FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692578

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692578
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1015447
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGAGA38880.3828855666519277No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT34030.33512334961844387No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC32780.32281349986754604No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA31460.3098142985305979No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC30480.3001633763258939No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG25630.25240115929241014No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG25600.25210572289838856No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC25290.24905288016016594No Hit
GATTATTACTGCAGCTCATATACAAGCAGCAGAACTCTTTATGTCTTCGG25290.24905288016016594No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACTAGCAGTGACGTTGGTGGTTATTACTATGTCTCCTGGT25240.24856048617013No Hit
GTCCCAACACCACCCAGGCAAAGCCCCCAAACTCATAATTTATGATGTCA25220.24836352857411564No Hit
GTGTATTACTGTGCGACAACCTATTATTATGATAGTAGTGGTTATTCACCGTTCGACCCCTGGGGCCAGGGAACC23200.22847081137666467No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC23110.2275845021946No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23040.22689515060854973No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC22690.22344839267829836No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC22680.22334991388029113No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC21800.21468377965565902No Hit
GTGTTGGGACCAGGAGACATAGTAATAACCACCAACGTCACTGCTAGTTCCAGTGCAGGAGATGGTGATCGA20950.20631308182504846No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAAGAGTTCTGC20770.20454046346091917No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATAT20680.20365415427885453No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC19840.19538193524625116No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG19540.19242757130603566No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAAGAGTTCTGCTGCTTGTATATGAG19040.18750363140567652No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAAGAGTTCTG18820.1853370978495185No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT18540.18257969150531736No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAAGAGTT18220.1794283699690875No Hit
GTCTCTGGTGGCTCCATCAGCAGTAGTAGTTACTACTGGGGCTGGATCCG17470.1720424601185488No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT17020.16761091420822552No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT16780.16524742305605314No Hit
CTCCTGCACTGGAACTAGCAGTGACGTTGGTGGTTATTACTATGTCTCCTGGTCCCAACACCACCCAGGCAAAGC16740.16485350786402442No Hit
GTCGATCACCATCTCCTGCACTGGAACTAGCAGTGACGTTGGTGGTTATTACTATGTCTCCTGGTCCCAACACCA16490.16239153791384484No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG15710.15471019166928457No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG15280.15047560335497567No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG14880.14653645143468838No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG14720.14496079066657344No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT14670.14446839667653752No Hit
GGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC14570.1434836086964657No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG13900.13688552922998443No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC13070.12871178899538824No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC12920.1272346070252805No Hit
CTTTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTC12290.12103044275082796No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA12220.12034109116477769No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA12110.11925782438669866No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT11590.11413692689032513No Hit
GGTTATTACTATGTCTCCTGGTCCCAACACCACCCAGGCAAAGCCCCCAAACTCATAATTTATGATGTCAGTAAT11440.1126597449202174No Hit
GACATAAAGAGTTCTGCTGCTTGTATATGAGCTGCAGTAATAATCAGCCT11290.11118256295010966No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC11170.11000081737402347No Hit
CAGTAATACACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGA11090.109212986989966No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC11090.109212986989966No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11080.10911450819195881No Hit
CTATTATAGTGGGAGCACCTACTACAACCCGTCCCTCAAGAGTCGAGTCACCATATCCGTAGACACGTCCATGAA11070.10901602939395164No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT10810.10645558064576487No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC10580.10419056829159966No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC10570.10409208949359247No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT10520.10359969550355656No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG10380.10222099233145601No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTAGTAGTTACTACTGGG10320.1016301195434129No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGAT10550.064.2122270
TACGTAT350.003650861.7725470
CTATACG200.00770625351.1495864
GGTATCA9550.045.0212551
CCGAAAC455.0754235E-543.01736568
CTACGGA11000.042.42057469
GTATCAA23200.036.3294941
TCTACGG12200.033.9555168
ACGTGTC12750.033.06859263
GACGTGT12600.032.8121462
AAAGCTA751.4584966E-632.76053255
AATGGTA5500.032.75816770
CTGGAGT12150.032.6233670
TGTCTAC12400.032.46402466
TGGACGT12200.032.27608560
GTGTCTA12650.032.13494565
TATCAAC26450.031.8483032
TGGTTCA12500.031.37218753
ATGGACG12650.031.05314859
AAAACCG1402.8580735E-530.8862770
Produced by FastQC (version 0.11.5)