FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692547

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692547
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1595725
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT59100.3703645678296699No Hit
GACTTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACTAAATGTAAACCA55330.34673894311363174No Hit
GTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCC52840.3311347506619248No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA52700.3302574065080136No Hit
AGCCAGGACAGGCCCCTGTCCTTGTCTTCTATGGTAAAAACAATCGGCCC50170.31440254429804637No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG42400.26570994375597295No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC38330.2402042958529822No Hit
GTCCTAAGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC37550.23531623556690534No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC36750.2303028404016983No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC35790.22428676620344984No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC34960.21908536871954754No Hit
CTATTACTGTAACTCCCGGGACAGCAGTGGTTTACATTTAGTCTTCGGAA32680.20479719249870748No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC31680.19853044854219867No Hit
GTCCTGGCTTCTGCTGGTACCAGCTTCCATAATATTTGCTGAGGCCGTCTCCTTGGCATGTAATCCTGACTGT31430.19696376255307146No Hit
ACTCAGGACCCTGCTGTGTCTGTGGCCCTGGGACAGACAGTCAGGATTAC30150.18894233028874025No Hit
GACTATTACTGTAACTCCCGGGACAGCAGTGGTTTACATTTAGTCTTCGG28710.17991821899136756No Hit
CTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCTGTGTCTGTGGCCCTGGGA28350.1776621911670244No Hit
GGATTACATGCCAAGGAGACGGCCTCAGCAAATATTATGGAAGCTGGTACCAGCAGAAGCCAGGACAGGCCC26910.16863807986965174No Hit
CAGTAATAGTCAGCCTCATCTTCCGCCTGAGCCCCAGTGATGATCAAGGA26750.1676354008366103No Hit
CTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCT26540.16631938460574347No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG25480.15967663601184415No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT25460.15955130113271399No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT25080.15716993842924062No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT25020.1567939337918501No Hit
GTAATAGTCAGCCTCATCTTCCGCCTGAGCCCCAGTGATGATCAAGGAAG24940.1562925942753294No Hit
GCCTTGGGCTGACTTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACTAA24760.1551645803631578No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACTTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACTAAATGTAAAC24360.15265788278055428No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG24310.15234454558272884No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC23510.14733115041752182No Hit
GGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGA23240.14563912954926445No Hit
TCCTAAGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG22370.1401870623071018No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG21610.1354243369001551No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG20730.12990960221842734No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC20640.12934559526234157No Hit
GACAAGGACAGGGGCCTGTCCTGGCTTCTGCTGGTACCAGCTTCCATAAT20290.1271522348775635No Hit
GTCTGTGGCCCTGGGACAGACAGTCAGGATTACATGCCAAGGAGACGGCCTCAGCAAATATTATGGAAGCTGGTA20200.1265882279214777No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG19250.12063482116279434No Hit
GGACAGGGGCCTGTCCTGGCTTCTGCTGGTACCAGCTTCCATAATATTTG19000.11906813517366713No Hit
TCACTGGGGCTCAGGCGGAAGATGAGGCTGACTATTACTGTAACTCCCGG18460.11568409343715239No Hit
ATGTAAACCACTGCTGTCCCGGGAGTTACAGTAATAGTCAGCCTCATCTT18170.11386673768976484No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG17570.11010669131585955No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA17450.1093546820410785No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA17130.10734932397499569No Hit
CCCCTGTCCTTGTCTTCTATGGTAAAAACAATCGGCCCTCAGGGATCCCA17070.10697331933760518No Hit
ACCCTGCTGTGTCTGTGGCCCTGGGACAGACAGTCAGGATTACATGCCAA17070.10697331933760518No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT16860.10565730310673832No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA16810.10534396590891287No Hit
GTCCTTGTCTTCTATGGTAAAAACAATCGGCCCTCAGGGATCCCAGACCG16580.10390261479891584No Hit
GGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCTGTGTCTGTGG16580.10390261479891584No Hit
CTTCTGAGCTGACTCAGGACCCTGCTGTGTCTGTGGCCCTGGGACAGACA16210.10158391953500759No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC16100.10089457769979163No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC16020.10039323818327092No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG1550.053.3376840
ATTCGCA606.140775E-446.25478770
CGTATGC1800.045.99228741
TCTCGTA1500.045.8165238
ATCTCGT1650.045.77892737
CGAGACG2100.040.59731323
ATCTCCG2200.040.2894310
TACGTAT700.001311457839.6469670
CTAACGC451.07380474E-437.88373
ACGAGAC2350.036.2773122
TCGGTAT400.002148104835.85965763
CACGAGA2400.035.5193121
GTATCAA30950.035.3666341
TATACAC2700.035.358123
CTTATAC2650.034.7429921
TTCGATC601.369713E-534.40041739
CCTAACG601.4558687E-534.0953332
CCCACGA2600.034.0942619
GTATGCC2450.033.83423242
ATGCCGT2250.033.83064344
Produced by FastQC (version 0.11.5)