FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692584

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692584
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences827427
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGCAGCATGGGATGACAGCCTGAGTGGTCCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTC34170.41296694451595123No Hit
GTCCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC27530.3327181733252601No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26850.3244999256732014No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC25480.3079425737859654No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG22930.2771241450907452No Hit
AGTCTGTACTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGG22480.27168559885041216No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT20890.252469402134569No Hit
GTGCAGGGTCGTGGGCCCAGTCTGTACTGACTCAGCCACCCTCAGCGTCT20300.24533886373057684No Hit
GTACTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC19850.23990031749024388No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA19070.23047350400700004No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA16720.20207220697415No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG16530.19977593189489826No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG16400.19820479631435764No Hit
GTGTATACTGGTACCAGCAGCTTCCAGGAACGACCCCCAAACTCCTCATC16180.1955459514857504No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15920.19240368032466912No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC15640.18901969599735083No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC15510.18744856041681018No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT15090.18237258392583272No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA14720.17790089035044784No Hit
CCCAAGGACCACTCAGGCTGTCATCCCATGCTGCACAGTAATAATCAGCC13870.16762808078537442No Hit
GTAATAATCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGG13600.16436495304117463No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13450.16255210429439698No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG13290.1606183989645008No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC13280.16049754238138228No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG13170.15916811996707866No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC12840.15517985272416782No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG12680.15324614739427164No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG11910.14394019049414633No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA11680.14116048908242057No Hit
GTCGTGGGCCCAGTCTGTACTGACTCAGCCACCCTCAGCGTCTGGGACCC11580.13995192325123545No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGGACCACTC11290.13644708234079866No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT11270.13620536917456164No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC11130.1345133770109025No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA10550.12750369519002885No Hit
GTATACACTATTACTTCCGATGTTGGAGCTGCTTCCAGAACAAGAGATGGTGACCCTCTGCCCGGGGGTCCCAGA10390.12556998986013268No Hit
CCTCACTCACTGTGCAGGGTCGTGGGCCCAGTCTGTACTGACTCAGCCAC10320.1247239937783031No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCGTGGGC10040.1213400094509848No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC9850.1190437343717331No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC9610.11614317637688883No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA9460.11433032763011118No Hit
AGTGTATACTGGTACCAGCAGCTTCCAGGAACGACCCCCAAACTCCTCAT9430.11396775788075565No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT9350.11300090521580755No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC9250.11179233938462246No Hit
GAAGTAATAGTGTATACTGGTACCAGCAGCTTCCAGGAACGACCCCCAAA9150.11058377355343733No Hit
CTATTACTTCCGATGTTGGAGCTGCTTCCAGAACAAGAGATGGTGACCCT9090.10985863405472629No Hit
GATTATCCCTATAGATGAGGAGTTTGGGGGTCGTTCCTGGAAGCTGCTGG9010.10889178138977819No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA8910.10768321555859309No Hit
GCCCACAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAG8840.10683721947676351No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC8780.10611207997805244No Hit
CCCCTGTGGGCCGCTGATTATCCCTATAGATGAGGAGTTTGGGGGTCGTTCCTGGAAGCTGCTGGTACCAGTATA8760.10587036681181541No Hit
GTAATAAGCAGCCGTGTCCTCGGCTCTCAGGCTGTCCATTTGCAGAAACA8530.10309066540008967No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT8520.10296980881697117No Hit
GTAATAGTGTATACTGGTACCAGCAGCTTCCAGGAACGACCCCCAAACTCCTCATCTATAGGGATAATCAGCGGC8500.10272809565073414No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA8480.10248638248449712No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC8440.10200295615202307No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)