FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692583

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692583
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences827427
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAATCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGG39840.48149262714414687No Hit
GATTATTACTGTGCAGCATGGGATGACAGCCTGAGTGGTCCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTC36660.4430602337124605No Hit
CCCAAGGACCACTCAGGCTGTCATCCCATGCTGCACAGTAATAATCAGCC31300.37828110516093866No Hit
GTCCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC26760.3234122164251348No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC26560.32099508476276456No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA25810.31193084102887625No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT24290.2935606403948626No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG21170.25585338646188727No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC19170.23168206983818512No Hit
AGTCTGTACTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGG18500.22358467876924493No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA17590.21258672970546041No Hit
GTACTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC16590.20050107139360937No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG16580.20038021481049084No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGGACCACTC16400.19820479631435764No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG15340.1853939985037955No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC14790.1787468864322774No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT14100.17040778219710018No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC14090.17028692561398165No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG13880.16774893736849292No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13570.1640023832918191No Hit
GTGCAGGGTCGTGGGCCCAGTCTGTACTGACTCAGCCACCCTCAGCGTCT13550.16376067012558207No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG13210.1596515462995527No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC12740.1539712868929827No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT12470.1507081591487829No Hit
GTATACACTATTACTTCCGATGTTGGAGCTGCTTCCAGAACAAGAGATGGTGACCCTCTGCCCGGGGGTCCCAGA12260.1481701709032942No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC12160.14696160507210906No Hit
GTGTATACTGGTACCAGCAGCTTCCAGGAACGACCCCCAAACTCCTCATC12130.14659903532275353No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC12110.14635732215651653No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA11850.14321505099543524No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG11840.14309419441231672No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG11760.14212734174736866No Hit
CTATTACTTCCGATGTTGGAGCTGCTTCCAGAACAAGAGATGGTGACCCT11720.1416439154148946No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC11720.1416439154148946No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA11140.134634233594021No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC10150.12266943186528842No Hit
GTAATAAGCAGCCGTGTCCTCGGCTCTCAGGCTGTCCATTTGCAGAAACA10000.12085658311851075No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC9730.11759345537431097No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA9640.11650574612624437No Hit
GATTATCCCTATAGATGAGGAGTTTGGGGGTCGTTCCTGGAAGCTGCTGG9540.11529718029505925No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGGACCACTCA9480.11457204079634821No Hit
GCATGGGATGACAGCCTGAGTGGTCCTTGGGTGTTCGGCGGAGGGACCAA9420.11384690129763714No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT9330.11275919204957054No Hit
GTCGTGGGCCCAGTCTGTACTGACTCAGCCACCCTCAGCGTCTGGGACCC9260.11191319596774096No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG9210.1113089130521484No Hit
GCTTATTACTGTGCGAGAGTGAGAAGTAGCCATTCTCTAAATGATGGCTT9200.1111880564690299No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA9150.11058377355343733No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG8900.10756235897547456No Hit
CCCCTGTGGGCCGCTGATTATCCCTATAGATGAGGAGTTTGGGGGTCGTTCCTGGAAGCTGCTGGTACCAGTATA8790.10623293656117096No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC8630.10429923123127478No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT8520.10296980881697117No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT8400.10151952981954904No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC8360.101036103487075No Hit
ACCCTGCACAGTGAGTGAGGAGGGTGAGGAGGAGAGGGAAGCCGGCCATG8320.10055267715460095No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)