FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004692542

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692542
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences770400
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT32550.42250778816199375No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22570.2929646936656283No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGACACCA21100.273883696780893No Hit
GTCTGGGACAGACTTCACTCTCACCATCACCAGCCTAGAGCCTGAAGATT20830.2703790238836968No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20770.26960020768431986No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20000.25960539979231567No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC19300.25051921079958467No Hit
CTCCTGCAGGGCCAGTCAGAGTATTAATTACTACTTAGCCTGGTACCAACAGAAACATGGCCAGCCTCCCAGGCT18910.24545690550363447No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCCGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTAATTA18630.24182242990654204No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT18500.24013499480789202No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17450.22650571131879543No Hit
GTCTTTGTCTCCCGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTAATTACTACTTAGCCTGGTA16870.21897715472481827No Hit
GCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTAGCGAC14870.1930166147455867No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14850.19275700934579437No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14410.18704569055036344No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTCTT14240.18483904465212878No Hit
GTTTATTACTGTCAGCAGCGTAGCGACTGGCCTCCGATCACCTTCGGCCA13710.1779595015576324No Hit
GACTTCACTCTCACCATCACCAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTAGCGACTGG13400.1739356178608515No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13090.16991173416407063No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13080.16978193146417445No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12490.16212357217030113No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12430.16134475597092418No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12150.15771028037383178No Hit
GAGTATTAATTACTACTTAGCCTGGTACCAACAGAAACATGGCCAGCCTCCCAGGCTCCTCATCTATGATGCATC11890.15433541017653166No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11830.15355659397715474No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11640.15109034267912774No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11570.15018172377985461No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11560.15005192107995846No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCCTGGCCGAAGGTGATCGGAGGCCAGTCGCTACGCTG11520.14953271028037382No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11380.14771547248182762No Hit
CACCATCACCAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTAGCGACTGGCCTCCGATCAC11010.14291277258566978No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGA10870.14109553478712358No Hit
GGCCGAAGGTGATCGGAGGCCAGTCGCTACGCTGCTGACAGTAATAAACT10570.13720145379023885No Hit
CCTTCGGCCAGGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCA10550.1369418483904465No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCC10450.13564382139148493No Hit
GTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGGTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA10260.13317757009345793No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCA10160.13187954309449637No Hit
TCTATGATGCATCCAAGAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT9890.12837487019730012No Hit
GTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCCGGGGAAAGAGC9370.12162512980269989No Hit
ATAATAGACAGCCGTGTCTTCAGGTCTCAGGCTGTTCATTTGCAGAAACA9350.12136552440290757No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9220.11967808930425754No Hit
GTATTAATTACTACTTAGCCTGGTACCAACAGAAACATGGCCAGCCTCCC9130.1185098650051921No Hit
CATCTATGATGCATCCAAGAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA9080.11786085150571131No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9060.11760124610591902No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT9020.11708203530633438No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8920.11578400830737279No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT8880.11526479750778816No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8770.11383696780893043No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA8760.11370716510903425No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA8710.11305815160955349No Hit
GTCGTGTCCCCTGGCCGAAGGTGATCGGAGGCCAGTCGCTACGCTGCTGA8470.1099428868120457No Hit
CTGCTACTCTGGCTCCCAGACACCACCGGAGAAATTGTGTTGACACAGTC8470.1099428868120457No Hit
CTCCTGTGCAGCCTCAGGATTCACCTTCACTAGCTATGGCGTCCATTGGGTCCGCCAGGCTCCAGGCAAGGGG8470.1099428868120457No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG8380.10877466251298026No Hit
GCGTAGCGACTGGCCTCCGATCACCTTCGGCCAGGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT8330.10812564901349947No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA8280.1074766355140187No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8270.10734683281412252No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8220.10669781931464174No Hit
GGGCTGGAGTGGGTGGCTGTTATGACTTATGATGGAAGTAACAAATACTA8130.10552959501557632No Hit
GTCTATTATTGTGCGAAAGATGGTGTAGGGACCTTCTTTCCCGACTGGGGCCAGGGAACCCTGGTGACCGTCTC7890.10241433021806853No Hit
GTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCACCAGCC7820.10150571131879542No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC7810.10137590861889927No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)