FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692545

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692545
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1265744
Sequences flagged as poor quality0
Sequence length20-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT24350.1923769735428333No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22320.17633897533782503No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19010.15018834772276227No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC18610.14702815103212025No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18050.14260387566522142No Hit
GTAATAGACAGCCGTGTCCTCGTCTGTCAAGTTGTTCATTTGCAGATAGA17080.1349403986904145No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16430.1298050790681212No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT15000.11850737589907596No Hit
GATCTATGCTGCATCCACTTTGCAATCAGGGGTCCCATCTCGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA14960.11819135623001176No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14740.11645324805015865No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14570.11511016445663579No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGCATTTACAATTATTTAGCCTGGTATCAGCAGAAG13690.10815773173722333No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13160.10397047112212264No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13070.10325942686672819No Hit
ACTTATTACTGTCAAACGTCTAACAATGCCCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA12910.10199534819047137No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1050.039.4465141
CGAGACG3650.039.1577723
TACGTAT1204.3458567E-738.23987270
CGTAGAG3750.038.16938828
ACGTAGA3850.038.04960627
AGACGTA3850.038.0210225
ACGAGAC3850.038.00148422
GACGTAG3950.037.07165526
GTATCAA39050.036.8490521
GAGACGT4100.034.8654824
TATACAC4650.034.3836443
CCCACGA4250.033.6110119
TATCAAC42300.033.1319272
ATCAACG43200.032.285473
TCAACGC43800.031.8432034
ATCTCCG4500.031.7399710
CAACGCA43900.031.6931785
AACGCAG44250.031.44256
TCTCCGA4550.030.6437711
CGAGCCC4450.030.56939715
Produced by FastQC (version 0.11.5)