FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692571

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692571
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1103354
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACGGCCGTGTCGTCGACTCTCAGGCTGTTCATTTGCAGAGACA30180.273529619686882No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT28820.26120356657971966No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC27580.2499651063937775No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATCA25480.23093223027242388No Hit
CTATAATAACTGGCCTCCCACTTTCGGCGGAGGGACCAAGTTGGAGATCAAACGAACTGTGGCTGCACCAT23700.21479960194099082No Hit
GTATATTACTGTGCGAAAGGGCTGTATTATTATGATAGTACTGGTTATAG22660.20537379662374905No Hit
GTTATTATAGTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGCGTGAACT21370.19368217272063182No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT19870.18008726120537924No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19510.1768244824417186No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT19030.1724741107568378No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA17910.16232324349211585No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG17270.15652274791227475No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17030.15434756206983435No Hit
GTCTGTGTCTGCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTGGCAACAACTTAGCCTGGTA16340.14809390277281814No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15890.14401542931824238No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCATTGGGTCTGGGACAGAGTTCACG15550.1409339160414518No Hit
GTCTCCAGCCACCCTGTCTGTGTCTGCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTGGCAA15440.13993695586366658No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15340.13903062842931643No Hit
GTCCCAGGCTCCTCATCCATGGTGCATCCACCAGGGCCACTGGTATCCCA15160.1373992390474861No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14840.13449899125756556No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14740.1335926638232154No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT14730.1335020310797804No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14730.1335020310797804No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC14340.1299673540858147No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTGGCAACAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGGTCCCAGGCT14330.12987672134237968No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC14100.1277921682433743No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG14050.1273390045261992No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14030.12715773903932917No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC13780.12489192045345374No Hit
GTCTGGGACAGAGTTCACGCTCACCATCAGCAGCCTGCAGTCTGAAGATT13600.12326053107162344No Hit
GTCCCAGACCCAATGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGGT13560.12289800009788338No Hit
GCCTGGTACAGCCTGGGGGGTCCCGGAGACTCCTCTGTGCAGCCTCTGGA13540.12271673461101333No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC13500.12235420363727326No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13100.11872889389987257No Hit
GTGTTAGTGGTGGTAGCACATACTACGCAGACTCCGTGAAGGGCCGGTTC13010.1179131992089574No Hit
GTTTATTACTGTCAGCACTATAATAACTGGCCTCCCACTTTCGGCGGAGGGACCAAGTTGGAGATCAAACGAA12930.11718813726147728No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG12800.11600991159682206No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12700.11510358416247188No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG12570.11392535849781667No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT12430.11265650008972641No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG12410.11247523460285638No Hit
GTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGCGTGAACTCTGTCCCAGACCCAATGCCA12400.11238460185942135No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAACTTGGTCCCTCCGCCGAAAGTG12370.11211270362911632No Hit
TAATAATACAGCCCTTTCGCACAGTAATATACGGCCGTGTCGTCGACTCT12160.11020941601698096No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT12110.10975625229980586No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12040.10912182309576074No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12030.10903119035232574No Hit
GTCAGAGTGTTGGCAACAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGGTCCCAGGCTCCTCATCCATGGTG12010.1088499248654557No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG11840.1073091682270604No Hit
ATAATAACTGGCCTCCCACTTTCGGCGGAGGGACCAAGTTGGAGATCAAA11610.105224615128055No Hit
GAGTTCACGCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCACTATAATAACTGG11590.10504334964118497No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC11460.10386512397652975No Hit
CCTCTGTGCAGCCTCTGGATATATTTTTAACAACTATGCCATGAACTGGGTCCGCCAGGCCCCAGGGAAGGGG11140.10096487618660918No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTTAG150.00255296567.613331
GCAAGTT200.006491649453.41717556
TCTGGAG11350.046.68020270
GGTATCA8600.044.0272831
TCGTATG4300.042.41860240
CGTATGC4550.041.6891541
ATGCCGT4500.041.6575944
ATCTCGT3900.040.36490637
GCCGTCT4700.040.04897746
CATGCGC5000.039.9806728
CCGTCTT4900.039.92291647
GTATCAA24650.039.7725451
CTTATAC5050.039.49691
ACGAGAC5100.039.11853822
ATATCGT350.001402381539.1113437
ACATGCG5150.038.78975327
ATGCGCA5250.038.09759529
TATACAC5350.037.9105763
GTATGCC5200.037.8833242
CGTCTTC4900.037.8711148
Produced by FastQC (version 0.11.5)