FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692565

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692565
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences523978
Sequences flagged as poor quality0
Sequence length20-76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT8140.15535003378004422No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7840.14962460255964943No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC7530.14370832363190822No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT6280.11985236021359676No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCAC5800.11069167026096516No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT5420.10343945738179848No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGC1650.042.021563
GGTATCA17000.039.8145451
ACTAGCG2850.039.60787627
CCTAACG1800.038.5197642
TCTATCG350.001561155338.2631531
CTCGTAT1350.038.22879839
CTAGCGC2950.037.20405628
TAGCGCT2950.036.1775229
GTATCAA40100.035.898721
GACTAGC3200.035.19470226
TATACAC3200.035.08053
CGAGACT3150.034.61560423
TCGTATG1600.034.58823440
CGCTCAT2550.034.23613432
ACGAGAC3200.034.0682322
GCGCTCA2850.034.0676331
ACGGGTA1750.033.96352416
CCCACGA3150.033.55062519
CGATTAA1201.6667616E-533.41235470
CGTAGTG450.00346818132.51691454
Produced by FastQC (version 0.11.5)