FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004692567

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692567
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1477103
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC48380.32753301563939685No Hit
GACCTAGGACGGTCACCCTGGTCCCTCCGCCGAACACCCAGCCAACACCAGAGAGGAGCAGGCAAAAATACTCAG42900.29043336855994467No Hit
GCCTTGGGCTGACCTAGGACGGTCACCCTGGTCCCTCCGCCGAACACCCA42840.29002716804447626No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT38000.2572603264633543No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG37110.25123501881723886No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG34360.2326174951916014No Hit
GTGGTAACAGTCCACACTGGTTCCGGCAGAAACCTGGACAATCACCCAGG33550.22713378823277725No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC28980.19619484897126335No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA28600.1936222457066298No Hit
GGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCA27360.18522743505361508No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCCTGGTCCCTC27070.18326413256218424No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC26830.18163933050031042No Hit
GTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAGGGACAGTCAC25830.1748693219091695No Hit
GATTTATGGTACAAGCAACAAATACTCCTGGACGCCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAGGCTGC23560.15950140240727967No Hit
TATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCT23210.15713189940038036No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG22930.15523629699486088No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG22390.1515804923556448No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT22040.14921098934874547No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC22020.14907558917692268No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC21960.1486693886614542No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG21450.14521668427997234No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21090.1427794811871616No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA20480.1386497759465657No Hit
ACCTAGGACGGTCACCCTGGTCCCTCCGCCGAACACCCAGCCAACACCAGAGAGGAGCAGGCAAAAATACTCAGC20410.1381758753451858No Hit
CTCACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGCTTCCAGCACTGGAGCAGTCACCAGTGGTAA19910.13479087104961535No Hit
GCCTGAGGACGAGGCTGAGTATTTTTGCCTGCTCCTCTCTGGTGTTGGCT19750.1337076696750328No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC19750.1337076696750328No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19710.13343686933138718No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG18810.12734386159936037No Hit
GTCCACACTGGTTCCGGCAGAAACCTGGACAATCACCCAGGGCACTGATTTATGGTACAAGCAACAAATACTC18800.12727616151344898No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC18530.12544825919384092No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA18530.12544825919384092No Hit
GTTCTCAGGCTCCCTCCTTGGGGGCAAGGCTGCCCTGACACTGTCAGGTG18360.12429735773334698No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT17910.12125085386733354No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC17820.12064155309413087No Hit
ATATAAAGGGGGATGGAAGTGAGAAGTACTATTTGGACTCTATGAAGGGCCGATTCACCGTCTCCAGAGACAACG17360.11752734914220607No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC16810.11380384441707857No Hit
GTAATACACGGCCGTGTCTTCGGCTCTCAGGCTGCTTATGTGAAGATACA16600.11238214261293897No Hit
CACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGCTTCCAGC16470.11150204149609065No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT16170.10947103891874839No Hit
GCCCTGACACTGTCAGGTGTGCAGCCTGAGGACGAGGCTGAGTATTTTTG15640.1058829343654437No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA15110.10229482981213903No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT15050.10188862929667057No Hit
GTGTCAGGGCAGCCTTGCCCCCAAGGAGGGAGCCTGAGAACCGGGCAGGCGTCCAGGAGTATTTGTTGCTTGTAC14960.10127932852346788No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA14940.10114392835164508No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA14860.10060232766435381No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)