FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692564

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692564
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1527658
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGCTAGCACACCGTACACTTTTGGCCAGGGGA43090.2820657503184613No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT38330.2509069438316691No Hit
CTCCTGTAGGGTCAGTGAGAGTCTTGACAACAACTACTTAGCCTGGTACC35470.23218547606859652No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCACTCTGTTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA33060.21640969379272063No Hit
GTGTATTACTGTGCGAGAGATATTTATGCTGGTAACCCCGGACCGTGGGGCCAGGGAACCCTGGTCACCGTCTC29260.19153501634528147No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC27640.1809305485913732No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27520.18014503246145408No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA26640.1743845808420471No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24630.16122718566590166No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22950.15022995984703383No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22670.1483970888772225No Hit
GTCTGGGACAGACTTCACTCTCACCATCAACAGAGTGGAGCCTGAAGATT22520.14741519371482362No Hit
GTGTATTACTGTCAGCAGTATGCTAGCACACCGTACACTTTTGGCCAGGGGACCAAGTTGGAGATCAAACGAA21640.14165474209541665No Hit
GTGCTAGCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCACTCTGTTGATGGTGAGAGTGAAGT20970.13726894370336817No Hit
GTAATACACGGCCGTGTCCTCGGATCTCAGGCTGCTCAGCTCCATGTAGG20800.13615612918598272No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20670.13530515337857035No Hit
CCTGTAGGGTCAGTGAGAGTCTTGACAACAACTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGG20620.13497785499110404No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT20250.1325558469238534No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC20220.1323594678913736No Hit
GTCTGGGGCCGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAA20150.13190125014892076No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20150.13190125014892076No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGTAGGGTCAGTGAGAGTCTTGACAA20010.13098481466401513No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT19690.12889010498423076No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA19430.12718815336940598No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18730.12260597594487771No Hit
GTAATACACTGCAAAATCTTCAGGCTCCACTCTGTTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA18570.12155862110498554No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG18470.12090402433005293No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18190.11907115336024163No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG18050.11815471787533598No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG17290.11317978238584814No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT17250.1129179436758751No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC17080.11180512915848966No Hit
GGCCAAAAGTGTACGGTGTGCTAGCATACTGCTGACAGTAATACACTGCA17050.11160875012600988No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGT17020.1114123710935301No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC16780.10984133883369183No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAACTTGGTCCCCTGGCCAAAAGTG16740.1095795001237188No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGTAGGGTCAGTGAGA16690.1092522017362525No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16630.10885944367129292No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16590.10859760496131987No Hit
GTGTGCTAGCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCACTCTGTTGATGGTGAGAGTGAAGT16550.10833576625134685No Hit
CTTTTGGCCAGGGGACCAAGTTGGAGATCAAACGAACTGTGGCTGCACCA16520.10813938721886704No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15900.10408088721428486No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG15880.10394996785929836No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15410.10087336301711508No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT851.20944605E-860.28179670
TTGTACG502.5340487E-643.98504365
GGTATCA12600.041.6378971
GTCGTTA350.001216004640.2633552
CGCCCTA1901.1641532E-1038.5259670
GTATCAA34150.038.3572851
GTACGCG501.1084184E-437.67961567
GTACGTA855.1010284E-737.47002869
TATCAAC37800.034.4563262
ATCAACG40050.032.4350433
TCAACGC40800.031.749664
AACGCAG41450.031.49766
CAACGCA41400.031.4549715
GTTCGCG802.9375879E-629.95027437
ACGCAGA43800.029.8848827
CGCAGAG43850.029.6194068
AGAGTAC44550.029.38176711
GGTACGT953.5442827E-629.2365868
GCAGAGT45650.028.5255899
TACGACC606.0905993E-428.1850764
Produced by FastQC (version 0.11.5)