FastQCFastQC Report
Wed 8 May 2024
EGAF00004692552

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692552
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1750833
Total Bases128.9 Mbp
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT84780.48422665097128054No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC73730.4211138355285742No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC60550.3458353823579976No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC55120.3148215735024414No Hit
GAGTTACAGTCCCTCTTGGACGTTCGGCCAAGGGACCAGGGTGGAAATCA52520.2999714992806281No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC50910.2907758763971207No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT49690.28380776464688523No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG49450.2824369885648717No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA46990.268386533724233No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG44110.2519372207400706No Hit
GTCTCCATCCTCCCTATCTGCATCCGTAGGAGACCGAGTCACCATCACTT42510.24279871352664703No Hit
CTCTTGGACGTTCGGCCAAGGGACCAGGGTGGAAATCAAACGAACTGTGG42240.2412565904343818No Hit
GACTATTAGTGTCTATTTAAATTGGTATCAGCAGAAACCAGGAAAAGCCC41880.2392004263113615No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT40040.22869114301592441No Hit
GAGTTCTCTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAAGTTA39300.22446458342971604No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG39080.22320803868787026No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA38930.22235130363661182No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT38630.2206378335340949No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT38270.2185816694110746No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37670.21515472920604078No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCCTGGTCCCTTGGCCGAACGTC37150.21218471436167813No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC36930.2109281696198324No Hit
GTCCAAGAGGGACTGTAACTCTGTTGACAGTAGTAACTTGCAAAATCTTC36380.20778680776521805No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA36220.2068729570438757No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG34750.19847695354154282No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTC34320.1960209797279352No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG34300.19590674838776742No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGC33610.1919657671519785No Hit
CTCCTGATCTATGCTTCATCCAATTTGCAAAGTGGGGTCCCGTCAAGGTT33010.18853882694694468No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGGCCCAGTC32680.18665400983417607No Hit
GATCTATGCTTCATCCAATTTGCAAAGTGGGGTCCCGTCAAGGTTCAGTG31100.17762973396092033No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG30670.17517376014731273No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT30650.17505952880714495No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG30300.17306048035420854No Hit
CTAATAGTCTGACTTGCCCGACAAGTGATGGTGACTCGGTCTCCTACGGA29640.16929084612867132No Hit
CTGTAACTCTGTTGACAGTAGTAACTTGCAAAATCTTCAGGTTGCAGACT29620.16917661478850354No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCCTGGTCC29170.16660640963472817No Hit
GTGGGGTCCCGTCAAGGTTCAGTGGCAGTGGATCTGGGACAGAGTTCTCT27340.15615424200937497No Hit
CTATTAGTGTCTATTTAAATTGGTATCAGCAGAAACCAGGAAAAGCCCCT27170.1551832756179487No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG26680.15238460778383775No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCT26420.15089960036165642No Hit
GATCAGGAGTTCAGGGGCTTTTCCTGGTTTCTGCTGATACCAATTTAAAT26070.14890055190872No Hit
GTTTATTACTGTGCGAGGGATACGCGTTTAGCAGCAGCTGCTAACTTTCA25800.1473584288164548No Hit
GTCCCAGATCCACTGCCACTGAACCTTGACGGGACCCCACTTTGCAAATT25360.14484533933276333No Hit
ATCTGGGACAGAGTTCTCTCTCACCATCAGCAGTCTGCAACCTGAAGATT25310.14455976098234383No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCT24830.14181820881831675No Hit
GTAATAAACAGCCGTGTCCCCAGATCTCAGGCTGCTCATGTCCATGTAGG24680.1409614737670583No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA24630.14067589541663883No Hit
AGTTACTACTGTCAACAGAGTTACAGTCCCTCTTGGACGTTCGGCCAAGG24290.13873396263378632No Hit
GTAGTAACTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGAGA23410.13370778366640337No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAA23050.13165161954338306No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT22970.1311946941827119No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA22240.1270252502665874No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGC22230.1269681345965035No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA22100.12622563088541283No Hit
GTAGGAGACCGAGTCACCATCACTTGTCGGGCAAGTCAGACTATTAGTGT21870.12491197047348318No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAG21860.12485485480339929No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC21710.12399811975214084No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC21690.12388388841197305No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCC21640.12359831006155356No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCA20950.11965732882576466No Hit
GTTCAGTGGCAGTGGATCTGGGACAGAGTTCTCTCTCACCATCAGCAGTC20820.11891482511467398No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA20600.11765828037282824No Hit
CCCTATCTGCATCCGTAGGAGACCGAGTCACCATCACTTGTCGGGCAAGT20330.11611615728056301No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC20310.11600192594039523No Hit
GTCTGCAACCTGAAGATTTTGCAAGTTACTACTGTCAACAGAGTTACAGT20210.11543076923955625No Hit
GTTACAGTCCCTCTTGGACGTTCGGCCAAGGGACCAGGGTGGAAATCAAA20200.11537365356947236No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC19960.11400287748745883No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC19840.11331748944645208No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC19640.1121751760447741No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGT19450.11108997831318007No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC19360.110575937282425No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC19270.11006189625166991No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC19230.10983343357133432No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18850.10766303810814624No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC18720.10692053439705558No Hit
GAGAGAACTCTGTCCCAGATCCACTGCCACTGAACCTTGACGGGACCCCA18710.10686341872697168No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC18150.10366494120227343No Hit
GCATAGATCAGGAGTTCAGGGGCTTTTCCTGGTTTCTGCTGATACCAATT18090.10332224718177005No Hit
GCCCAGTCTCCATCCTCCCTATCTGCATCCGTAGGAGACCGAGTCACCAT18070.10320801584160226No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT17750.10138031439891754No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT17670.10092338903824637No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA17660.10086627336816245No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT17560.10029511666732349No Hit
GGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG17530.10012376965707181No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT17510.100009538316904No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)