FastQCFastQC Report
Wed 8 May 2024
EGAF00004692589

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692589
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1638548
Total Bases120.7 Mbp
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT49820.3040496830120326No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC48220.29428494008109624No Hit
GTATATTACTGTGCGAGAGCCGGCGGTGGATACACCTATGGTTACTACCA40640.2480244704457849No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTC38090.232461911399605No Hit
GTATGATGACTGGCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCA35070.21403095911746253No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC33830.20646328334598682No Hit
CATCTATGGTGCATCAACTAGGGCCACTGGTGTCCCAGCCAGGTTCAGTG33440.20408312725657107No Hit
GTCTGGGACAGAGTTCACTCTTACCATCGGTAGCCTGCAGCCTGAAGATT33210.20267944546024896No Hit
GATCAGCACTGAGCACTGAGGACTCACCATGGAGTTTTGGCTGAGCTGGG32190.196454421841777No Hit
GTCATCATACTGCTGGCAGTGATAAACTGCCAAATCTTCAGGCTGCAGGC31750.19376911753576948No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG31260.1907786650131702No Hit
GCCCAGCACTGGGCTTCCGAGGTGTTTCCATTCGGTGATCAGCACTGAGC31250.19071763536985184No Hit
GAGTTCACTCTTACCATCGGTAGCCTGCAGCCTGAAGATTTGGCAGTTTA31100.18980219072007656No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC30850.18827644963711773No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC30010.18314995959837613No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG29990.1830279003117394No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA29180.17808449920295286No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC29170.1780234695596345No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGA28590.17448375024717006No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT28400.17332418702412133No Hit
CCCTATATCTTCAGATGACCAAGCTGACAAAGGAAGACACGGCCGTATAT27270.1664278373291475No Hit
CCATAGATGAGGAGCCTGGGAGCCTGACCAGGTTTTCGCTGATACCAGGC27000.164780036959552No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG26440.16136237693372427No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA26230.16008075442403885No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG26170.1597145765641287No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGACACCAGTGGCCCTAGT25960.15843295405444333No Hit
GTCTCGAGACACCCTGTCTGTCTCTCCTGGGGAAAGAGTCACCCTCTCCT25150.15348955294565678No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC24980.15245204900924478No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT24280.1481799739769601No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT23960.1462270253907728No Hit
AGCACATACTACGCAGACTCCGTGAGGGGCCGCTTCACCATCTCCAGAGA23810.1453115807409975No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT23570.14384686930135707No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT23560.1437858396580387No Hit
CTCCTGTTGGGCCACTCAGAGTGTTAGGAGCAACTTGGCCTGGTATCAGC23310.1422600985750799No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG23120.14110053535203118No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA23000.14036817963221096No Hit
CTCCTGTGCAGCCTCTGGACTCAGCGTCAGCAGCAATTACATGAGCTGGG22270.13591301566997122No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22160.13524168959346933No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22010.13432624494369405No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG21900.13365491886719216No Hit
GCACTGAGCACTGAGGACTCACCATGGAGTTTTGGCTGAGCTGGGTTTTC21850.1333497706506004No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG21840.13328874100728205No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC21780.13292256314737194No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT21150.12907769561831572No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20960.12791813239526703No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT20400.12450047236943929No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTC20390.12443944272612092No Hit
CACCTATGGTTACTACCACTACTACATGGACGTCTGGGGCAAAGGGACTA20240.12352399807634565No Hit
GTATGTGCTACTGCCACTAGAAATCACTGAGACCCATTCCAGCCCCTTTC20220.12340193878970893No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC19640.11986221947724449No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCA19560.11937398233069767No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTG19310.11784824124773884No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGACACCA19170.11699382624128192No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19080.11644455945141674No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT19040.11620044087814332No Hit
GTGTTAGGAGCAACTTGGCCTGGTATCAGCGAAAACCTGGTCAGGCTCCC18970.11577323337491487No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC18780.11461367015186616No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCCTTTGATCTCCACCTTGGTCC18330.1118673362025403No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC18320.11180630655922194No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG18080.11034159511958147No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAG18060.11021953583294478No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATC17770.10844967617671256No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG17520.10692393509375372No Hit
GATATAGGGTGTTCTTGGAATTGTCTCTGGAGATGGTGAAGCGGCCCCTC17150.10466583829097469No Hit
ATGCAGGAGAAGGTGTCCCCCTTCTTCCAGTCCTCGGCTGCCACGCGCAG16970.10356730471124435No Hit
GTGTGAGGAGCACCTGGTGGAGACTGGAGGAGGCTTGATCCAGCCTGGGG16970.10356730471124435No Hit
GAGTGTTAGGAGCAACTTGGCCTGGTATCAGCGAAAACCTGGTCAGGCTC16900.10314009720801588No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG16860.10289597863474248No Hit
GTGCAGCCACAGTTCCTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG16850.10283494899142412No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC16820.10265186006146905No Hit
ATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGCCCACCATGCA16770.10234671184487729No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT16580.10118714862182861No Hit
GTAATATACGGCCGTGTCTTCCTTTGTCAGCTTGGTCATCTGAAGATATA16570.10112611897851025No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)