FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692572

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692572
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1103354
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG45480.41219771714245834No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT31530.2857650400506093No Hit
GTAATATACGGCCGTGTCGTCGACTCTCAGGCTGTTCATTTGCAGAGACA29350.2660071019817756No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATCA25280.22911957540372357No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24650.22340971256731745No Hit
CTATAATAACTGGCCTCCCACTTTCGGCGGAGGGACCAAGTTGGAGATCAAACGAACTGTGGCTGCACCAT22390.20292671255100359No Hit
GTTATTATAGTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGCGTGAACT21050.19078192493071128No Hit
GTATATTACTGTGCGAAAGGGCTGTATTATTATGATAGTACTGGTTATAG20890.189331801035751No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT19890.18026852669224927No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17980.162957672696161No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17750.16087311959715558No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17200.15588831870822964No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17080.15480072578700943No Hit
GTCTGTGTCTGCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTGGCAACAACTTAGCCTGGTA16270.147459473568773No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15890.14401542931824238No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC15630.14165897798893193No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15530.14075265055458175No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG15500.1404807523242767No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCATTGGGTCTGGGACAGAGTTCACG15310.13875873019901136No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA14920.1352240532050457No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTGGCAACAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGGTCCCAGGCT14750.1336832965666504No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT14420.13069241603329484No Hit
GCCTGGTACAGCCTGGGGGGTCCCGGAGACTCCTCTGTGCAGCCTCTGGA14140.12815469921711437No Hit
GTCCCAGGCTCCTCATCCATGGTGCATCCACCAGGGCCACTGGTATCCCA13860.12561698240093389No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13860.12561698240093389No Hit
GTCTCCAGCCACCCTGTCTGTGTCTGCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTGGCAA13710.12425749124940862No Hit
GTGTTAGTGGTGGTAGCACATACTACGCAGACTCCGTGAAGGGCCGGTTC13560.12289800009788338No Hit
GTCCCAGACCCAATGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGGT13560.12289800009788338No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAACTTGGTCCCTCCGCCGAAAGTG13530.12262610186757832No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC13420.12162914168979314No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12960.11746003549178233No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12900.11691623903117222No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC12830.1162818098271271No Hit
GTCTGGGACAGAGTTCACGCTCACCATCAGCAGCCTGCAGTCTGAAGATT12830.1162818098271271No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12790.11591927885338704No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG12460.11292839832003146No Hit
GTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGCGTGAACTCTGTCCCAGACCCAATGCCA12320.11165953991194122No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA12250.1110251107078961No Hit
GTTTATTACTGTCAGCACTATAATAACTGGCCTCCCACTTTCGGCGGAGGGACCAAGTTGGAGATCAAACGAA12180.11039068150385098No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG12150.11011878327354593No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12060.10930308858263077No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12020.10894055760889071No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11690.10594967707553515No Hit
GAGTTCACGCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCACTATAATAACTGG11680.10585904433210014No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11360.10295879654217957No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11270.10214310185126443No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC11160.10114614167347923No Hit
GTATTATTATGATAGTACTGGTTATAGAGTCTTTGACTACTGGGGCCAGG11160.10114614167347923No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT11080.10042107972599909No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT11070.10033044698256406No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)