FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692586

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692586
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1428270
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATAATATTGCTGACAGTAATAAACTGCCACGTCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC81830.5729308884174561No Hit
ATATTATGGTGCCCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT78630.5505261610199752No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT77960.5458351712211276No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC53470.3743689918572819No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC53210.37254860775623655No Hit
GTTTTATCCAGCTCCGACAATAAGAACTACTTAGCTTGGTTCCAGCAGAA47180.33032969956660857No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC46920.3285093154655632No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT45890.3212977938344991No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG44850.3140162574303178No Hit
GTTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC43440.30414417442080277No Hit
GTAATAAACTGCCACGTCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA42350.2965125641510359No Hit
GTTTATTACTGTCAGCAATATTATGGTGCCCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAA41470.2903512641167286No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC40370.2826496390738446No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT35980.2519131536754255No Hit
GCTTGGGAGACTGTCCTGGTTTCTGCTGGAACCAAGCTAAGTAGTTCTTATTGTCGGAGCTGGATAAAACACT35380.24771226728839785No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA35350.2475022229690465No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT34890.24428154340565858No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC33090.2316788842445756No Hit
CTCCCAAGCCGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT32960.23076869219405294No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG32670.22873826377365625No Hit
AACCAGGACAGTCTCCCAAGCCGCTCATTTACTGGGCATCTACCCGGGAA32400.2268478648994938No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT32290.22607770239520536No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT31970.22383722965545733No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG30850.21599557506633899No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG30590.21417519096529367No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC30570.21403516141905943No Hit
GTCCTGGTTTCTGCTGGAACCAAGCTAAGTAGTTCTTATTGTCGGAGCTG30240.2117246739061942No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG29690.2078738613847522No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA29080.20360296022460736No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28950.20269276817408474No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGACGTGGCAGTTTATTACTGTCAGCAATATTATGGTGCC27900.19534121699678633No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATC27790.1945710544924979No Hit
GTCCCTGACCGGTTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC27330.19135037492911006No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT26550.18588922262597407No Hit
GTCCCAGACCCGCTGCCACTGAACCGGTCAGGGACCCCGGATTCCCGGGT26530.18574919307973983No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATCCAGCTCCGA26520.1856791783066227No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCC26300.18413885329804588No Hit
GGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC26170.1832286612475232No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCACTGAACCGGTCAGGGACCCCG25400.1778375237175044No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTG24610.1723063566412513No Hit
CCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCA23990.16796544070798938No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG23750.16628508615317833No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT23620.16537489410265566No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA22960.1607539190769252No Hit
CCACTGAACCGGTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAAT22900.16033383043822247No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG22700.15893353497587992No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22440.1571131508748346No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT22100.15473264858885225No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC22060.15445258949638374No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG21920.15347238267274396No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA21900.1533323531265097No Hit
GTCGTGTCCCTTGGCCGAAGGTGATCGGGGCACCATAATATTGCTGACAGTAATAAACTGCCACGTCTTCAGC21520.15067179174805884No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA21280.14899143719324778No Hit
GTAAATGAGCGGCTTGGGAGACTGTCCTGGTTTCTGCTGGAACCAAGCTA20810.14570074285674278No Hit
CAGTAATAAACTGCCACGTCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA20440.14311019625140906No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA20070.14051964964607533No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG19970.13981950191490405No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC19970.13981950191490405No Hit
GGCTGAAGACGTGGCAGTTTATTACTGTCAGCAATATTATGGTGCCCCGATCACCTTCGGCCAAGGGACACGA19900.13932939850308415No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT19760.13834919167944437No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC19320.13526854166229074No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT19060.13344815756124542No Hit
GGATAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA19050.1333781427881283No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG18570.13001743367850618No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA18430.12903722685486638No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA18210.12749690184628956No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA18100.12672673934200118No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC18030.12623663593018128No Hit
GGCCGAAGGTGATCGGGGCACCATAATATTGCTGACAGTAATAAACTGCC18030.12623663593018128No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC17900.1253264438796586No Hit
CACCATCAGCAGCCTGCAGGCTGAAGACGTGGCAGTTTATTACTGTCAGCAATATTATGGTGCCCCGATCACCTT17880.12518641433342434No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATCCAGCTCCGACAATAAGAACTA17710.12399616319043318No Hit
CATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGGTTCAGTGGCAGCGGGTCTGGGACAGATTTCA17510.12259586772809064No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT17290.12105554271951381No Hit
CGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT17230.12063545408081106No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC17170.1202153654421083No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATGAGCGGCTTGGGAGACTGTCCTGGTTTCTGCTGGAA17040.11930517339158564No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT16960.11874505520664862No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC16960.11874505520664862No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC16890.11825495179482871No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC16400.11482422791208945No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT16290.11405406540780105No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGACG15940.11160354834870159No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAACCGGTCAGGGACC15930.11153353357558445No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC15870.11111344493688169No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC15730.11013323811324191No Hit
GAACTACTTAGCTTGGTTCCAGCAGAAACCAGGACAGTCTCCCAAGCCGC15630.10943309038207061No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC15430.10803279491972806No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT15370.10761270628102529No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT15360.10754269150790817No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG15310.10719261764232253No Hit
ATGGTGCCCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGA15100.10572230740686285No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA15000.10502215967569156No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACATCGTGATGA14980.10488213012945732No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA14930.10453205626387169No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT14690.1028517017090606No Hit
ACTTAGCTTGGTTCCAGCAGAAACCAGGACAGTCTCCCAAGCCGCTCATT14690.1028517017090606No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG14690.1028517017090606No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT14500.10152142101983519No Hit
GCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATC14420.10096130283489817No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG14380.10068124374242966No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACCG252.7163912E-454.573166
CGTCTTA250.006344834353.72715469
GTGGTAT2850.051.486771
TCGTACC200.00769930251.162339
GGTATCA11750.047.048871
TCGCTAT500.00818719842.6799970
TGGTATC3450.042.5161632
CCGTTAA350.001423306438.9944816
GTATCAA31350.038.859991
TACTTCG400.002304804335.34852260
TCTACGA400.00260343834.48132740
GCTTGTA601.4471318E-534.1249541
CAAGGTA601.4526953E-534.1058354
CTCGCTA400.002747036534.10583512
TATCAAC36350.033.1264572
ATCAACG37300.032.0964783
TCAACGC37550.031.8805524
CAACGCA38500.031.1835695
AATGGTA4600.030.92752870
AACGCAG39150.030.754036
Produced by FastQC (version 0.11.5)