FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692541

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692541
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences770400
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT31430.4079698857736241No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25100.3258047767393562No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22170.2877725856697819No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21250.27583073727933544No Hit
GTCTGGGACAGACTTCACTCTCACCATCACCAGCCTAGAGCCTGAAGATT21160.27466251298027No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGACACCA20900.27128764278296985No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCCGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTAATTA19990.2594755970924195No Hit
CTCCTGCAGGGCCAGTCAGAGTATTAATTACTACTTAGCCTGGTACCAACAGAAACATGGCCAGCCTCCCAGGCT17890.23221703011422637No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17860.2318276220145379No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17210.22339044652128764No Hit
GTCTTTGTCTCCCGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTAATTACTACTTAGCCTGGTA16250.2109293873312565No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15750.20443925233644858No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTCTT15660.20327102803738317No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15560.2019730010384216No Hit
GCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTAGCGAC15200.1973001038421599No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14970.1943146417445483No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14500.18821391484942887No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14270.18522845275181724No Hit
GTTTATTACTGTCAGCAGCGTAGCGACTGGCCTCCGATCACCTTCGGCCA14190.18419003115264798No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13770.17873831775700935No Hit
GACTTCACTCTCACCATCACCAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTAGCGACTGG13610.17666147455867082No Hit
CCTTCGGCCAGGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCA12950.1680944963655244No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12690.1647196261682243No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12450.16160436137071651No Hit
TCTATGATGCATCCAAGAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT12000.1557632398753894No Hit
GAGTATTAATTACTACTTAGCCTGGTACCAACAGAAACATGGCCAGCCTCCCAGGCTCCTCATCTATGATGCATC11820.15342679127725856No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCCTGGCCGAAGGTGATCGGAGGCCAGTCGCTACGCTG11770.1527777777777778No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11710.15199896157840082No Hit
CACCATCACCAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTAGCGACTGGCCTCCGATCAC11690.1517393561786085No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11070.14369158878504673No Hit
GTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGGTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA10990.14265316718587748No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10710.13901869158878505No Hit
ATAATAGACAGCCGTGTCTTCAGGTCTCAGGCTGTTCATTTGCAGAAACA10670.13849948078920044No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGA10660.13836967808930425No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10650.1382398753894081No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10550.1369418483904465No Hit
CATCTATGATGCATCCAAGAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA10190.13226895119418486No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCA10190.13226895119418486No Hit
GTATTAATTACTACTTAGCCTGGTACCAACAGAAACATGGCCAGCCTCCC10100.13110072689511942No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10070.13071131879543094No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10040.13032191069574248No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCC9930.12889408099688474No Hit
GTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCCGGGGAAAGAGC9800.1272066458982347No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA9500.12331256490134995No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9230.11980789200415369No Hit
GGCCGAAGGTGATCGGAGGCCAGTCGCTACGCTGCTGACAGTAATAAACT9190.11928868120456905No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG9100.11812045690550363No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC9030.11721183800623054No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA9000.11682242990654204No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA8920.11578400830737279No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8820.11448598130841123No Hit
GCGTAGCGACTGGCCTCCGATCACCTTCGGCCAGGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT8800.1142263759086189No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT8720.11318795430944964No Hit
GTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCACCAGCC8490.110202492211838No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG8480.11007268951194185No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA8380.10877466251298026No Hit
ATCCAAGAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAC8300.107736240913811No Hit
CTCCTGTGCAGCCTCAGGATTCACCTTCACTAGCTATGGCGTCCATTGGGTCCGCCAGGCTCCAGGCAAGGGG8280.1074766355140187No Hit
CTCCCAGGCTCCTCATCTATGATGCATCCAAGAGGGCCACTGGCATCCCA8170.10604880581516095No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT8130.10552959501557632No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA8120.10539979231568017No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC8070.10475077881619939No Hit
GTCTATTATTGTGCGAAAGATGGTGTAGGGACCTTCTTTCCCGACTGGGGCCAGGGAACCCTGGTGACCGTCTC8020.1041017653167186No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT7960.10332294911734165No Hit
CTGCTACTCTGGCTCCCAGACACCACCGGAGAAATTGTGTTGACACAGTC7960.10332294911734165No Hit
GCCTGGTACCAACAGAAACATGGCCAGCCTCCCAGGCTCCTCATCTATGATGCATCCAAGAGGGCCACTGGCATC7880.10228452751817238No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT7850.10189511941848392No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT7730.10033748701973001No Hit
GAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTAATTACTACTTAGCCTGGTACCAACAGAAACATGGCCAGC7720.10020768431983386No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAACG200.00636922253.67332564
GGTATCA7100.048.0845381
GTCGTAT551.4869875E-743.84082439
CGTATGC1200.040.2691841
AACGGCC350.001370607339.29263737
TGAGCGA2800.039.14421531
CACGAGA2900.038.861621
TCGTATG1250.038.62292540
ACGAGAC2800.037.81260722
TCTCCGA2900.037.6644111
CCCACGA3000.037.5564619
CTTATAC2850.037.1347581
TGTCGTA655.557431E-737.0596738
ATGTCGT655.5974306E-737.02575337
GTGGTAT1300.036.7661741
GATGTCG754.177673E-836.637236
CGAGACA2900.036.5158423
ATCTCCG3000.036.4089310
GTATCAA18650.035.8790551
GAGCGAT2600.035.58476332
Produced by FastQC (version 0.11.5)