FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692569

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692569
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1643971
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCATTATGGTAGCTCACGGACGTTCGGCCGAGGGACCAAGGTGGAAATCAACCGAACTGTGG69900.42518998206172737No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT53350.3245191064805888No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA47350.28802211231220015No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC45290.27549147764772003No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC36780.2237265742522222No Hit
GTGTATTACTGTCAGCATTATGGTAGCTCACGGACGTTCGGCCGAGGGAC34600.21046599970437435No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG34110.20748541184728928No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC32820.19963855810108572No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA32050.19495477718280918No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG31030.1887502881741831No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT29070.17682793674584285No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT28410.1728132673873201No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26120.15888358127971844No Hit
CTCTTGCAGGGCCAGTCAGAGTGTTAGCAGCAGCTACTTAGCCTGGTACC25430.15468642695035376No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA25240.1535306888016881No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCTTGCAGGGCCAGTCAGAGTGTTAGCAG25210.15334820383084619No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT25010.15213163735856655No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG24630.14982016106123525No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA23270.14154750904973384No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22940.13954017437047248No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22500.1368637281314573No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT22380.13613378824808955No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20510.12475889173227507No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20170.12269072872939973No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20050.12196078884603195No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19810.1205009090792964No Hit
GCATTATGGTAGCTCACGGACGTTCGGCCGAGGGACCAAGGTGGAAATCAACCGAACTGTGGCTGCACCAT19800.12044008075568244No Hit
TCTCTGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT19750.12013593913761253No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC19330.11758114954582531No Hit
GTGCAGCCACAGTTCGGTTGATTTCCACCTTGGTCCCTCGGCCGAACGTC19050.11587795648463385No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCTTGC18770.11417476342344239No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGGTTGATTTCCACCTTGGTCC18720.11387062180537248No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT18380.11180245880249712No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT18010.10955181082877982No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC17980.10936932585793788No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCTTGCAGGGCCAGTCAGA17930.10906518423986798No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCATTATGGTAGCTCA17880.10876104262179807No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17700.10766613279674642No Hit
GTAATACAAGGCGGTGTCCTCAGCTCTCAGACTGTTCATTTGCAAATACA17540.10669287961892271No Hit
GTCAGAGTGTTAGCAGCAGCTACTTAGCCTGGTACCAGCAGAAACCTGGC17370.10565879811748505No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17060.10377312008545163No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17050.10371229176183763No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16440.1000017640213848No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATGCG200.006670333453.05294454
CGATTGT753.275417E-546.32892670
TACGTAT1003.6577349E-641.69603370
GGTATCA14150.040.9738351
GTATCAA35250.037.2236331
TACTCGA608.621959E-636.8041266
TGGACCG400.002828598333.9031875
CCTAACG400.002828598333.9031872
TATCAAC40300.032.2206462
ATCAACG41050.031.714553
CGTATGC1207.6397555E-1131.64591241
TCAACGC41250.031.5607814
CAACGCA41600.031.213755
AACGCAG41950.031.1140166
ACGAGAC2300.030.9541422
TCAATCG802.341223E-630.83501452
ACTCGAT753.5716257E-529.90047667
GTCTATA803.1635136E-629.6670931
TCGATTG754.5694795E-429.60303769
ACGCAGA44450.029.3596067
Produced by FastQC (version 0.11.5)