FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692548

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692548
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1595725
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT53870.3375894969371289No Hit
GTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCC52750.33057074370583905No Hit
GACTTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACTAAATGTAAACCA49130.3078851305832772No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA49050.3073837910667565No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC44810.28081279669115916No Hit
AGCCAGGACAGGCCCCTGTCCTTGTCTTCTATGGTAAAAACAATCGGCCC44670.27993545253724794No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC39460.24728571652383713No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG39040.24465368406210342No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC38980.24427767942471293No Hit
GTCCTAAGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC35310.2212787291043256No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC33870.21225461780695296No Hit
CTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCTGTGTCTGTGGCCCTGGGA31490.197339767190462No Hit
CTATTACTGTAACTCCCGGGACAGCAGTGGTTTACATTTAGTCTTCGGAA30630.19195036738786445No Hit
GTCCTGGCTTCTGCTGGTACCAGCTTCCATAATATTTGCTGAGGCCGTCTCCTTGGCATGTAATCCTGACTGT28910.18117156778266932No Hit
CTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCT28530.17879020507919596No Hit
ACTCAGGACCCTGCTGTGTCTGTGGCCCTGGGACAGACAGTCAGGATTAC28170.17653417725485282No Hit
GGATTACATGCCAAGGAGACGGCCTCAGCAAATATTATGGAAGCTGGTACCAGCAGAAGCCAGGACAGGCCC27790.17415281455137946No Hit
GACTATTACTGTAACTCCCGGGACAGCAGTGGTTTACATTTAGTCTTCGG27730.17377680991398894No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG27700.1735888075952937No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACTTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACTAAATGTAAAC26230.16437669397922575No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC26160.1639380219022701No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT25360.1589246267370631No Hit
GGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGA24700.1547885757257673No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT24600.15416190133011642No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG24590.15409923389055133No Hit
GCCTTGGGCTGACTTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACTAA23650.14820849457143304No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG23640.14814582713186794No Hit
CAGTAATAGTCAGCCTCATCTTCCGCCTGAGCCCCAGTGATGATCAAGGA23120.1448871202744834No Hit
GTAATAGTCAGCCTCATCTTCCGCCTGAGCCCCAGTGATGATCAAGGAAG22210.13918438327406038No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT22140.13874571119710477No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC21560.13511099970232968No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG21290.1334189788340723No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC20960.1313509533284244No Hit
GTCTGTGGCCCTGGGACAGACAGTCAGGATTACATGCCAAGGAGACGGCCTCAGCAAATATTATGGAAGCTGGTA20570.12890692318538596No Hit
TCCTAAGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG19780.12395619545974401No Hit
GACAAGGACAGGGGCCTGTCCTGGCTTCTGCTGGTACCAGCTTCCATAAT19710.1235175233827884No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT19700.1234548559432233No Hit
GTCCTTGTCTTCTATGGTAAAAACAATCGGCCCTCAGGGATCCCAGACCG19420.12170016763540083No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA19090.11963214212975294No Hit
GGACAGGGGCCTGTCCTGGCTTCTGCTGGTACCAGCTTCCATAATATTTG18520.11606009807454293No Hit
GGTTTACATTTAGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAAGTCAGCCCAAGGCCAACCCCACTGTCACT18350.11499475160193642No Hit
GGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCTGTGTCTGTGG18320.11480674928324117No Hit
TCACTGGGGCTCAGGCGGAAGATGAGGCTGACTATTACTGTAACTCCCGG18020.11292672609628852No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17770.11136004010716131No Hit
ATGTAAACCACTGCTGTCCCGGGAGTTACAGTAATAGTCAGCCTCATCTT17600.11029469363455482No Hit
CCCCTGTCCTTGTCTTCTATGGTAAAAACAATCGGCCCTCAGGGATCCCA17480.10954268435977377No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC17260.10816400068934184No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT16450.10308793808456972No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA16430.10296260320543954No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT16370.10258659856804901No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC16320.10227326137022356No Hit
CTTCTGAGCTGACTCAGGACCCTGCTGTGTCTGTGGCCCTGGGACAGACA16110.10095724513935671No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT16090.10083191026022653No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC16030.10045590562283602No Hit
ACCCTGCTGTGTCTGTGGCCCTGGGACAGACAGTCAGGATTACATGCCAA15970.10007990098544549No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC15960.10001723354588041No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)