FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692559

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692559
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1233176
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATACTAGTGATGTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT84490.6851414558830208No Hit
CTAGTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCCGATGGTGAGAGTGAATTCTGTC58500.4743848404445108No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT57910.4696004463272072No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC56010.4541930754409752No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC45770.37115545550675655No Hit
CAGTATACTAGTGATGTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT36400.2951727896099178No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC36250.29395641822416263No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG35320.28641491563248067No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC35130.2848741785438575No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG34620.2807385158322899No Hit
GAATTCACTCTCACCATCGGCAGCCTGCAGCCTGATGATTTTGCAACTTA33160.26889916767760647No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA33080.26825043627187034No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT31650.25665436239433786No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG30380.24635575132827756No Hit
GTTGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAATATTTATAGCTGGTTGGCCTGGTATCAGCAGAAA30250.24530156279395643No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC30030.24351755142818218No Hit
GTCTGCATCTGTTGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA29420.23857097445944458No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG28060.22754254056193113No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT27910.22632616917617598No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG27490.22292032929606154No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT27390.22210941503889142No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG26280.21310826678430328No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT25670.20816168981556568No Hit
AGTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGC25160.20402602710399811No Hit
ATTTATAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAA24610.19956599868956254No Hit
ATACTAGTGATGTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA24420.19802526160093936No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22840.18521281633765171No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG22570.18302334784329244No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT21440.17386001673727025No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT20920.16964326259998574No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20250.16421013707694604No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19810.16064211434539757No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT19810.16064211434539757No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19400.15731736589100015No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG19140.1552089888223579No Hit
GAGTGAATTCTGTCCCAGAACGACTGCCGCTGAATCTTGGTGGGACCCCA19030.15431698313947076No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG18930.15350606888230067No Hit
CTTCCACCCTGTCTGCATCTGTTGGAGACAGAGTCACCATCACTTGCCGG18480.14985695472503519No Hit
GAATATTTATAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAACCTCCTGATCTATGAGGCGT18230.14782966908210993No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTTTCCTTCCACC17750.14393728064769346No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG17630.14296418353908932No Hit
ACTTATTACTGCCAACAGTATACTAGTGATGTCACTTTCGGCGGAGGGAC17460.14158562930190013No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC17070.13842306369893673No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC17050.1382608808475027No Hit
CACTAGTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCCGATGGTGAGAGTGAATT17030.1380986979960687No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT17020.13801760657035167No Hit
GGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGCTATAA16780.13607141235314343No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT16720.13558486379884135No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC16630.13485504096738826No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC16450.1333953953044821No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC16260.13185465821585887No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT16040.13007064685008465No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA15760.12780008693000836No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG15530.12593498413851711No Hit
ATCTTGGTGGGACCCCACTTTTTAAAGTAGACGCCTCATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTG15070.12220477855553465No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15020.12179932142694959No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14980.12147495572408155No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT14950.12123168144693053No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT14930.1210694985954965No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG14830.1202585843383264No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA14790.11993421863545836No Hit
GCTATAAATATTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCAACAGATGCAGACAGGGTGGAAGGAAA14780.11985312720974135No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT14700.11920439580400527No Hit
GATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACC14700.11920439580400527No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14660.11888003010113724No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14520.11774475014109907No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC14380.11660947018106094No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC14320.11612292162675886No Hit
GCCCCTAACCTCCTGATCTATGAGGCGTCTACTTTAAAAAGTGGGGTCCCACCAAGATTCAGCGGCAGTCGTT14120.11450109311241866No Hit
GGTATCAGCAGAAACCAGGGAAAGCCCCTAACCTCCTGATCTATGAGGCGTCTACTTTAAAAAGTGGGGTCCCAC14110.11442000168670166No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC13900.11271708174664444No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA13600.11028433897513412No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC13550.10987888184654908No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG13490.10939233329224701No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA13480.10931124186652999No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG13400.10866251046079392No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT13320.10801377905505782No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC13300.10785159620362382No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC13120.10639195054071764No Hit
CACCAAGATTCAGCGGCAGTCGTTCTGGGACAGAATTCACTCTCACCATCGGCAGCCTGCAGCCTGATGATTTTG13120.10639195054071764No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC12630.10241847068058411No Hit
GTCTCCAACAGATGCAGACAGGGTGGAAGGAAACTGGGTCATCTGGATGTCACATTTGGCACCTGGGAGCCAGAG12420.1007155507405269No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT12400.10055336788909287No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)