FastQCFastQC Report
Wed 8 May 2024
EGAF00004692551

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692551
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1750833
Total Bases129.1 Mbp
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT86010.4912518783915999No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC83690.47800104293213574No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC59410.33932419596843333No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC58300.3329843565891207No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC54040.30865308113338047No Hit
GAGTTACAGTCCCTCTTGGACGTTCGGCCAAGGGACCAGGGTGGAAATCA53000.3027130514446552No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG51070.291689727118463No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA49980.28546411907931823No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT48170.27512618279413287No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG47910.27364117537195154No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG45190.2581057131091315No Hit
GACTATTAGTGTCTATTTAAATTGGTATCAGCAGAAACCAGGAAAAGCCC45110.2576487877484603No Hit
CTCTTGGACGTTCGGCCAAGGGACCAGGGTGGAAATCAAACGAACTGTGG44430.25376492218275526No Hit
GTCTCCATCCTCCCTATCTGCATCCGTAGGAGACCGAGTCACCATCACTT43450.24816758651453338No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG42780.24434083661891226No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT40800.23303193394230062No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA40400.23074730713894473No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTC38290.2186959007512424No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT38140.21783916569998396No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG37780.21578300157696365No Hit
GAGTTCTCTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAAGTTA37470.21401241580436284No Hit
GTCCAAGAGGGACTGTAACTCTGTTGACAGTAGTAACTTGCAAAATCTTC36970.21115663230016796No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT36250.20704430405412738No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCCTGGTCCCTTGGCCGAACGTC36040.20584487498236553No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA35960.20538794962169435No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGGCCCAGTC35190.20099004302523427No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG34860.19910522591246568No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC33020.18859594261702858No Hit
CTAATAGTCTGACTTGCCCGACAAGTGATGGTGACTCGGTCTCCTACGGA32100.18334130096931003No Hit
CTGTAACTCTGTTGACAGTAGTAACTTGCAAAATCTTCAGGTTGCAGACT31750.18134225251637365No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCCTGGTCC30530.17437414076613816No Hit
CTCCTGATCTATGCTTCATCCAATTTGCAAAGTGGGGTCCCGTCAAGGTT30470.17403144674563478No Hit
GATCTATGCTTCATCCAATTTGCAAAGTGGGGTCCCGTCAAGGTTCAGTG29100.16620659994414086No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCT28600.163350816439946No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT28310.16169446200751297No Hit
CTATTAGTGTCTATTTAAATTGGTATCAGCAGAAACCAGGAAAAGCCCCT28300.1616373463374291No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG28180.16095195829642234No Hit
ATCTGGGACAGAGTTCTCTCTCACCATCAGCAGTCTGCAACCTGAAGATT27450.15678251438029783No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGC26930.15381249953593518No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCT26880.1535269211855157No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT26190.1495859399497268No Hit
GATCAGGAGTTCAGGGGCTTTTCCTGGTTTCTGCTGATACCAATTTAAAT26090.1490147832488878No Hit
AGTTACTACTGTCAACAGAGTTACAGTCCCTCTTGGACGTTCGGCCAAGG26070.14890055190872No Hit
GTTTATTACTGTGCGAGGGATACGCGTTTAGCAGCAGCTGCTAACTTTCA25710.14684438778569972No Hit
GTAATAAACAGCCGTGTCCCCAGATCTCAGGCTGCTCATGTCCATGTAGG25540.14587342139427348No Hit
GTCCCAGATCCACTGCCACTGAACCTTGACGGGACCCCACTTTGCAAATT25100.143360331910582No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25010.14284629087982692No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG24940.14244648118923964No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA24460.1397049290252126No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA24360.1391337723243736No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC23330.1332508583057322No Hit
GTAGTAACTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGAGA23300.13307951129548048No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC23140.13216566057413814No Hit
GTAGGAGACCGAGTCACCATCACTTGTCGGGCAAGTCAGACTATTAGTGT22950.1310804628425441No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA22680.12953833975027887No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC22540.1287387203691043No Hit
GTTACAGTCCCTCTTGGACGTTCGGCCAAGGGACCAGGGTGGAAATCAAA22430.12811044799818144No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA22410.12799621665801364No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT22400.12793910098792974No Hit
CCCTATCTGCATCCGTAGGAGACCGAGTCACCATCACTTGTCGGGCAAGT22080.12611139954524503No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC22020.12576870552474168No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCC21530.1229700376906307No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC21250.12137079892828156No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC21080.12039983253685532No Hit
GTTCAGTGGCAGTGGATCTGGGACAGAGTTCTCTCTCACCATCAGCAGTC21060.12028560119668752No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAG21030.12011425418643584No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC20960.11971444449584855No Hit
GTCTGCAACCTGAAGATTTTGCAAGTTACTACTGTCAACAGAGTTACAGT20820.11891482511467398No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC20580.11754404903266044No Hit
CCATCAGCAGTCTGCAACCTGAAGATTTTGCAAGTTACTACTGTCAACAG20340.11617327295064693No Hit
GAGAGAACTCTGTCCCAGATCCACTGCCACTGAACCTTGACGGGACCCCA20090.1147453811985495No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCA19980.11411710882762661No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGT19900.11366018346695544No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC19770.11291767975586478No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT19690.1124607543951936No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG19620.11206094470460631No Hit
GTGGGGTCCCGTCAAGGTTCAGTGGCAGTGGATCTGGGACAGAGTTCTCT19570.11177536635418683No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT19550.11166113501401904No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC19380.11069016862259279No Hit
GCCCAGTCTCCATCCTCCCTATCTGCATCCGTAGGAGACCGAGTCACCAT19360.110575937282425No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA19260.11000478058158603No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC19010.1085768888294886No Hit
GCATAGATCAGGAGTTCAGGGGCTTTTCCTGGTTTCTGCTGATACCAATT18900.10794861645856574No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA18880.10783438511839792No Hit
GACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCG18610.1062922620261327No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACT18390.10503571728428696No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT18320.10463590759369969No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA18320.10463590759369969No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG18310.1045787919236158No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC17950.10252262780059548No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC17760.10143743006900145No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG17750.10138031439891754No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)