FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692576

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692576
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1147981
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC78820.6865967293883783No Hit
GTCCTGGGCCCAGTCTGACCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG50000.4355472782215037No Hit
CCCCTGAGGGGCGAATAGTGAAATCAAAAATGATGAGTTTGGGGGCTTTGCCTGGGCGTTGTTGGTACCAGGAGA47680.41533788451202586No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT47370.4126374913870526No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC45580.3970448988267227No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA45410.3955640380807696No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC38730.33737492171037675No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG37630.32779288158950365No Hit
GACCTAAGACAGTGACCTTGGTCCCAGGTCCGAAGACATAAAGGGGAGTG36860.3210854535048925No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC34260.2984369950373743No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC32920.286764327981038No Hit
CCTCAGGGGTGTCTAGTCGCTTCTCTGGGTCCGAGTCTGACAACACGGCCTCCCTGACCATCTCTGGGCTCCAGC31360.27317525290052713No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCATTGGAACCAGCACTGACCCT31220.2719557205215069No Hit
GACTATTACTGCAGCTCATATAAAGCCGGGGCCACTCCCCTTTATGTCTTCGGACCTGGGACCAAGGTCA29270.2549693766708683No Hit
ACCTAAGACAGTGACCTTGGTCCCAGGTCCGAAGACATAAAGGGGAGTGG29070.25322718755798224No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC28690.2499170282434988No Hit
GTCGATCACCATCTCCTGCATTGGAACCAGCACTGACCCTGGTACTTACAAGTATGTCTCCTGGTACCAACAACG28240.24599710273950529No Hit
CCGCTATTCCTCACCCTCCTCACTCAGGTCACAGGGTCCTGGGCCCAGTC28000.24390647580404207No Hit
TCTCTGGGCTCCAGCCTGAGGACGAGGCTGACTATTACTGCAGCTCATAT27920.24320960015888765No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG27530.23981233138875993No Hit
GCCTTGGGCTGACCTAAGACAGTGACCTTGGTCCCAGGTCCGAAGACATA26980.23502131132832338No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT26170.22796544542113503No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAAGACAGTGACCTTGGTC26150.2277912265098464No Hit
GGTCACAGGGTCCTGGGCCCAGTCTGACCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC26090.22726856977598062No Hit
CCCTGGTACTTACAAGTATGTCTCCTGGTACCAACAACGCCCAGGCAAAGCCCCCAAACTCATCATTTTTGATTT24900.21690254455430882No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT23890.20810448953423444No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG23730.20671073824392563No Hit
GTAATAGTCAGCCTCGTCCTCAGGCTGGAGCCCAGAGATGGTCAGGGAGG23430.20409745457459663No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG23370.2035747978407308No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT22910.19956776288109299No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22530.19625760356660957No Hit
CTATTACTGCAGCTCATATAAAGCCGGGGCCACTCCCCTTTATGTCTTCG22250.1938185388085691No Hit
GCACTGACCCTGGTACTTACAAGTATGTCTCCTGGTACCAACAACGCCCA20700.18031657318370253No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG20570.1791841502603266No Hit
GGCCTGGGCTCCGCTATTCCTCACCCTCCTCACTCAGGTCACAGGGTCCTGGGCCCAGTCTGACCTGACTCAGC20520.1787486029821051No Hit
GCTTTATATGAGCTGCAGTAATAGTCAGCCTCGTCCTCAGGCTGGAGCCC20220.17613531931277607No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA19410.16907945340558772No Hit
GCAGTAATAGTCAGCCTCGTCCTCAGGCTGGAGCCCAGAGATGGTCAGGG18940.16498530899030558No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC18830.16402710497821826No Hit
GTTTGGGGGCTTTGCCTGGGCGTTGTTGGTACCAGGAGACATACTTGTAA18810.1638528860669297No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCATTGGAACCAGCACTGACC17400.15157045282108328No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA17240.15017670153077448No Hit
GACCTAAGACAGTGACCTTGGTCCCAGGTCCGAAGACATAAAGGGGAGTGGCCCCGGCTTTATATGAGCTGCAGT17140.14930560697433146No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC16620.14477591528082784No Hit
GTATGTCTCCTGGTACCAACAACGCCCAGGCAAAGCCCCCAAACTCATCA16520.1439048207243848No Hit
CCTTTATGTCTTCGGACCTGGGACCAAGGTCACTGTCTTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTT16330.14224974106714308No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC16320.1421626316114988No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC16020.13954934794216978No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT16010.13946223848652547No Hit
AACCAGCACTGACCCTGGTACTTACAAGTATGTCTCCTGGTACCAACAACGCCCAGGCAAAGCCCCCAAACTCAT15390.13406145223657884No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT15290.13319035768013582No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT15000.1306641834664511No Hit
ACCATGGCCTGGGCTCCGCTATTCCTCACCCTCCTCACTCAGGTCACAGGGTCCTGGGCCCAGTCTGACCTGACT14960.13031574564387388No Hit
GGCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGTCAGACTCGGACCC14900.1297930889100081No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGTCAGACTCGGACCCAGAGAAG14900.1297930889100081No Hit
CTCCTGCATTGGAACCAGCACTGACCCTGGTACTTACAAGTATGTCTCCTGGTACCAACAACGCCCAGGCAAAGC14900.1297930889100081No Hit
ATATAAAGCCGGGGCCACTCCCCTTTATGTCTTCGGACCTGGGACCAAGGTCACTGTCTTAGGTCAGCCCAAGGC14520.12648292959552468No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCCGCTATTCCTCACCCTCCTCACTCAGGTCACAGGGTCCTGGGC14490.12622160122859175No Hit
GGGGTGTCTAGTCGCTTCTCTGGGTCCGAGTCTGACAACACGGCCTCCCT14340.12491495939392724No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG14070.12256300409153115No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG14050.12238878518024254No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT13900.12108214334557801No Hit
GGCAAAGCCCCCAAACTCATCATTTTTGATTTCACTATTCGCCCCTCAGG13850.1206465960673565No Hit
GAGATGGTCAGGGAGGCCGTGTTGTCAGACTCGGACCCAGAGAAGCGACT13460.1172493272972288No Hit
AGTCTGACCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCG13450.11716221784158448No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC13410.11681378001900729No Hit
GACCATCTCTGGGCTCCAGCCTGAGGACGAGGCTGACTATTACTGCAGCT13400.11672667056336297No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG13380.11655245165207437No Hit
ATTCCTCACCCTCCTCACTCAGGTCACAGGGTCCTGGGCCCAGTCTGACC13370.11646534219643008No Hit
GCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCATTGGAACCAGCACTGACCCTGGTA13330.11611690437385287No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC13240.11533291927305417No Hit
AGACACCCCTGAGGGGCGAATAGTGAAATCAAAAATGATGAGTTTGGGGG13230.11524580981740987No Hit
CGTCTCCACCATGGCCTGGGCTCCGCTATTCCTCACCCTCCTCACTCAGGTCACAGGGTCCTGGGCCCAGTCTGA13100.11411338689403396No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC13070.11385205852710105No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG12940.11271963560372515No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC12930.11263252614808084No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA12890.11228408832550366No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA12670.11036768030132903No Hit
TTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCA12620.10993213302310752No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC12610.10984502356746323No Hit
GTCTAGTCGCTTCTCTGGGTCCGAGTCTGACAACACGGCCTCCCTGACCA12540.10923525737795313No Hit
CTCGGGACGTCTCCACCATGGCCTGGGCTCCGCTATTCCTCACCCTCCTC12110.10548955078524817No Hit
GTCCAGGAGACCCAGACACGGAGGCAGGCTGAGTCAGGTCAGACTGGGCC12090.1053153318739596No Hit
GGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGC12020.10470556568444948No Hit
GTACCAGGGTCAGTGCTGGTTCCAATGCAGGAGATGGTGATCGACTGTCCAGGAGACCCAGACACGGAGGCAGG11940.10400869003929507No Hit
GTGCTGGTTCCAATGCAGGAGATGGTGATCGACTGTCCAGGAGACCCAGA11920.10383447112800646No Hit
TCCTCACCCTCCTCACTCAGGTCACAGGGTCCTGGGCCCAGTCTGACCTGACTCAGCCTGCCTCCGTGTCTGGGT11880.10348603330542927No Hit
GTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTT11560.10069853072481165No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG11550.10061142126916735No Hit
GAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG11500.10017587399094585No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9250.044.0774271
ACGATCA503.0505726E-642.8134759
GTATCAA23000.037.078541
GTGGTAT2800.036.4032331
CGTGTCA400.002512403834.73188446
GAATACG1350.032.703719
GACGATC552.7065052E-432.36924458
GGTACTA450.003870688131.79956460
TATCAAC26700.031.7978152
AATACGT1400.031.5357210
ATACGTA1400.031.53434811
TGGTATC3350.031.4257322
AACAAGG40100.031.18074470
CGTACCA1450.030.43899514
ATCAACG28200.030.344653
TCAACGC28550.029.9700374
CAACGCA29050.029.4593335
ACGTACC1501.8189894E-1229.42436413
AACGCAG29300.029.2092466
TACAATC755.2496685E-528.2577355
Produced by FastQC (version 0.11.5)