FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692563

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692563
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1527658
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGCTAGCACACCGTACACTTTTGGCCAGGGGA47770.31270087938530744No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT38430.25156154060660174No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCACTCTGTTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA35250.23074536316374478No Hit
CTCCTGTAGGGTCAGTGAGAGTCTTGACAACAACTACTTAGCCTGGTACC33410.21870078250498473No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC32930.2155587179853082No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28580.18708375827573973No Hit
GTGTATTACTGTGCGAGAGATATTTATGCTGGTAACCCCGGACCGTGGGGCCAGGGAACCCTGGTCACCGTCTC28310.18531634698342167No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA26870.1758901534243921No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24860.16273275824824665No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24720.16181632276334101No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG23490.1537647824316699No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA22740.14885530661967536No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT21540.14100014532048402No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21350.13975641144811207No Hit
GTCTGGGACAGACTTCACTCTCACCATCAACAGAGTGGAGCCTGAAGATT21330.13962549209312555No Hit
GTCTGGGGCCGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAA20950.13713802434838163No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGTAGGGTCAGTGAGAGTCTTGACAA20820.13628704854096924No Hit
GTGTATTACTGTCAGCAGTATGCTAGCACACCGTACACTTTTGGCCAGGGGACCAAGTTGGAGATCAAACGAA20810.136221588863476No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT20490.13412687918369165No Hit
CCTGTAGGGTCAGTGAGAGTCTTGACAACAACTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGG20430.1337341211187321No Hit
GTGCTAGCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCACTCTGTTGATGGTGAGAGTGAAGT20320.1330140646663062No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG20260.13262130660134663No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20240.13249038724636011No Hit
GTAATACACGGCCGTGTCCTCGGATCTCAGGCTGCTCAGCTCCATGTAGG20230.13242492756886684No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20140.13183579047142752No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC20060.1313121130514814No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19880.1301338388566027No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19620.12843188724177793No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC18690.12234413723490468No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT18630.1219513791699451No Hit
GTAATACACTGCAAAATCTTCAGGCTCCACTCTGTTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA17670.11566725013059205No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17370.11370345980579423No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGTAGGGTCAGTGAGA17240.11285248399838183No Hit
GGCCAAAAGTGTACGGTGTGCTAGCATACTGCTGACAGTAATACACTGCA17010.11134691141603684No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG16980.11115053238355706No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG16830.11016863722115813No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGT16800.10997225818867835No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG16740.1095795001237188No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16680.10918674205875921No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16580.10853214528382663No Hit
CTTTTGGCCAGGGGACCAAGTTGGAGATCAAACGAACTGTGGCTGCACCA16370.10715749205646814No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT16160.10578283882910966No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC16110.10545554044164335No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG16040.10499732269919054No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAACTTGGTCCCCTGGCCAAAAGTG15840.1036881291493253No Hit
CAGTAATACACGGCCGTGTCCTCGGATCTCAGGCTGCTCAGCTCCATGTA15700.10277169366441966No Hit
ATGCTAGCACACCGTACACTTTTGGCCAGGGGACCAAGTTGGAGATCAAA15360.10054606462964878No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC15350.10048060495215552No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15320.10028422591967574No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)