCACCAGGGCCACTGGTGTC26520.19186151245037972No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGACACCA26450.1913550906603523No Hit
GGGGAAAGAGTCACCCTCTCCTGCAGGGCCAGTGAAAGAATTATCAGCCACGTGGCCTGGTACCAGCAGAAAC25250.18267357425988262No Hit
GTCTGGGACACCAGTGGCCCTGGTGGATACACGATAGATGATGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTA25210.182384190379867No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC24890.18006911933974173No Hit
GTAATAGACTCCAAAATCTCCAGACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTCCCAGACCCACTGCCA24460.17695824262957344No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT23240.16813203428909593No Hit
GTCTATTACTGTCAGCAGTATAAGAACTGGCCCATGTGCACTTTTGGCCA23090.16704684473903725No Hit
CTTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCA23070.1669021527990294No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22930.1658893092189746No Hit
CTTCTGGAGAAATGGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGTCACCCTCTC22760.16465942772890807No Hit
GAGTCACCCTCTCCTGCAGGGCCAGTGAAAGAATTATCAGCCACGTGGCCTGGTACCAGCAGAAACCTGGCCAGG22090.15981224773864586No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG21850.15807594445855191No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA21730.15720779281850494No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG21670.15677371699848147No Hit
CTGCTACTCTGGCTCCCAGATACTTCTGGAGAAATGGTGATGACGCAGTC21560.1559779113284384No Hit
GGCCAGTTCTTATACTGCTGACAGTAATAGACTCCAAAATCTCCAGACTGCAGGCTGCTGATGGTGAGAGTGAA21390.15474802983837188No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG21330.15431395401834838No Hit
GAACTGGCCCATGTGCACTTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT21250.15373518625831706No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT21240.15366284028831315No Hit
ATCGTGTATCCACCAGGGCCACTGGTGTCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACTCTCA20780.1503349256681331No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT20660.14946677402808614No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT20600.14903269820806267No Hit
GGCCAAAAGTGCACATGGGCCAGTTCTTATACTGCTGACAGTAATAGACTCCAAAATCTCCAGACTGCAGGCTG20460.14801985462800787No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA20130.1456324376178787No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT20100.145415399707867No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA19540.14136402538764778No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA18540.1341294283872564No Hit
CCAGTGGCCCTGGTGGATACACGATAGATGATGAGCCTGGGAGCCTGGCC18470.133623006597229No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT17690.12798002093692373No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17560.12703952332687285No Hit
CTCCCAGGCTCATCATCTATCGTGTATCCACCAGGGCCACTGGTGTCCCA17550.12696717735686894No Hit
CCCTGTCTGTGTCTCCAGGGGAAAGAGTCACCCTCTCCTGCAGGGCCAGTGAAAGAATTATCAGCCACGTGGC17530.12682248541686109No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA17270.12494149019675935No Hit
GGCCACTGGTGTCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGAGT16790.12146888363657148No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAG16750.12117949975655581No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC16740.1211071537865519No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC16610.12016665617650103No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA16550.11973258035647752No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA16500.11937085050645797No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC16420.11879208274642665No Hit
GCAGAAACCTGGCCAGGCTCCCAGGCTCATCATCTATCGTGTATCCACCAGGGCCACTGGTGTCCCAGACAGGTT16280.11777923916637185No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT16120.11662170364630924No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC15730.1138002108161566No Hit
AGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCT15670.11336613499613311No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC15540.11242563738608222No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACTTCTGGAGAAATGGTGATGACGCAGTCTC15380.11126810186601961No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT15380.11126810186601961No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC15260.11039995022597265No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC15070.10902537679589827No Hit
GCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCCACGTGGCTGATA15060.10895303082589436No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC14710.10642092187575737No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC14700.10634857590575346No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG14670.10613153799574172No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC14550.10526338635569474No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT14180.10258658546554993No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG14170.10251423949554601No Hit
GCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACTTCTGGAGAAATGGTGATGACGCAGTCTCCAGCCACC14160.10244189352554212No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC14060.10171843382550297No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA13850.10019916845542079No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT13830.10005447651541294No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)