FastQCFastQC Report
Wed 8 May 2024
EGAF00004692590

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692590
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1638548
Total Bases120.6 Mbp
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT49390.30142540834934345No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC44030.26871351953070644No Hit
GTATATTACTGTGCGAGAGCCGGCGGTGGATACACCTATGGTTACTACCA39200.23923620180794214No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTC35450.21635008556355995No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG35010.2136647812575524No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC34370.20975888408517784No Hit
GTCTGGGACAGAGTTCACTCTTACCATCGGTAGCCTGCAGCCTGAAGATT33220.2027404751035673No Hit
GTATGATGACTGGCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCA32930.20097061544733508No Hit
CATCTATGGTGCATCAACTAGGGCCACTGGTGTCCCAGCCAGGTTCAGTG31540.1924874950260841No Hit
GAGTTCACTCTTACCATCGGTAGCCTGCAGCCTGAAGATTTGGCAGTTTA30690.1872999753440241No Hit
GATCAGCACTGAGCACTGAGGACTCACCATGGAGTTTTGGCTGAGCTGGG30380.18540805640115518No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC30360.18528599711451846No Hit
GTCATCATACTGCTGGCAGTGATAAACTGCCAAATCTTCAGGCTGCAGGC30300.18491981925460835No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT29990.1830279003117394No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29950.18278378173846602No Hit
GCCCAGCACTGGGCTTCCGAGGTGTTTCCATTCGGTGATCAGCACTGAGC28160.1718594755844809No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG27260.16636680768582915No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27060.16514621481946212No Hit
GTAATATACGGCCGTGTCTTCCTTTGTCAGCTTGGTCATCTGAAGATATA25560.15599176832170922No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC25200.15379470116224853No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA25180.15367264187561183No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT25150.15348955294565678No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG24910.1520248415060163No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24280.1481799739769601No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC24280.1481799739769601No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGA24260.1480579146903234No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT24160.14744761825713984No Hit
CCCTATATCTTCAGATGACCAAGCTGACAAAGGAAGACACGGCCGTATAT24050.14677629218063798No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCA24010.14653217360736456No Hit
CTCCTGTTGGGCCACTCAGAGTGTTAGGAGCAACTTGGCCTGGTATCAGC23580.1439078989446754No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGACACCAGTGGCCCTAGT23570.14384686930135707No Hit
CCATAGATGAGGAGCCTGGGAGCCTGACCAGGTTTTCGCTGATACCAGGC23440.14305348393821846No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA23180.1414667132119413No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT22700.13853729033266038No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22400.1367064010331098No Hit
GTCTCGAGACACCCTGTCTGTCTCTCCTGGGGAAAGAGTCACCCTCTCCT22330.13627919352988133No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC21910.13371594851051052No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG21430.1307865256312296No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT21040.12840636954181384No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20880.1274298952487202No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20750.12663650988558164No Hit
GTATGTGCTACTGCCACTAGAAATCACTGAGACCCATTCCAGCCCCTTTC20410.12456150201275763No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG20320.12401223522289245No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT20250.123585027719664No Hit
AGCACATACTACGCAGACTCCGTGAGGGGCCGCTTCACCATCTCCAGAGA20220.12340193878970893No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC20200.12327987950307223No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGACACCA19960.12181516806343178No Hit
GCACTGAGCACTGAGGACTCACCATGGAGTTTTGGCTGAGCTGGGTTTTC19580.11949604161733438No Hit
GTGTTAGGAGCAACTTGGCCTGGTATCAGCGAAAACCTGGTCAGGCTCCC19330.11797030053437557No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC19280.1176651523177838No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG19020.11607838159150663No Hit
CACCTATGGTTACTACCACTACTACATGGACGTCTGGGGCAAAGGGACTA18970.11577323337491487No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG18540.11314895871222572No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAG18150.11076880262280994No Hit
CTCCTGTGCAGCCTCTGGACTCAGCGTCAGCAGCAATTACATGAGCTGGG18030.1100364469029897No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG18020.10997541725967137No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC17980.10973129868639797No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTC17910.1093040911831695No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGC17700.1080224686734841No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT17640.10765629081357396No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT17330.10576437187070503No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCCTTTGATCTCCACCTTGGTCC17200.10497098650756645No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17010.10381142328451776No Hit
GTGCAGCCACAGTTCCTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG16870.10295700827806081No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATC16740.10216362291492224No Hit
GATATAGGGTGTTCTTGGAATTGTCTCTGGAGATGGTGAAGCGGCCCCTC16510.10075994111860014No Hit
GCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGG16440.10033273361537166No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA16400.10008861504209823No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)