FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692585

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692585
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1428270
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTATGGTGCCCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT80890.5663494997444461No Hit
CCATAATATTGCTGACAGTAATAAACTGCCACGTCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC72670.508797356242167No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT71960.503826307350851No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC63660.44571404566363504No Hit
GTTTTATCCAGCTCCGACAATAAGAACTACTTAGCTTGGTTCCAGCAGAA55470.38837194648070744No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC52720.3691178838734973No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG49300.3451728314674396No Hit
GTTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC46850.32801921205374335No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC44800.31366618356473214No Hit
GTTTATTACTGTCAGCAATATTATGGTGCCCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAA43910.3074348687573078No Hit
CTCCCAAGCCGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT42960.30078346531118066No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT41910.29343191413388225No Hit
AACCAGGACAGTCTCCCAAGCCGCTCATTTACTGGGCATCTACCCGGGAA39240.27473796971160913No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC38780.2715172901482213No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG38690.27088715719016715No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA37440.26213531055052613No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT37010.25912467530648964No Hit
GCTTGGGAGACTGTCCTGGTTTCTGCTGGAACCAAGCTAAGTAGTTCTTATTGTCGGAGCTGGATAAAACACT36230.2536635230033537No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG36080.25261330140659677No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG34200.2394505240605768No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG33520.2346895194886121No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT33210.23251906152198112No Hit
GTCCTGGTTTCTGCTGGAACCAAGCTAAGTAGTTCTTATTGTCGGAGCTG32600.22824816036183634No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT32560.22796810126936787No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATC32340.22642777626079102No Hit
GTCCCTGACCGGTTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC31930.2235571705629888No Hit
GTAATAAACTGCCACGTCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA31500.2205465353189523No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC30850.21599557506633899No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATCCAGCTCCGA30590.21417519096529367No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC29540.20682363978799528No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC29260.2048632261407157No Hit
GGATAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA28460.19926204429134547No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGACGTGGCAGTTTATTACTGTCAGCAATATTATGGTGCC28380.19870192610640844No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT28340.19842186701393996No Hit
CCACTGAACCGGTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAAT27700.19394092153444376No Hit
CCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCA27110.1898100499205332No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG26970.18882984309689343No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT26810.1877096067270194No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25380.17769749417127015No Hit
GGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC25100.17573708052399056No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTG23890.1672652929768181No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG23790.16656514524564683No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA23790.16656514524564683No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCC23780.1664951304725297No Hit
GTAAATGAGCGGCTTGGGAGACTGTCCTGGTTTCTGCTGGAACCAAGCTA23500.16453471682525012No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG23170.1622242293123849No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA22550.157883313379123No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATCCAGCTCCGACAATAAGAACTA22020.1541725304039152No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT21650.15158198379858148No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT21370.14962157015130192No Hit
CATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGGTTCAGTGGCAGCGGGTCTGGGACAGATTTCA21310.14920148151259915No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC20920.14647090536103116No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA20040.14030960532672393No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT19980.1398895166880212No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC19820.13876928031814711No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA19490.1364587928052819No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT19430.13603870416657915No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC19170.13421832006553383No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA18960.13274800983007415No Hit
GAACTACTTAGCTTGGTTCCAGCAGAAACCAGGACAGTCTCCCAAGCCGC18770.1314177291408487No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG18640.13050753709032606No Hit
CACCATCAGCAGCCTGCAGGCTGAAGACGTGGCAGTTTATTACTGTCAGCAATATTATGGTGCCCCGATCACCTT18510.12959734503980339No Hit
ACTTAGCTTGGTTCCAGCAGAAACCAGGACAGTCTCCCAAGCCGCTCATT18350.12847710866992934No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG18300.1281270348043437No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG18220.1275669166194067No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATGAGCGGCTTGGGAGACTGTCCTGGTTTCTGCTGGAA18210.12749690184628956No Hit
CGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT18120.12686676888823542No Hit
GTCGTGTCCCTTGGCCGAAGGTGATCGGGGCACCATAATATTGCTGACAGTAATAAACTGCCACGTCTTCAGC18060.12644668024953265No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA18060.12644668024953265No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT18050.12637666547641552No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA18010.126096606383947No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG17980.12588656206459564No Hit
GGCTGAAGACGTGGCAGTTTATTACTGTCAGCAATATTATGGTGCCCCGATCACCTTCGGCCAAGGGACACGA17780.12448626660225308No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA17710.12399616319043318No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT17650.12357607455173042No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT17610.1232960154592619No Hit
ATGGTGCCCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGA17300.12112555749263096No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC17260.12084549840016244No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG17220.12056543930769392No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC17220.12056543930769392No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC17200.12042540976145966No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC17180.12028538021522542No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC16750.11727474497118892No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGACG16420.11496425745832371No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA16300.11412408018091817No Hit
CAGTAATAAACTGCCACGTCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA16270.11391403586156679No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC16020.11216366653363859No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT15990.11195362221428722No Hit
GTCTCCCAAGCCGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGGTTCAGTGGCAGCGGGT15960.11174357789493583No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG15930.11153353357558445No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC15850.11097341539064742No Hit
CAGTAATACACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATA15440.10810280969284519No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC15290.10705258809608827No Hit
GCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATC15080.10558227786062858No Hit
CTACTTAGCTTGGTTCCAGCAGAAACCAGGACAGTCTCCCAAGCCGCTCATTTACTGGGCATCTACCCGGGAATC15050.10537223354127723No Hit
GTAATACACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA14650.1025716426165921No Hit
CTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGGTTCAG14650.1025716426165921No Hit
GGCCGAAGGTGATCGGGGCACCATAATATTGCTGACAGTAATAAACTGCC14520.10166145056606944No Hit
GGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT14340.10040118464996114No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATCGC150.002409037768.61120633
TACGACA200.00762224651.2926813
GGTATCA11050.050.1409841
CGTATGC3000.046.00904541
TCGTATG2950.045.582140
CGAGACG3250.044.2045423
GTGGTAT2300.043.123131
CTTATAC3400.042.248421
TGGTATC2450.041.875972
TACGTAT650.001031517341.6301470
ATGCCGT3350.041.33354644
ACGAGAC3500.041.04132522
ATCTCCG3500.041.03413810
TATGCCG3400.040.67668543
ATCTCGT3050.040.59594337
GTATCAA30200.040.5430341
AGACGGA3600.039.9166625
TCTCCGA3650.039.34780511
TTTAGGC350.001363779939.33488538
TGGCGTA350.001407313739.084245
Produced by FastQC (version 0.11.5)