FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692546

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692546
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1265744
Sequences flagged as poor quality0
Sequence length20-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT25400.20067248985576863No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24550.1939570718881543No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18790.14845023954290915No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC18330.14481601334867084No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17650.13944367897457938No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17380.13731054620839603No Hit
GTAATAGACAGCCGTGTCCTCGTCTGTCAAGTTGTTCATTTGCAGATAGA15170.11985045949259882No Hit
GATCTATGCTGCATCCACTTTGCAATCAGGGGTCCCATCTCGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA14520.11471513987030553No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT14440.11408310053217711No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14270.11274001693865425No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13720.10839474648902146No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13660.10792071698542517No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT4100.038.924341
TAACGGC1350.038.10616336
GTATCAA37000.036.433091
TTAACGG1450.035.44984435
TATCAAC40200.032.7585072
ATCAACG41150.032.0834473
TCAACGC41400.031.8127564
CAACGCA41850.031.4719285
AACGCAG42750.030.8105776
AATGGTA9750.029.63401470
ACGGTCA4100.029.49954670
CGGTACC1800.028.9541645
CGCAGAG45650.028.9287878
AGAGTAC46150.028.83605211
ACGCAGA45950.028.7399147
TACGTAT1654.311214E-628.50629470
CAGAGTA47150.028.08046510
GCAGAGT47750.027.7276239
GTGCTAG4300.027.6380141
AATAGAC5800.027.5024343
Produced by FastQC (version 0.11.5)