FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692556

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692556
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1526905
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC55800.3654451324738605No Hit
GACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACCCAAGTGGTGGCGCTGGAATATGAGCTGCAGTAATAAT48750.3192733012204426No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGCCAGTCGATCACCATCTCCTG46620.30532351390558027No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG46600.3051925299871308No Hit
GCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACCCAAGTGGTGGCGCTGGAATATGAGCTG46270.30303129533271556No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG38130.2497208405238047No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT36650.24002803055854816No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC33780.22123183826105752No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTCGTTATAACCATGTCTGCTGGTTCCAGCAGCACCCAGGCAAAGT32320.2116700122142504No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG32310.21160452025502569No Hit
GATTATTACTGCAGCTCATATTCCAGCGCCACCACTTGGGTGTTCGGCGGAGGGACCAAGGTGACCGTCCTAGGT32180.2107531247851045No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG31590.20688909919084683No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG31220.20446589669953272No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATAT30060.19686882942946682No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC29410.1926118520798609No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT28630.1875034792603338No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT28120.18416338933987378No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG28100.1840324054214244No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTC28060.18377043758452558No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA27900.18272256623693026No Hit
TTATAACCATGTCTGCTGGTTCCAGCAGCACCCAGGCAAAGTCCCCAAAC26100.17093401357648313No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG25540.1672664638598996No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC25470.16680802014532667No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA25310.16576014879773135No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA25210.16510522920548432No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG24900.1630749784695184No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG24590.1610447277335525No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG24220.15862152524223838No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG23350.15292372478968896No Hit
TCCTCACCCTCCTCACTCCGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGT23000.15063150621682425No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACAC22340.1463090369079936No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC21660.1418555836807136No Hit
GGCCTGGGCTCTGCTCTTCCTCACCCTCCTCACTCCGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC20720.13569933951359123No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGCCAGTCGATCACCATCT20230.13249023351158062No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT20220.13242474155235592No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC19960.13072195061251354No Hit
CCCAAGTGGTGGCGCTGGAATATGAGCTGCAGTAATAATCAGCCTCGTCC19930.13052547473483944No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19700.1290191596726712No Hit
GTAGTAGAGGGCTGTGTCCTCGGTTTTCAGGCTGTTCATTTGCAGATATA19670.12882268379499706No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGCCAGTCGATCAC19500.12770932048817707No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA19450.1273818606920535No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC19360.12679243305903118No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA19020.12456570644539117No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT18200.11919536578896527No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT18150.11886790599284172No Hit
ACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACCCAAGTGGTGGCGC17930.11742708288989818No Hit
CCTTGGTCCCTCCGCCGAACACCCAAGTGGTGGCGCTGGAATATGAGCTGCAGTAATAATCAGCCTCGTCCTCAG17540.11487289648013466No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC17540.11487289648013466No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA17540.11487289648013466No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTCGCCAGACTTGGAGCCAGAGAACCGATTAGAAACCCCTGAGGGCCGA17480.11447994472478641No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC17460.114348960806337No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA17400.11395600905098878No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTCGTTATAACCATGTCTGCTGGTTCCAGCAGCA17350.11362854925486524No Hit
CTCCTGGCCAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTT17220.11277715378494406No Hit
CCACTTGGGTGTTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAGCCC16720.10950255582370876No Hit
TGGTTATAACGACCAACGTCACTGCTGGTTCCAGTGCAGGAGATGGTGAT16620.10884763623146168No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT15920.10426319908573224No Hit
CTCCGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCC15450.10118507700217105No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC15390.10079212524682281No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT15360.1005956493691487No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTCA350.003745936361.3758470
AACGCGC200.007344166751.77725641
GATATAC200.007773163751.0390851
GGATGGG13900.043.7873270
GTATCAA32600.038.0966571
GGTATCA13000.037.428661
AATGGTA5300.036.47809670
GTGGTAT4900.034.0260541
TATCAAC37400.032.8271942
AATAGCG802.028146E-631.407462
ATCAACG39200.031.0524273
TCAACGC39450.030.7734854
ACGAACC802.419094E-630.70568352
TGGTATC5450.030.5772022
CAACGCA39950.030.3893325
AACGCAG40250.030.3337966
GTCATAG10600.030.174051
TCAGGTC10850.030.0892412
AGGATGG13150.029.82777269
ATAGCGG853.1755808E-629.65217263
Produced by FastQC (version 0.11.5)