FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692568

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692568
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1477103
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC44600.30194238316488425No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG40950.27723185180721993No Hit
GTGGTAACAGTCCACACTGGTTCCGGCAGAAACCTGGACAATCACCCAGG40130.27168044476248443No Hit
GACCTAGGACGGTCACCCTGGTCCCTCCGCCGAACACCCAGCCAACACCAGAGAGGAGCAGGCAAAAATACTCAG39750.26910784149785083No Hit
GCCTTGGGCTGACCTAGGACGGTCACCCTGGTCCCTCCGCCGAACACCCA38230.2588174284393167No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT36150.2447358105697436No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG32540.22029607955572497No Hit
GGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCA31080.21041186701265926No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC29680.20093385498506197No Hit
GTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAGGGACAGTCAC28530.19314834510524992No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26670.18055612912572785No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG26380.178592826634297No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCCTGGTCCCTC25500.172635219074093No Hit
GATTTATGGTACAAGCAACAAATACTCCTGGACGCCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAGGCTGC25150.17026571606719368No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA24410.1652559097097494No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG23980.16234480601555884No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC23530.15929830214954543No Hit
TATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCT23040.15598099793988637No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG22690.15361149493298706No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT22650.15334069458934144No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC22080.14948178969239112No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG21770.14738308702913744No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA20790.14074847860981934No Hit
GTCCACACTGGTTCCGGCAGAAACCTGGACAATCACCCAGGGCACTGATTTATGGTACAAGCAACAAATACTC20070.1358740724241979No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG19510.132082867613159No Hit
GCCTGAGGACGAGGCTGAGTATTTTTGCCTGCTCCTCTCTGGTGTTGGCT19290.130593465723108No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG19200.12998416494990533No Hit
GTTCTCAGGCTCCCTCCTTGGGGGCAAGGCTGCCCTGACACTGTCAGGTG18620.1260575599670436No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC18600.12592215979522078No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC18170.12301105610103019No Hit
CTCACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGCTTCCAGCACTGGAGCAGTCACCAGTGGTAA17880.12104775360959932No Hit
ACCTAGGACGGTCACCCTGGTCCCTCCGCCGAACACCCAGCCAACACCAGAGAGGAGCAGGCAAAAATACTCAGC17840.1207769532659537No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC17740.1200999524068396No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA16680.11292374330023026No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT16570.11217904235520475No Hit
ATATAAAGGGGGATGGAAGTGAGAAGTACTATTTGGACTCTATGAAGGGCCGATTCACCGTCTCCAGAGACAACG16340.11062194037924233No Hit
GTAATACACGGCCGTGTCTTCGGCTCTCAGGCTGCTTATGTGAAGATACA16270.11014803977786249No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA16160.10940333883283698No Hit
CACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGCTTCCAGC15560.10534133367815243No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC15560.10534133367815243No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT15290.10351343135854439No Hit
GTGTCAGGGCAGCCTTGCCCCCAAGGAGGGAGCCTGAGAACCGGGCAGGCGTCCAGGAGTATTTGTTGCTTGTAC15180.1027687304135189No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC15070.1020240294684934No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC15060.10195632938258198No Hit
GTCCAATTCTCAGACTGTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCAC14870.1006700277502652No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)