FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692555

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692555
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1526905
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC56010.36682046361757936No Hit
GACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACCCAAGTGGTGGCGCTGGAATATGAGCTGCAGTAATAAT53340.3493341105045828No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGCCAGTCGATCACCATCTCCTG44860.293796929082032No Hit
GCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACCCAAGTGGTGGCGCTGGAATATGAGCTG43920.2876406849149096No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG41950.2747387689476425No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT40390.26452202330858826No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG40040.26222980473572355No Hit
GATTATTACTGCAGCTCATATTCCAGCGCCACCACTTGGGTGTTCGGCGGAGGGACCAAGGTGACCGTCCTAGGT34330.22483389601841633No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC32180.2107531247851045No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTCGTTATAACCATGTCTGCTGGTTCCAGCAGCACCCAGGCAAAGT32120.21036017302975626No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA32110.21029468107053154No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATAT31090.2036145012296115No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC31040.20328704143348802No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTC29790.19510054653039974No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT29610.19392169126435502No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG29460.19293931187598443No Hit
TTATAACCATGTCTGCTGGTTCCAGCAGCACCCAGGCAAAGTCCCCAAAC28770.1884203666894797No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG28730.18815839885258087No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG28250.185014784809795No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT27980.18324650191072792No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG27310.1788585406426726No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC25590.16759392365602313No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG25460.16674252818610194No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC25380.1662185925123043No Hit
TCCTCACCCTCCTCACTCCGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGT25290.16562916487928195No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG25040.1639918658986643No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG24580.1609792357743278No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG24340.15940742875293487No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA23580.1544300398518572No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG23380.15312020066736307No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA22800.1493216670323301No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT22610.14807731980706068No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGCCAGTCGATCACCATCT22010.14414780225357832No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC21910.14349288266133126No Hit
ACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACCCAAGTGGTGGCGC21560.14120066408846654No Hit
GTAGTAGAGGGCTGTGTCCTCGGTTTTCAGGCTGTTCATTTGCAGATATA21520.1409386962515677No Hit
CCCAAGTGGTGGCGCTGGAATATGAGCTGCAGTAATAATCAGCCTCGTCC20900.13687819477963592No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA20490.13419302445142298No Hit
CCTTGGTCCCTCCGCCGAACACCCAAGTGGTGGCGCTGGAATATGAGCTGCAGTAATAATCAGCCTCGTCCTCAG19620.12849522399887353No Hit
GGCCTGGGCTCTGCTCTTCCTCACCCTCCTCACTCCGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC19540.1279712883250759No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA19380.12692341697748058No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTCGTTATAACCATGTCTGCTGGTTCCAGCAGCA18260.11958831754431348No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGCCAGTCGATCAC18180.11906438187051585No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA18070.11834397031904409No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT17820.11670667133842642No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC17820.11670667133842642No Hit
TGGTTATAACGACCAACGTCACTGCTGGTTCCAGTGCAGGAGATGGTGAT17780.1164447035015276No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT17730.11611724370540408No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC17460.114348960806337No Hit
CCACTTGGGTGTTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAGCCC17310.1133665814179664No Hit
CTCCTGGCCAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTT16960.11107436284510169No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTCGCCAGACTTGGAGCCAGAGAACCGATTAGAAACCCCTGAGGGCCGA16860.11041944325285463No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACAC16820.1101574754159558No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT16790.10996099953828169No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT16370.10721033725084403No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA16220.10622795786247345No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC950.047.23649241
GGTATCA10950.046.023171
TCGTATG1100.043.86624540
GGATGGG10350.039.9182470
GTATCAA32400.039.726081
ATCTCGT1009.094947E-1237.75900337
GTGGTAT2700.036.573221
TATCAAC36250.035.4094852
ATCAACG37900.034.0475853
TCAACGC38150.033.8244674
CAACGCA39200.032.9184535
AACGCAG39500.032.754646
CGAGACA2050.031.562523
AATCTCG1101.1259544E-931.18725436
TGCCGTC1600.030.3775845
ACGCAGA42550.030.3267547
CGCAGAG42600.030.2891758
AGAGTAC43250.030.07011411
CAGAGTA43600.029.82872610
GCAGAGT43800.029.6147929
Produced by FastQC (version 0.11.5)