FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692577

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692577
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1015447
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGAGA39250.38652928217819343No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT38420.3783555419435973No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA35990.3544251940278518No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG28630.28194479869456507No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC28440.2800737015324286No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC27880.27455888884402635No Hit
GATTATTACTGCAGCTCATATACAAGCAGCAGAACTCTTTATGTCTTCGG27010.2659912334174014No Hit
GTCCCAACACCACCCAGGCAAAGCCCCCAAACTCATAATTTATGATGTCA26880.264711009043308No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC25860.25466617164657535No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC25650.25259811688842454No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACTAGCAGTGACGTTGGTGGTTATTACTATGTCTCCTGGT24320.23950043675346916No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC24240.23871260636941172No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG24230.2386141275714045No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAAGAGTTCTGC24060.2369399880052824No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAAGAGTTCTG23920.23556128483318184No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC23920.23556128483318184No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC22180.218425973979932No Hit
GTGTATTACTGTGCGACAACCTATTATTATGATAGTAGTGGTTATTCACCGTTCGACCCCTGGGGCCAGGGAACC21940.2160624828277596No Hit
GTGTTGGGACCAGGAGACATAGTAATAACCACCAACGTCACTGCTAGTTCCAGTGCAGGAGATGGTGATCGA21670.21340355528156563No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAAGAGTTCTGCTGCTTGTATATGAG20780.20463894225892634No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATAT20770.20454046346091917No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG18330.18051163674716653No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG18100.1782466243930013No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT18070.17795118799897974No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAAGAGTT17940.17667096362488638No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT17880.17608009083684328No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG17810.17539073925079302No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT17780.17509530285677147No Hit
GTCTCTGGTGGCTCCATCAGCAGTAGTAGTTACTACTGGGGCTGGATCCG17340.1707622357444554No Hit
GTCGATCACCATCTCCTGCACTGGAACTAGCAGTGACGTTGGTGGTTATTACTATGTCTCCTGGTCCCAACACCA17000.16741395661221117No Hit
CTCCTGCACTGGAACTAGCAGTGACGTTGGTGGTTATTACTATGTCTCCTGGTCCCAACACCACCCAGGCAAAGC16350.1610128347417443No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG15700.15461171287127737No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC15570.153331488497184No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC15560.1532330096991768No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG14930.14702884542472427No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT14890.14663493023269555No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA13840.13629465644194133No Hit
GGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC13680.13471899567382642No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA13250.13048440735951755No Hit
CTTTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTC13170.12969657697546008No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG12990.12792395861133077No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC12420.12231066712492134No Hit
CAGTAATACACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGA12190.12004565477075613No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTAGTAGTTACTACTGGG12100.1191593455886915No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA12010.11827303640662684No Hit
GACATAAAGAGTTCTGCTGCTTGTATATGAGCTGCAGTAATAATCAGCCT11910.11728824842655501No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC11480.11305366011224614No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT11360.11187191453615992No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTACCAGACTTAGAGCCAGAGAAG11280.11108408415210248No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC11150.1098038597780091No Hit
GGTTATTACTATGTCTCCTGGTCCCAACACCACCCAGGCAAAGCCCCCAAACTCATAATTTATGATGTCAGTAAT11080.10911450819195881No Hit
CTATTATAGTGGGAGCACCTACTACAACCCGTCCCTCAAGAGTCGAGTCACCATATCCGTAGACACGTCCATGAA11030.10862211420192289No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG10850.1068494958377936No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC10820.10655405944377207No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG10750.10586470785772177No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT10570.10409208949359247No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG10550.10389513189757811No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGAT11300.062.2368470
CGTATGC2100.055.8645641
TAGTACG252.6998576E-454.639134
GCGCGCA200.00695798252.48811753
ATGCCGT2400.050.47992344
TCGTATG2350.049.87188340
GTATGCC2500.046.9729742
GTCGTAT306.376848E-445.90332439
ACCTCTC2700.045.5707627
ATCTCGT1900.045.21403537
CACGAGA2650.045.10527421
ACGAGAC2650.045.10527422
CTCTCTA2800.043.9952729
CCCACGA2800.043.9042819
CGAGCCC2800.043.9042815
CCGTCTT2600.042.75221647
GCCGTCT2650.041.89939546
TACATCT2050.041.81865734
GTATCAA23150.041.1563641
CGTCTTC2550.040.92143248
Produced by FastQC (version 0.11.5)