FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692575

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692575
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1147981
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC87410.7614237517868327No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT51530.44887502493508163No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA48650.4237875017095231No Hit
CCCCTGAGGGGCGAATAGTGAAATCAAAAATGATGAGTTTGGGGGCTTTGCCTGGGCGTTGTTGGTACCAGGAGA48340.42108710858454973No Hit
GTCCTGGGCCCAGTCTGACCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG46740.4071495956814616No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC42570.3708249526777882No Hit
GACCTAAGACAGTGACCTTGGTCCCAGGTCCGAAGACATAAAGGGGAGTG42540.3705636243108553No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG41580.36220111656900245No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC37730.32866397614594667No Hit
ACCTAAGACAGTGACCTTGGTCCCAGGTCCGAAGACATAAAGGGGAGTGG35770.3115905228396637No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC35700.3109807566501536No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCATTGGAACCAGCACTGACCCT34240.2982627761260857No Hit
CCTCAGGGGTGTCTAGTCGCTTCTCTGGGTCCGAGTCTGACAACACGGCCTCCCTGACCATCTCTGGGCTCCAGC33000.28746120362619243No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC30850.26873267066266776No Hit
TCTCTGGGCTCCAGCCTGAGGACGAGGCTGACTATTACTGCAGCTCATAT30710.26751313828364753No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC30470.26542251134818434No Hit
GACTATTACTGCAGCTCATATAAAGCCGGGGCCACTCCCCTTTATGTCTTCGGACCTGGGACCAAGGTCA28630.249394371509633No Hit
GCCTTGGGCTGACCTAAGACAGTGACCTTGGTCCCAGGTCCGAAGACATA28260.24617132165079386No Hit
GTCGATCACCATCTCCTGCATTGGAACCAGCACTGACCCTGGTACTTACAAGTATGTCTCCTGGTACCAACAACG27590.24033498812262571No Hit
CCGCTATTCCTCACCCTCCTCACTCAGGTCACAGGGTCCTGGGCCCAGTC27150.2365021720742765No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAAGACAGTGACCTTGGTC26830.23371466949365888No Hit
CCCTGGTACTTACAAGTATGTCTCCTGGTACCAACAACGCCCAGGCAAAGCCCCCAAACTCATCATTTTTGATTT25720.22404551991714147No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT25700.2238713010058529No Hit
GTAATAGTCAGCCTCGTCCTCAGGCTGGAGCCCAGAGATGGTCAGGGAGG25650.2234357537276314No Hit
GGTCACAGGGTCCTGGGCCCAGTCTGACCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC25610.22308731590505418No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG24770.2157701216309329No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT24470.2131568379616039No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT24190.21071777320356347No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG23510.20479433021975102No Hit
CTATTACTGCAGCTCATATAAAGCCGGGGCCACTCCCCTTTATGTCTTCG23440.20418456403024093No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG21960.1912923645948844No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC21220.18484626487720615No Hit
GCAGTAATAGTCAGCCTCGTCCTCAGGCTGGAGCCCAGAGATGGTCAGGG21170.18441071759898467No Hit
GCTTTATATGAGCTGCAGTAATAGTCAGCCTCGTCCTCAGGCTGGAGCCC20220.17613531931277607No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG19870.17308648836522555No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCATTGGAACCAGCACTGACC19770.17221539380878254No Hit
GGCCTGGGCTCCGCTATTCCTCACCCTCCTCACTCAGGTCACAGGGTCCTGGGCCCAGTCTGACCTGACTCAGC19520.17003765741767504No Hit
GCACTGACCCTGGTACTTACAAGTATGTCTCCTGGTACCAACAACGCCCA19440.16934078177252063No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA18820.163939995522574No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA18330.15967163219600325No Hit
CCTTTATGTCTTCGGACCTGGGACCAAGGTCACTGTCTTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTT17540.1527899852001035No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC17440.15191889064366046No Hit
GTTTGGGGGCTTTGCCTGGGCGTTGTTGGTACCAGGAGACATACTTGTAA17260.15035092044206308No Hit
GTATGTCTCCTGGTACCAACAACGCCCAGGCAAAGCCCCCAAACTCATCA15990.13928801957523687No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC15950.13893958175265966No Hit
GACCTAAGACAGTGACCTTGGTCCCAGGTCCGAAGACATAAAGGGGAGTGGCCCCGGCTTTATATGAGCTGCAGT15570.13562942243817624No Hit
AACCAGCACTGACCCTGGTACTTACAAGTATGTCTCCTGGTACCAACAACGCCCAGGCAAAGCCCCCAAACTCAT15520.13519387515995474No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT15430.13440989005915602No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGTCAGACTCGGACCCAGAGAAG15360.13380012386964593No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG15330.13353879550271303No Hit
ACCATGGCCTGGGCTCCGCTATTCCTCACCCTCCTCACTCAGGTCACAGGGTCCTGGGCCCAGTCTGACCTGACT15190.1323192631236928No Hit
AGTCTGACCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCG14940.13014152673258528No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC14930.130054417276941No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT14820.12909621326485368No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCCGCTATTCCTCACCCTCCTCACTCAGGTCACAGGGTCCTGGGC14730.128312228164055No Hit
GAGATGGTCAGGGAGGCCGTGTTGTCAGACTCGGACCCAGAGAAGCGACT14600.12717980524067907No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA14410.12552472558343736No Hit
CTCCTGCATTGGAACCAGCACTGACCCTGGTACTTACAAGTATGTCTCCTGGTACCAACAACGCCCAGGCAAAGC14370.12517628776086015No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT14330.12482784993828296No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT14270.12430519320441714No Hit
GGCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGTCAGACTCGGACCC14090.12273722300281972No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG14080.12265011354717544No Hit
ATATAAAGCCGGGGCCACTCCCCTTTATGTCTTCGGACCTGGGACCAAGGTCACTGTCTTAGGTCAGCCCAAGGC14040.12230167572459823No Hit
GACCATCTCTGGGCTCCAGCCTGAGGACGAGGCTGACTATTACTGCAGCT13840.12055948661171222No Hit
ATTCCTCACCCTCCTCACTCAGGTCACAGGGTCCTGGGCCCAGTCTGACC13680.1191657353214034No Hit
AGACACCCCTGAGGGGCGAATAGTGAAATCAAAAATGATGAGTTTGGGGG13480.1174235462085174No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC13340.11620401382949719No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG13300.11585557600691998No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC13260.11550713818434277No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC13080.11393916798274537No Hit
GGCAAAGCCCCCAAACTCATCATTTTTGATTTCACTATTCGCCCCTCAGG13080.11393916798274537No Hit
GTCCAGGAGACCCAGACACGGAGGCAGGCTGAGTCAGGTCAGACTGGGCC13020.11341651124887955No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG13010.11332940179323527No Hit
GTCTAGTCGCTTCTCTGGGTCCGAGTCTGACAACACGGCCTCCCTGACCA13010.11332940179323527No Hit
CGTCTCCACCATGGCCTGGGCTCCGCTATTCCTCACCCTCCTCACTCAGGTCACAGGGTCCTGGGCCCAGTCTGA12990.11315518288194666No Hit
GGTACCAACAACGCCCAGGCAAAGCCCCCAAACTCATCATTTTTGATTTC12980.11306807342630235No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG12840.11184854104728213No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC12830.11176143159163784No Hit
TTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCA12770.11123877485777203No Hit
GTACCAGGGTCAGTGCTGGTTCCAATGCAGGAGATGGTGATCGACTGTCCAGGAGACCCAGACACGGAGGCAGG12660.11028057084568474No Hit
GCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCATTGGAACCAGCACTGACCCTGGTA12660.11028057084568474No Hit
TCCTCACTCAGGTCACAGGGTCCTGGGCCCAGTCTGACCTGACTCAGCCT12390.1079286155432886No Hit
GTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTT12290.1070575209868456No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA12160.1059250980634697No Hit
CTCGGGACGTCTCCACCATGGCCTGGGCTCCGCTATTCCTCACCCTCCTC12050.10496689405138239No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG11910.10374736167236218No Hit
TCCTCACCCTCCTCACTCAGGTCACAGGGTCCTGGGCCCAGTCTGACCTGACTCAGCCTGCCTCCGTGTCTGGGT11850.10322470493849636No Hit
GCCTGAGGACGAGGCTGACTATTACTGCAGCTCATATAAAGCCGGGGCCA11770.10252782929334196No Hit
GCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGA11520.10035009290223443No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)