FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692580

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692580
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1620657
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT38180.23558347016055833No Hit
GTATTACTGTCAGCAATACAGTACCTCTCAGTACACTTTTGGCCAGGGGA35280.21768949259466994No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG33470.2065211824587189No Hit
ATACAGTACCTCTCAGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT30320.18708462061990908No Hit
GTACTGTATTGCTGACAGTAATACATTGCAGAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC27180.16770976215201613No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25870.1596266205619079No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC25650.1582691464017371No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25120.15499886774314367No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25100.15487546100130994No Hit
CAGTAATACATTGCAGAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA24320.15006259806979513No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATTGCA22740.1403134654649318No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20980.12945367218356507No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20850.12865152836164592No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTCTGCAATGTATTACTGTCAGCAATA20030.12359185194646367No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT19170.11828536204761403No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT19100.11785343845119602No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18960.11698959125836003No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17140.10575957775149215No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT16380.10107012156181105No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19900.042.7763631
CCTAACG603.342211E-739.5483972
GTATCAA55050.036.8406331
CTAACGC701.1180382E-633.8965343
TATCAAC61150.033.0393832
ATCAACG61200.032.678033
TCAACGC61550.032.2659454
CAACGCA62450.031.8561975
CGCAGAG65300.030.5168028
ACGCAGA66150.030.073447
AGAGTAC66900.029.68563711
AACGCAG67200.029.553116
GCAGAGT67900.029.0987039
GTGGTAT6300.029.0694261
TGGTATC6500.028.1619362
CAGAGTA71850.027.5461510
GATCGTA500.00797914427.42536737
GCGTATT550.00888181226.829266
AATGGTA10550.026.70635870
TCCGTCA859.5919607E-426.0765569
Produced by FastQC (version 0.11.5)