FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692581

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692581
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1449909
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC44480.3067778736458633No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT41510.2862938294748153No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC32570.22463478742459012No Hit
AACCAGGGACAGCCCCTAAGCTCCTGATCTATGCTGCATCTACTTTGCAA26780.18470124676790062No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG25740.17752838281574912No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC23520.16221707707173347No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23270.16049283092938937No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22580.15573391157651964No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21770.15014735407532473No Hit
GTGTATTGTCACTCTTTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGGCGATGGTGAGAGTGAAAT21630.14918177623561205No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21630.14918177623561205No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT21040.14511255533967996No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG20500.14138818367221667No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19800.13656029447365317No Hit
GTCCCTGGTTTGTGCTGATACCAATTCAAAGAGACGCCAATGCTCTGACTTGCCCGGCAAGTGATGGTGACT19670.13566368647963425No Hit
GAGGTAGAGCGCACACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT19520.13462913879422778No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19300.13311180218896496No Hit
GTAGTATATGGCCGTGTCCTCGGCTCTCAAGGTGTTCATTTGCAAATACA18860.13007712897843934No Hit
CTCTACCTCGGTCCCTCCGCCGAAAGTGAGAGGTGTATTGTCACTCTTTT18860.13007712897843934No Hit
GATCTATGCTGCATCTACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCACTGGATCTGGGACAGATTTCA18690.12890464160164533No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTGGCGTCTCTTTGAATTGGTATCAGCACAAA18590.12821494314470772No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18440.12718039545930124No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18230.12573202869973218No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17950.1238008730203068No Hit
ATATACTACTGTGCGAAAAATGGGCCCACACAGTTATGGTCACTGGCCCA17320.11945577274159966No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17250.1189729838217433No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG17190.11855916474758071No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT17100.11793843613633684No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC17020.11738667737078673No Hit
GTGCAGCCACAGTTCGTGTGCGCTCTACCTCGGTCCCTCCGCCGAAAGTG16480.11366230570332345No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16060.11076557218418535No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15680.10814471804782232No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15110.10421343684327775No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG14870.10255816054662742No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14660.10110979378705835No Hit
ACTTACTACTGTCAAAAGAGTGACAATACACCTCTCACTTTCGGCGGAGGGACCGAGGTAGAGCGCACACGAA14640.10097185409567085No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT14590.10062700486720201No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT350.001388176239.19325639
CGTATGC2350.038.1314841
GTATCAA43350.036.618681
TCGTATG2200.035.9388640
TCTGGAG9350.035.65746370
CGAGACG2650.035.5477223
ATCTCGT2250.034.8929937
GTATGCC2650.033.89377242
GCCGTCT2300.033.35553746
ATGCCGT2450.032.57594344
ACGAGAC3050.030.8846622
TACGTAT1801.8810533E-730.87011770
TATCAAC50550.030.8031182
CCGTCTT2500.030.75748347
CGAGCTT1154.064156E-430.19902870
ATCAACG52500.029.9152363
CGGGTAT902.4952533E-729.89491317
TAATACG450.005237656629.893884
TCAACGC52800.029.7452624
CAACGCA54000.029.1465365
Produced by FastQC (version 0.11.5)